The role of particle-mediated DNA vaccines in biodefense preparedness

Dean, Hansi; Haynes, Joel R.; Schmaljohn, Connie S.

doi:10.1016/j.addr.2005.01.012

Cited by 38 publications

(28 citation statements)

References 198 publications

(231 reference statements)

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“…Nonetheless, it is reassuring to see that in spite of all of the different manipulations and techniques used, many epitopes were independently identified by more than one group. Examples include: C7L [74][75][76][77][78][79][80][81][82] found by four separate groups, D5R 349-357 identified by both Oseroff and Jing, G5R [18][19][20][21][22][23][24][25][26] by three groups, both H3L 184-192 and I3L [116][117][118][119][120][121][122][123][124] found by Oseroff and Pasquetto, and J6R 303-311 identified by both Oseroff and Terajima. Another excellent example is the fact that using a consensus epitope approach Moutaftsi et al confirmed all five mouse epitopes that their group had previously identified using an expression library approach [33,106].…”

Section: Poxvirus Epitope Discoverymentioning

confidence: 98%

T‐Cell epitope discovery for variola and vaccinia viruses

Kennedy

2006

Reviews in Medical Virology

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Variola major, the causative agent of smallpox, afflicted mankind throughout history until the worldwide World Health Organisation WHO vaccination campaign successfully eradicated the disease. Unfortunately, recent concerns about bioterrorism have renewed scientific interest in this virus. One essential component of our biodefense and preparedness efforts is an understanding of poxvirus immunity. To this end a number of laboratories have sought to discover T- and B-Cell epitopes from select agents such as variola virus. This review focuses on the efforts to identify CD8(+) T-Cell epitopes from poxviruses as a means to develop new vaccines and therapeutics. A wide variety of techniques have been employed by several research groups to provide complementary information regarding cellular immune responses to poxviruses. In the last several years well over 100 T-Cell epitopes have been identified and the work rapidly continues. The information gleaned from these studies will not only give us a greater understanding of immunity to variola virus and other viruses, but also provide a foundation for next generation vaccines and additional tools with which to study host-pathogen interactions.

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Section: Poxvirus Epitope Discoverymentioning

confidence: 98%

T‐Cell epitope discovery for variola and vaccinia viruses

Kennedy

2006

Reviews in Medical Virology

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“…Gold nanoparticles have been studied as potential vaccine carriers and in transdermal delivery (15)(16)(17)(18). Gold facial masks are now being used in beauty clinics and saloons.…”

Section: Introductionmentioning

confidence: 99%

Novel Vitamin and Gold-Loaded Nanofiber Facial Mask for Topical Delivery

et al. 2010

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Abstract. L-ascorbic acid has been widely used in cosmetic and dermatological products because of its ability to scavenge free radicals and destroy oxidizing agents. However, it is chemically unstable and can easily be oxidized. The current cosmetic facial masks available in the market are pre-moistened, which means that the aqueous fluid content of the mask may oxidize some of the unstable active ingredients such as ascorbic acid. This work presents an anti-wrinkle nanofiber face mask containing ascorbic acid, retinoic acid, gold nanoparticles, and collagen. This novel face mask will only be wetted when applied to the skin, thus enhancing product stability. Once moistened, the content of the mask will gradually dissolve and release the active ingredients and ensure maximum skin penetration. The high surface areato-volume ratio of the nanofiber mask will ensure maximum contact with the skin surface and help to enhance the skin permeation to restore its healthy appearance. Electrospun fiber mats may provide an attractive alternative to the commercial facial cotton masks.

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“…Immune responses in larger species elicited by intramuscular injection of pDNA have been generally lower than those elicited in small animals, with 1000-fold more pDNA required for effective immunization of larger species than of smaller species (mg versus mg) (Kutzler & Weiner, 2008). However, PMED of DNA vaccines enables significant responses in microgram doses in nonhuman primates (NHPs) (Dean et al, 2005;Fuller et al, 2006Fuller et al, , 2002Payne, Fuller, & Haynes, 2002). In humans, PMED delivery of pDNA was demonstrated to be safe (Payne et al, 2002;Tacket et al, 1999).…”

Section: Particle-mediated Epidermal Delivery or "Gene Gun"mentioning

confidence: 95%

“…In the skin, nucleic acid is delivered directly into the cytoplasm or nuclei of keratinocytes and Langerhans cells at the site of administration (Fuller, Loudon, & Schmaljohn, 2006). PMED delivery systems have been employed since the mid-1980s for the direct delivery of pDNA for gene therapy and vaccine applications (For a review, see (Dean, Haynes, & Schmaljohn, 2005;Fuller et al, 2006). Immune responses in larger species elicited by intramuscular injection of pDNA have been generally lower than those elicited in small animals, with 1000-fold more pDNA required for effective immunization of larger species than of smaller species (mg versus mg) (Kutzler & Weiner, 2008).…”

Section: Particle-mediated Epidermal Delivery or "Gene Gun"mentioning

confidence: 99%