The role of phosphoenolpyruvate carboxykinase in a marine macroalga with C4-like photosynthetic characteristics.

Reiskind, Julia B.; Bowes, George

doi:10.1073/pnas.88.7.2883

Cited by 117 publications

(75 citation statements)

References 17 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The C 4 syndrome may be constitutive in Ottelia as it is in the marine macroalga Udotea flabellum (Reiskind and Bowes 1991;Reiskind et al 1988) although the effect on Ottelia of other environmental factors such as low temperature or light has not been tested.…”

Section: Kinetics Of O 2 Evolutionmentioning

confidence: 99%

“…The marine macroalga Udotea flabellum (Chlorophyta, Udoteaceae) performs C 4 metabolism but PEPCK is believed to carry out the dual role of carboxylation and decarboxylation (Reiskind and Bowes 1991). It has recently been proposed that another marine macroalga Ulva prolifera (Chlorophyta, Ulvophyceae) has C 4 metabolism based on the presence of PEPC and PPDK (Xu et al 2012).…”

Section: Distribution Of Biochemical Ccms In Terrestrial and Aquatic mentioning

confidence: 99%

See 1 more Smart Citation

Biochemical and biophysical CO2 concentrating mechanisms in two species of freshwater macrophyte within the genus Ottelia (Hydrocharitaceae)

et al. 2013

View full text Add to dashboard Cite

Two freshwater macrophytes, Ottelia alismoides and Ottelia acuminata, were grown at low (mean 5 µmol L -1 ) and high (mean 400 µmol L -1 ) CO 2 concentrations under natural conditions. The ratio of PEPC to RuBisCO activity was 1.8 in O. acuminata in both treatments. In O. alismoides, this ratio was 2.8 and 5.9 when grown at high and low CO 2 , respectively, as a result of a 2-fold increase in PEPC activity. The activity of PPDK was similar to, and changed with, PEPC (1.9-fold change). The activity of the decarboxylating NADP-malic enzyme (ME) was very low in both species while NAD-ME activity was high and increased with PEPC activity in O. alismoides. These results suggest that O.alismoides might perform a type of C 4 metabolism with NAD-ME decarboxylation, despite lacking Kranz anatomy. The C 4 -activity was still present at high CO 2 suggesting that it could be constitutive.O. alismoides at low CO 2 showed diel acidity variation of up to 34 μequiv g -1 FW indicating that it may also operate a form of Crassulacean Acid Metabolism (CAM). pH-drift experiments showed that both species were able to use bicarbonate. In O. acuminata, the kinetics of carbon uptake were altered by CO 2 growth conditions, unlike in O. alismoides. Thus the two species appear to regulate their carbon concentrating mechanisms differently in response to changing CO 2 . O. alismoides is potentially using three different concentrating mechanisms. The Hydrocharitaceae have many species with evidence for C 4 , CAM, or some other metabolism involving organic acids, and are worthy of further study.3

show abstract

Section: Kinetics Of O 2 Evolutionmentioning

confidence: 99%

Section: Distribution Of Biochemical Ccms In Terrestrial and Aquatic mentioning

confidence: 99%

Biochemical and biophysical CO2 concentrating mechanisms in two species of freshwater macrophyte within the genus Ottelia (Hydrocharitaceae)

et al. 2013

View full text Add to dashboard Cite

show abstract

“…These results caused us to suggest a functional model that assumes dark fixation of CO 2 into an internal carbon pool and the release of CO 2 from the pool by the action of blue light and a circadian activity which, in red light, has its maximum during the circadian day. Deprived of the complications of its regulation by circadian rhythmicity and by blue light, the mechanism has similarities to an intracellular C 4 photosynthetic metabolism and, therefore, is reminiscent of the situation reported for the marine green alga Udotea flabellum (Reiskind, Seamon & Bowes 1988Reiskind & Bowes 1991).…”

Section: Introductionmentioning

confidence: 99%

Photosynthesis of Ectocarpus siliculosus in red light and after pulses of blue light at high pH – evidence for bicarbonate uptake

Schmid

1998

Plant Cell & Environment

View full text Add to dashboard Cite

Pulses of blue light cause stimulation of red light saturated photosynthesis in Ectocarpus siliculosus, because blue light activates the operation of a pathway for inorganic carbon (C i ) acquisition by inducing the mobilization of CO 2 from an intermediate metabolite. In the absence of exogenous C i , photosynthetic rates roughly equal those of CO 2 release by respiration. In seawater of pH 9·5 (2·3 mol m -3 total C i , but concentrations of free CO 2 below 0·2 mmol m -3 ), photosynthesis was clearly above these rates, although they were only ≈ 30% of those in normal seawater (≈ pH 8). The degree and the time course of the stimulations of photosynthesis by pulses of blue light were unaltered at high pH. Essentially the same characteristics were found after buffering or in the presence of acetazolamide, an inhibitor of extracellular carbonic anhydrase activity. Therefore, it is concluded that Ectocarpus is able to directly take up HCO 3 -in addition to CO 2 (uptake of CO 3 2-cannot be excluded). The dependence of photosynthesis on C i at pH 9·5 was biphasic, with C i below 0·2 mol m -3 having no effect at all. In C i -free seawater, the shapes of the stimulations after blue light pulses differed for pH 6, pH 8 and pH 9·5. At low pH, only the fast peak (maximum ≈ 5 min after blue light) was detected, whereas at high pH mainly the slow peak (maximum ≈ 20 min after blue light) was observed. At the intermediate pH 8, both peaks were present. As inhibition of total carbonic anhydrase by ethoxyzolamide brought out the fast peak of the stimulations at pH 9·5 it is concluded that the fast component was due to a transient disequilibrium of an intracellular pool of C i which, after blue light, was fed by CO 2 released from the postulated storage intermediate.

show abstract

“…In plants, phosphoenolpyruvate carboxykinase (PEPCK, EC 4.1.1.49) is important in gluconeogenesis during the germination of fat-storing seeds, such as cucumber [1], and it plays a key role in photosynthetic carbon assimilation in one group of C4 plants [2], in some Crassulacean Acid Metabolism (CAM) plants [2], and in the CO2-concentrating mechanism of certain algae [3].…”

Section: Introductionmentioning

confidence: 99%

Purification, and phosphorylation in vivo and in vitro, of phosphoenolpyruvate carboxykinase from cucumber cotyledons

Walker

Leegood

1995

FEBS Letters

View full text Add to dashboard Cite

The role of phosphoenolpyruvate carboxykinase in a marine macroalga with C4-like photosynthetic characteristics.

Abstract: Udotea flabellum is a marine, macroscopic green alga with C4-like photosynthetic characteristics, including little 02 inhibition of photosynthesis, a low CO2 compensation point, and minimal photorespiration; but it lacks anatomical features analogous to the

Cited by 117 publications

References 17 publications

Biochemical and biophysical CO2 concentrating mechanisms in two species of freshwater macrophyte within the genus Ottelia (Hydrocharitaceae)

Biochemical and biophysical CO2 concentrating mechanisms in two species of freshwater macrophyte within the genus Ottelia (Hydrocharitaceae)

Photosynthesis of Ectocarpus siliculosus in red light and after pulses of blue light at high pH – evidence for bicarbonate uptake

Purification, and phosphorylation in vivo and in vitro, of phosphoenolpyruvate carboxykinase from cucumber cotyledons

Contact Info

Product

Resources

About