• NOTICE: this is the author's version of a work that was accepted for publication in the journal, Appetite. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. Abstract: The effects of prolonged treadmill running on appetite, energy intake and acylated ghrelin (an appetite stimulating hormone) were examined in 9 healthy males over the course of 24 h. Participants completed 2 experimental trials (exercise and control) in a randomised -crossover fashion. In the exercise trial participants ran for 90 min at 68.8 ± 0.8% of maximum oxygen uptake followed by 8.5 h of rest. Participants returned to the laboratory on the following morning to provide a fasting blood sample and ratings of appetite (24 h measurement). No exercise was performed on the control trial. Appetite was measured within the laboratory using visual analogue scales and energy intake was assessed from ad libitum buffet meals. Acylated ghrelin was determined from plasma using an ELISA assay. Exercise transiently suppressed appetite and acylated ghrelin but each remained no different from control values in the hours afterwards. Furthermore, despite participants expending 5324 kJ during exercise there was no compensatory increase in energy intake (24 h energy intake; control 17191 kJ, exercise 17606 kJ). These findings suggest that large energy deficits induced by exercise do not lead to acute compensatory responses in appetite, energy intake or acylated ghrelin.Professor H.R Bertoud Louisiana State University Baton Rouge LA USA Dear Professor Berthold, Thank you for inviting us to re-submit our paper to be considered for publication in Appetite. We have carefully considered the comments from the reviewers and have formulated responses to the points raised. Our responses, and the necessary changes (highlighted in yellow) are included within the revised document. We thank you once more for considering our manuscript and we look forward to hearing from you in due course. We thank each reviewers for reading our paper and for their constructive comments. We feel that we can adequately address the issues raised by each reviewer therefore, in an effort to do so, below we have listed the comments from each reviewer followed by our responses.
Reviewer # 1 comment 1Studies on exercise and compensation in the short term have already taken place and have shown no compensation in energy intake once REI is calculated; therefore what is the rationale for undertaking this study, and for the particular hypothesis?
Response to comment of the reviewerReviewer #1 is quite right, studies to date have shown no compensation in energy intake after accounting for relative energy intake (REI). However, previous studies have not induced such a large energy deficit during a single bout of exercise. Moreover, previous studies have not examined appetite and energy intak...