The Role of Peroxiredoxins in Oxygenic Photosynthesis of Cyanobacteria and Higher Plants: Peroxide Detoxification or Redox Sensing?

Dietz, Karl‐Josef; Stork, Tina; Finkemeier, Iris; Lamkemeyer, Petra; Li, Wenxue; El-Tayeb, Mohamed A.; Michel, Klaus-Peter; Pistorius, Elfriede K.; Baier, Margarete

doi:10.1007/1-4020-3579-9_19

Cited by 14 publications

(12 citation statements)

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“…It could also suggest that the rate of superoxide anion formation was lower in PSII and PSI of Fe-starved cells when the protective proteins IdiA and IsiA were expressed at highly elevated concentrations. Moreover, the transcript pool for the heme-containing catalase peroxidase KatG (Tichy and Vermaas, 1999) was reduced under Fe limitation, while the transcript abundance for aphC (gene 2309), encoding a 2-Cys peroxiredoxin (Tichy and Vermaas, 1999;Dietz et al, 2002;Stork et al, 2005), was increased. This increase only occurred under prolonged Fe limitation.…”

Section: Transcripts Of Genes Encoding General Stress Proteinsmentioning

confidence: 99%

Transcript Profiling Reveals New Insights into the Acclimation of the Mesophilic Fresh-Water CyanobacteriumSynechococcus elongatusPCC 7942 to Iron Starvation

Nodop

Pietsch²,

Höcker³

et al. 2008

Plant Physiology

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The regulatory network for acclimation of the obligate photoautotrophic fresh water cyanobacterium Synechococcus elongatus PCC 7942 to iron (Fe) limitation was studied by transcript profiling with an oligonucleotide whole genome DNA microarray. Six regions on the chromosome with several Fe-regulated genes each were identified. The irpAB and fut region encode putative Fe uptake systems, the suf region participates in [Fe-sulfur] cluster assembly under oxidative stress and Fe limitation, the isiAB region encodes CP43# and flavodoxin, the idiCB region encodes the NuoE-like electron transport associated protein IdiC and the transcriptional activator IdiB, and the ackA/pgam region encodes an acetate kinase and a phosphoglycerate mutase. We also investigated the response of two S. elongatus PCC 7942 mutants to Fe starvation. These were mutant K10, lacking IdiB but containing IdiC, and mutant MuD, representing a idiC-merodiploid mutant with a strongly reduced amount of IdiC as well as IdiB. The absence of IdiB in mutant K10 or the strongly reduced amount of IdiB in mutant MuD allowed for the identification of additional members of the Fe-responsive IdiB regulon. Besides idiA and the irpAB operon somB(1), somA(2), ftr1, ackA, pgam, and nat also seem to be regulated by IdiB. In addition to the reduced amount of IdiB in MuD, the low concentration of IdiC may be responsible for a number of additional changes in the abundance of mainly photosynthesis-related transcripts as compared to the wild type and mutant K10.

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Section: Transcripts Of Genes Encoding General Stress Proteinsmentioning

confidence: 99%

Transcript Profiling Reveals New Insights into the Acclimation of the Mesophilic Fresh-Water CyanobacteriumSynechococcus elongatusPCC 7942 to Iron Starvation

Nodop

Pietsch²,

Höcker³

et al. 2008

Plant Physiology

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“…Analyses of the genome sequence of Synechocystis sp. PCC 6803 revealed the presence of five genes encoding peroxiredoxins 2-Cys PRX ( sll0755 ), 1-Cys PRX ( sll1198 ), two PRX Q ( sll0221 and slr0242 ) and one Type II PRX ( sll1621 ) [19,28,33]. Analyses of the genome sequence of Synechococcus elongatus PCC 7942 led to identification of six putative prx genes including one 1-Cys PRX, one 2-Cys PRX and four PRX Q [34].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of putative peroxiredoxin in unicellular and filamentous cyanobacteria

Cui

Wang

et al. 2012

BMC Evol Biol

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BackgroundCyanobacteria are photoautotrophic prokaryotes with wide variations in genome sizes and ecological habitats. Peroxiredoxin (PRX) is an important protein that plays essential roles in protecting own cells against reactive oxygen species (ROS). PRXs have been identified from mammals, fungi and higher plants. However, knowledge on cyanobacterial PRXs still remains obscure. With the availability of 37 sequenced cyanobacterial genomes, we performed a comprehensive comparative analysis of PRXs and explored their diversity, distribution, domain structure and evolution.ResultsOverall 244 putative prx genes were identified, which were abundant in filamentous diazotrophic cyanobacteria, Acaryochloris marina MBIC 11017, and unicellular cyanobacteria inhabiting freshwater and hot-springs, while poor in all Prochlorococcus and marine Synechococcus strains. Among these putative genes, 25 open reading frames (ORFs) encoding hypothetical proteins were identified as prx gene family members and the others were already annotated as prx genes. All 244 putative PRXs were classified into five major subfamilies (1-Cys, 2-Cys, BCP, PRX5_like, and PRX-like) according to their domain structures. The catalytic motifs of the cyanobacterial PRXs were similar to those of eukaryotic PRXs and highly conserved in all but the PRX-like subfamily. Classical motif (CXXC) of thioredoxin was detected in protein sequences from the PRX-like subfamily. Phylogenetic tree constructed of catalytic domains coincided well with the domain structures of PRXs and the phylogenies based on 16s rRNA.ConclusionsThe distribution of genes encoding PRXs in different unicellular and filamentous cyanobacteria especially those sub-families like PRX-like or 1-Cys PRX correlate with the genome size, eco-physiology, and physiological properties of the organisms. Cyanobacterial and eukaryotic PRXs share similar conserved motifs, indicating that cyanobacteria adopt similar catalytic mechanisms as eukaryotes. All cyanobacterial PRX proteins share highly similar structures, implying that these genes may originate from a common ancestor. In this study, a general framework of the sequence-structure-function connections of the PRXs was revealed, which may facilitate functional investigations of PRXs in various organisms.

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“…Synechocystis PCC 6803 is the cyanobacterium studied best with respect to its antioxidant system. It expresses a superoxide dismutase (slr1516), a catalase peroxidase (sll1987) and Wve peroxiredoxins (Prx) (Kaneko et al 1996;Kobayashi et al 2004;Hosoya-Matsuda et al 2005;Dietz et al 2005;Stork et al 2005). Perelman et al (2003) analysed a double mutant of S. elongatus PCC 7942 deWcient in both the catalase and the 2-Cys Prx homologue.…”

Section: Introductionmentioning

confidence: 99%

Functional characterisation of the peroxiredoxin gene family members of Synechococcus elongatus PCC 7942

et al. 2008

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The genome of Synechococcus elongatus PCC 7942 encodes six peroxiredoxins (Prx). Single genes are present each for a 1-Cys Prx and a 2-Cys Prx, while four genes code for PrxQ-like proteins (prxQ-A1, -A2, -A3 and B). Their transcript accumulation varies with growth conditions in a gene-speciWc manner (Stork et al. in J Exp Bot 56:3193-3206, 2005). To address their functional properties, members of the prx gene family were produced as recombinant proteins and analysed for their peroxide detoxiWcation capacity and quaternary structure by size exclusion chromatography. Independent of the reduction state, the 2-Cys Prx separated as oligomer, the 1-Cys Prx as dimer and the PrxQ-A1 as monomer. PrxQ-A2 was inactive in our assays, 1-Cys Prx activity was unaVected by addition of TrxA, while all others were stimulated to a variable extent by addition of E. coli thioredoxin. Sensitivity towards cumene hydroperoxide treatment of E. coli BL21 cells expressing the cyanobacterial PrxQ-A1 to A3 proteins was greatly reduced, while expression of the other Prx had no eVect. The study shows diVerentiation of Prx functions in S. elongatus PCC 7942 which is discussed in relation to potential roles in site-and stress-speciWc defence.

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The Role of Peroxiredoxins in Oxygenic Photosynthesis of Cyanobacteria and Higher Plants: Peroxide Detoxification or Redox Sensing?

Cited by 14 publications

References 88 publications

Transcript Profiling Reveals New Insights into the Acclimation of the Mesophilic Fresh-Water CyanobacteriumSynechococcus elongatusPCC 7942 to Iron Starvation

Transcript Profiling Reveals New Insights into the Acclimation of the Mesophilic Fresh-Water CyanobacteriumSynechococcus elongatusPCC 7942 to Iron Starvation

Genome-wide analysis of putative peroxiredoxin in unicellular and filamentous cyanobacteria

Functional characterisation of the peroxiredoxin gene family members of Synechococcus elongatus PCC 7942

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