The role of physicochemical interactions and FLO genes expression in the immobilization of industrially important yeasts by adhesion

Kuřec, Michal; Brányik, Tomáš

doi:10.1016/j.colsurfb.2011.02.004

Cited by 13 publications

(9 citation statements)

References 29 publications

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“…The total surface tension ( TOT ) of the tested solid surfaces and their components ( LW ,  + and  − ) were calculated from CA by using Young's equation [27]. The values of total free energy of adhesion between solid surfaces in water (ΔG TOT ) were calculated using the thermodynamic approach [29].…”

Section: Surface Tension/total Free Energymentioning

confidence: 99%

“…The microbial lawns obtained were then deposited on agar plates to stabilize moisture content, fixed to a glass slide and allowed to dry in air for 60 min. The CA was measured using the CAM 200 goniometer (KSV Instruments, Finland) according to [27]. Readings were performed for each liquid at equilibrium stage, i.e.…”

Section: Contact Angle (Ca)mentioning

confidence: 99%

“…The dehydrated cell powder was gently homogenized with a spatula and analyzed using X-ray photoelectron spectroscopy (ESCAProbeP, Omicron Nanotechnology GmbH, Germany). The cell wall composition was calculated according to [27] and expressed as atomic surface concentration ratios related to total carbon.…”

Section: X-ray Photoelectron Spectroscopy Analysis (Xps)mentioning

confidence: 99%

See 2 more Smart Citations

Cell Surface Determinants Important for Biofilm-Based Solid Substrate Degradation

Dostálková¹,

Jirků²,

Procházková³

et al. 2013

JBNB

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The study links targeted cell surface characterization to the quantified capacity of cellulose degrading Pseudomonas fluorescens cells to colonize a (similarly characterized) cellulosic carrier. The experiments were conducted to clarify the effect of cultivation conditions on the achieved state of this carrier colonization. The suggested approach seems to be sufficient to verify the right choice of cultivation medium as a major factor determining the binding complementarity between microbial cells and solid cellulose.

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Section: Surface Tension/total Free Energymentioning

confidence: 99%

Section: Contact Angle (Ca)mentioning

confidence: 99%

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Cell Surface Determinants Important for Biofilm-Based Solid Substrate Degradation

Dostálková¹,

Jirků²,

Procházková³

et al. 2013

JBNB

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“…Interestingly, higher salt conditions foster larger biomassadsorbent interactions as compared to low salt conditions, presumably due to the relatively hydrophobic nature of the adsorbent as well as an enhancement of hydrophobic and acid-base interactions in these conditions. Once again, biomass adhesion to the adsorbent is expected to increase with each successive growth stage as the FLO genes which encode cell-surface glycoproteins (adhesins) are activated, thereby facilitating the biofilm formation [25], however, relative differences in secondary minima of interaction energies (also at an interfacial distance of ca. 5 ~ 7 nm) for biomass phases are more pronounced at low salt concentrations (Fig.…”

Section: Biomass Depositionmentioning

confidence: 99%

“…H. polymorpha like other methylotrophic yeast strains has a rigid bilayer cell wall, mainly composed of mannoproteins linked convalently to glucans, to lesser extent chitin, and is located outside of the plasma membrane [21][22][23][24]. This cell wall/ surface undergo rearrangements during cell growth and this physicochemical/ or specific molecular character of the cell surface composition can be key to better understand the interaction to solid supports [25,26]. We have previously demonstrated the application of colloid theory methods, namely, extended Derjaguin, Landau, Verwey and Overbeek (xDLVO) theory, in successfully explaining these interactions [27].…”

Section: Introductionmentioning

confidence: 98%

Growth-dependent surface characteristics of Hansenula Polymorpha: implications for expanded bed adsorption chromatography

Naz

Dsouza

Yelemane

et al. 2015

Biotechnol Bioproc E

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The cell surface characteristics of a methylotrophic wild-type strain of yeast, Hansenula polymorpha, was investigated at different growth stages (early log, late log, stationary and death) of the biomass under different conditions (low and high salt in intact and disrupted forms) using extended DLVO theory. Biomass was characterized by contact angle measurements as well as zeta potential determinations. These measurements were used to describe the hydrophobic, polar, and electrostatic behavior of the biomass in its growth stages. Consequently, interaction free energy vs. distance profiles of the biomass with anionexchange and HIC adsorbents were conveniently generated. A strong interaction was calculated between cells and the adsorbents in the stationary and death phases of the biomass illustrated by the striking correlation between theoretical predictions and biomass deposition experiments. The physico-chemical properties of biomass in different growth phases have important implications for expanded bed adsorption chromatography, where unfavorable biomassadsorbent interactions adversely affect process efficiency.

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High gravity primary continuous beer fermentation using flocculent yeast biomass

et al. 2014

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The current work assessed a new immobilized cell reactor system throughout a long-term (54 days) continuous primary fermentation of lager-type wort of high specific gravity. The experiment was performed in a 4 L airlift bioreactor and immobilization of biomass was attained solely by flocculation. Despite the constant liquid agitation and washout of biomass, up to 53 g dry wt/L of yeast remained immobilized in the system. Two types of beer were produced without interrupting the reactor, based on two types of wort: a Pilsener type with high specific gravity of 15.6 ± 0.3°P; and a dark lager wort with specific gravity of 14.4 ± 0.03°P. Even during the inlet of high gravity wort, the desired attenuation was achieved without the need for either recirculation or an auxiliary second stage bioreactor. The specific saccharide consumption rate was kept around 7.9 ± 0.4 g/L/h and ethanol productivity oscillated at 3.36 ± 0.2 g/L/h for nearly a month. During this period the volumetric productivity of the current bioreactor reached 1.6 L beer/L/day. The green beers produced from the Pilsener and dark lager worts met the standards of regular finished primary beer fermentation. The productivity of diacetyl through the entire experiment could be correlated to the free amino nitrogen consumption rate.

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The role of physicochemical interactions and FLO genes expression in the immobilization of industrially important yeasts by adhesion

Cited by 13 publications

References 29 publications

Cell Surface Determinants Important for Biofilm-Based Solid Substrate Degradation

Cell Surface Determinants Important for Biofilm-Based Solid Substrate Degradation

Growth-dependent surface characteristics of Hansenula Polymorpha: implications for expanded bed adsorption chromatography

High gravity primary continuous beer fermentation using flocculent yeast biomass

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