The role of perisynaptic Schwann cells in development of neuromuscular junctions in the frog (xenopus laevis)

Herrera, Albert A.; Qiang, Huahong; Ko, Chien‐Ping

doi:10.1002/1097-4695(200012)45:4<237::aid-neu5>3.0.co;2-j

Cited by 44 publications

(34 citation statements)

References 43 publications

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“…Similar to their counterparts in the CNS, PSCs are also actively involved in multiple aspects of the NMJ (Kang et al, 2003;Koirala et al, 2003;Colomar and Robitaille, 2004;Feng and Ko, 2007;Ko et al, 2007). For example, PSCs extend processes that guide the growth of nerve terminals during development (Herrera et al, 2000;Reddy et al, 2003). These PSC processes are required for the growth and maintenance of developing synapses, because less synaptic growth and more synaptic retraction occur when PSCs are removed by complement-mediated cell lysis .…”

Section: Introductionmentioning

confidence: 99%

Schwann Cells Promote Synaptogenesis at the Neuromuscular Junction via Transforming Growth Factor-β1

Feng¹,

Ko²

2008

J. Neurosci.

130

107

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Section: Introductionmentioning

confidence: 99%

Schwann Cells Promote Synaptogenesis at the Neuromuscular Junction via Transforming Growth Factor-β1

Feng¹,

Ko²

2008

J. Neurosci.

130

107

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“…29,30 Schwann cells play a fundamental role in the regeneration of the peripheral nervous system. Some examples of their roles: migration to the damaged site on both stumps; production of neurotrophic factors; 31 regulation of axonal growth that precedes remyelinization; supply of spatial orientation; 32 organization of the compartments around the axons, by the recruitment of adhesion molecules and enabling the accumulation of protein and the formation of ion channel; 33 driving of axons between and among contiguous synapses and provision of conditions for maturation of the neuromuscular synapses. 34 Among the substances capable of causing stimulation of Schwann cells, those that merit special emphasis are the neuregulins.…”

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confidence: 99%

Neuregulinas 1-alfa e 1-beta na regeneração de nervos periféricos

2010

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OBJETIVO: Avaliar o efeito das neuregulinas 1-alfa e 1-beta na regeneração de nervos ciáticos de camundongos C57BL/6J, adultos, machos, através da técnica de tubulização. MÉTODOS: Utilizaram-se 18 animais, divididos em 3 grupos, implantando-se prótese de polietileno em falhas de 4,0 mm no nervo ciático esquerdo: grupo 1 contendo apenas colágeno purificado (Vitrogen®); grupo 2, colágeno associado a neuregulina 1-alfa; grupo 3 com colágeno e neuregulina 1-beta. O grupo controle foi formado por 6 segmentos de nervos ciáticos direitos. Após 4 semanas, os animais foram sacrificados; extraiu-se segmento do ponto médio do nervo regenerado no interior das próteses, padronizaram-se cortes histológicos e confecção das lâminas para análise histomorfométrica. Confrontaram-se os resultados estatisticamente. RESULTADOS: Os animais tratados com neuregulinas tiveram maior número de axônios mielinizados, com diferença estatisticamente significante quando comparados ao grupo colágeno. Não houve diferença estatística entre os grupos de neuregulinas 1-alfa e 1-beta. CONCLUSÃO: a adição de neuregulinas proporcionou aumento significativo do número de fibras mielinizadas.

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“…Although recent evidence has been obtained at the amphibian NMJ (Herrera et al, 2000;Koirala et al, 2000), most evidence has been obtained at the mammalian NMJ. For instance, processes extended by PSCs after denervation influence the regrowth of motor axons to the muscles and their guidance back to denervated NMJs (Son et al, 1996).…”

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confidence: 99%

Synapse–Glia Interactions at the Mammalian Neuromuscular Junction

2001

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Perisynaptic Schwann cells (PSCs) play critical roles in regulating and stabilizing nerve terminals at the mammalian neuromuscular junction (NMJ). Howe¹er, although these functions are likely regulated by the synaptic properties, the interactions of PSCs with the synaptic elements are not known. Therefore, our goal was to study the interactions between mammalian PSCs in situ and the presynaptic terminals using changes in intracellular Ca 2ϩ as an indicator of cell activity. Motor nerve stimulation induced an increase in intracellular Ca 2ϩ in PSCs, and this increase ÿas greatly reduced when transmitter release was blocked. Furthermore, local application of acetylcholine induced Ca 2ϩ responses that were blocked by the muscarinic antagonist atropine and mimicked by the muscarinic agonist muscarine. The nicotinic antagonist ␣-bungarotoxin had no effect on Ca 2ϩ responses induced by acetylcholine. Local application of the cotransmitter ATP induced Ca 2ϩ responses that were unaffected by the P2 antagonist suramin, whereas local application of adenosine induced Ca 2ϩ responses that were greatly reduced by the A1 receptor antagonist 8-cyclopentyl-1,3-dimethylxanthine (CPT). However, the presence of the A1 antagonist in the perfusate did not block responses induced by ATP. Ca 2ϩ responses evoked by stimulation of the motor nerve were reduced in the presence of CPT, whereas atropine almost completely abolished them. Ca 2ϩ responses were further reduced when both antagonists were present simultaneously. Hence, PSCs at the mammalian NMJ respond to the release of neurotransmitter induced by stimulation of the motor nerve through the activation of muscarinic and adenosine A1 receptors.

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The role of perisynaptic Schwann cells in development of neuromuscular junctions in the frog (xenopus laevis)

Cited by 44 publications

References 43 publications

Schwann Cells Promote Synaptogenesis at the Neuromuscular Junction via Transforming Growth Factor-β1

Schwann Cells Promote Synaptogenesis at the Neuromuscular Junction via Transforming Growth Factor-β1

Neuregulinas 1-alfa e 1-beta na regeneração de nervos periféricos

Synapse–Glia Interactions at the Mammalian Neuromuscular Junction

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