The role of phytochromes in Nicotiana tabacum against Chilli veinal mottle virus

Fei, Chunyan; Chen, Lijuan; Yang, Ting; Zou, Wenshan; Lin, Honghui; Xi, Dehui

doi:10.1016/j.plaphy.2019.04.002

Cited by 18 publications

(17 citation statements)

References 34 publications

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“…Both light intensity and light quality can affect the expression of the PR gene in plant-pathogen interaction systems [24]. Phytochromes positively regulated Nicotiana tabacum against CMV [26] and ChiVMV [27] via a salicylic acid-dependent pathway. In addition, changes in the light conditions can also affect the JA pathway, which modulates the plant resistance to pathogens, such as Podosphaera xanthii [45], Pseudomonas syringae [46], Magnaporthe grisea [25], and Botrytis cinerea [23].…”

Section: Resultsmentioning

confidence: 99%

“…The accumulation of pathogenesis-related 1 (PR1) protein in the rice phytochrome triple mutant ( phyAphyBphyC ) was significantly attenuated, and the plants were more sensitive to blast fungus [25]. Compared to wild-type plants, phytochrome-deficient Nicotiana tabacum has reduced resistance to cucumber mosaic virus (CMV) [26] and chilli veinal mottle virus (ChiVMV) [27].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade

Zhang

Shang

Wang

et al. 2019

Viruses

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Shading in the intercropping system is a major abiotic factor which influences soybean growth and development, while soybean mosaic virus (SMV) is a biotic factor that limits the yield and quality of soybean. However, little is known about the defense response of soybean to SMV in the shade. Thus, in the current study, both intensity and quality (red:far-red, R:FR) of the light were changed to simulate the shaded environment and comparative transcriptome analysis was performed. Morphologically, plant growth was inhibited by SMV, which decreased 35.93% of plant height and 8.97% of stem diameter in the shade. A total of 3548 and 4319 differentially expressed genes (DEGs) were identified in soybean plants infected with SMV under normal light and in the shade. Enrichment analysis showed that the plant defense-related genes were upregulated under normal light but downregulated in the shade. Pathways that were repressed include plant-pathogen interaction, secondary metabolism, sugar metabolism, and vitamin metabolism. In addition, genes associated with signaling pathways such as salicylic acid (SA), jasmonic acid (JA), and ethylene (ETH) were also downregulated in the shade. A qRT-PCR assay of 15 DEGs was performed to confirm transcriptome results. According to our knowledge, this is the first report on soybean response to dual stress factors. These results provide insights into the molecular mechanisms in which soybean plants were infected with SMV in the shade.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade

Zhang

Shang

Wang

et al. 2019

Viruses

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“…Therefore, it is of great significance to timely identify and detect plant viruses. ChiVMV found in pepper, tomato and tobacco plants spreads rapidly in recent years, and its scope of damage has increasingly expanded [17,34]. Sichuan province is one of the main tobaccoproducing regions in China, with a large number of pepper plants scattered in the tobacco area.…”

Section: Discussionmentioning

confidence: 99%

Characterization and a RT-RPA assay for rapid detection of Chilli Veinal mottle virus (ChiVMV) in tobacco

Jiao

et al. 2020

Virol J

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Background: Chilli veinal mottle virus (ChiVMV), which belongs to the genus Potyvirus of the family Potyviridae, mainly infects solanaceous plants and has caused serious economic losses in Asia and Africa. Tobacco plants infected with ChiVMV suffered from punctate necrosis of leaves, leaf deformation, systemic necrosis of leaves and stems, and eventually plant death. However, ChiVMV infection could not usually be identified given the lack of rapid and efficient detection assays in tobacco plants. Therefore, an isolate of tobacco-infecting ChiVMV (ChiVMV-LZ) was obtained, and a novel isothermal amplification and detection technique, reverse transcription-recombinase polymerase amplification (RT-RPA), was established to detect ChiVMV in tobacco plants. Methods: In this study, the full-length genome of ChiVMV-LZ was obtained using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) assays. The genome sequence of ChiVMV-LZ was characterized by sequence alignment and phylogenetic analysis. Then, a RT-RPA assay was established for rapid and sensitive detection of ChiVMV-LZ in tobacco. Additionally, the established RT-RPA assay was compared to the RT-PCR assay in aspect of sensitivity and application in field-collected tobacco samples. Results: ChiVMV-LZ was isolated from diseased tobacco in Luzhou, Sichuan, China. The tobacco plants inoculated with ChiVMV-LZ showed typical symptoms of yellow and round spots on the leaves, and curled and folded leaf margin, similar to those observed on naturally ChiVMV-infected tobacco in the field. The full-length genomic sequence of ChiVMV-LZ was determined to be 9742 nucleotides. Sequence alignment and phylogenetic analysis showed that ChiVMV-LZ was most closely related to ChiVMV-Yp8 isolated from pepper plants in Sichuan province while distantly related to ChiVMV-YN from tobacco in Yunnan province, indicating a possibly geographical differentiation of ChiVMV isolates. Additionally, a RT-RPA assay was established for rapid detection of ChiVMV in tobacco. The RT-RPA has no cross-reaction with other related tobacco viruses and is about 10-fold more sensitive than conventional RT-PCR method. Conclusion: The characterization of ChiVMV-LZ infecting tobacco was determined, and the established RT-RPA assay provides a reliable and effective method for rapid detection of ChiVMV in tobacco.

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“…First time in history, the ChiVMV reported from Malaysia (Ong et al 1979) and then it spread to Asia and Africa (Lee et al 2013). The yield losses of more than 50 percent have been recorded in Pakistan due to this disease virus (Hameed et al 1995).It is a ssRNA virus belongs to the family Potyviridae and the genus Potyvirus (Lee et al 2017;Fei et al 2019;Jiao et al 2020;Adediji et al 2020). This virus is transmitted from one plant to another through a vector aphid(Aphis gossypii) in a non-persistent manner, through grafting, mechanically and did not transfer through seed (Shah et al 2008).The infected chilli plant could not survive happily and have shown the symptoms of vein mottling, stunting in an early stage of infection, dark-green streaks on branches and on the stem, flowers dropping and fruit deforming is the characteristic symptoms of ChiVMV (Wang et al 2006;Nono-Womdim et al 2001;Ahmad and Ashfaq 2018;Gao et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Role of Plant Extracts and Salicylic Acid for the Management of Chili Veinal Mottle Virus Disease

Ali¹,

Zeshan²,

Iftikhar³

et al. 2020

Pak. J. Phytopathol.

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The role of phytochromes in Nicotiana tabacum against Chilli veinal mottle virus

Cited by 18 publications

References 34 publications

Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade

Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade

Characterization and a RT-RPA assay for rapid detection of Chilli Veinal mottle virus (ChiVMV) in tobacco

Role of Plant Extracts and Salicylic Acid for the Management of Chili Veinal Mottle Virus Disease

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