The role of P2X3 receptors in bilateral masseter muscle allodynia in rats

Knežević, Petra Tariba; Vukman, Robert; Robert, Aymeric; Kovač, Zoran; Uhač, Ivone; Simonić-Kocijan, Sunčana

doi:10.3325/cmj.2016.57.530

Cited by 10 publications

(17 citation statements)

References 45 publications

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“…The animal characteristics, housing protocols, and treatment protocols are described in detail in our previous study. 24 Briefly, adult male rats weighing 250-300g were housed in groups of two to three per cage under a 12h/12h light/dark cycle. The rats were housed in a controlled temperature (22±2°C) and humidity (55±10%) environment with food and water access ad libitum.…”

Section: Experimental Animalsmentioning

confidence: 99%

“…MM inflammation was induced by unilateral injection of complete Freund's adjuvant (CFA, Sigma F5881, Sigma-Aldrich, Saint Louis, MO, USA; 0.5 mg/mL, heat-killed Mycobacterium tuberculosis suspended in 1:1 oil: saline emulsion), as reported in previous studies. [24][25][26] The protocol details are described in our previous study. 24 Briefly, rats were anesthetized with 4% isoflurane (Forane, Abbott Laboratories Ltd, Queenborough, UK) in a gas mixture O 2 :N 2 =1:2 to abolish righting and corneal reflexes.…”

Section: Establishment Of Bilateral Hypersensitivity Following Unilatmentioning

confidence: 99%

“…[24][25][26] The protocol details are described in our previous study. 24 Briefly, rats were anesthetized with 4% isoflurane (Forane, Abbott Laboratories Ltd, Queenborough, UK) in a gas mixture O 2 :N 2 =1:2 to abolish righting and corneal reflexes. They were then injected with 50 μL of CFA (experimental group) or 0.9% saline (control group) in the right MM mid-region.…”

Section: Establishment Of Bilateral Hypersensitivity Following Unilatmentioning

confidence: 99%

“…[25][26][27][28] The method details are reported in our previous study. 24 Briefly, a von Frey anesthesiometer (VFA; type 2391, IITC Inc., Woodland Hills, CA, USA) was used bilaterally at different time points to establish the nocifensive response. The lowest force needed to cause active head withdrawal from the 1.0 mm probing tip was considered to be the HWT.…”

Section: Mechanical Nocifensive Behavior Measurementsmentioning

confidence: 99%

“…The histopathological MM tissue analysis was performed as reported in our previous study. 24 Briefly, the entire both right and left MMs were fixed in 4% paraformaldehyde, embedded in paraffin, cut at 40 μm intervals, and stained with hematoxylin and eosin.…”

Section: Tissue Isolation Preparation and Analysismentioning

confidence: 99%

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P2Y2 Receptors Mediate Masseter Muscle Mechanical Hypersensitivity in Rats

Knežević

Vukman

Uhač

et al. 2020

JPR

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Purpose: P 2 Y 2 receptors (P 2 Y 2 Rs) are among the various receptors that play an important role in nociception. The goal of this research was to investigate possible P 2 Y 2 R expression changes in the trigeminal ganglion (TRG) in bilateral masseter muscle (MM) hypersensitivity following unilateral MM inflammation. The impact of unilateral intramasseteric administration of P 2 Y 2 R antagonist on bilateral MM hypersensitivity was also explored. Materials and Methods: Bilateral MM hypersensitivity was provoked by unilateral intramasseteric injection of complete Freund's adjuvant (CFA). The head withdrawal threshold (HWT) was assessed bilaterally 4 days later. Bilateral TRG and MM isolation were followed, and quantitative real-time polymerase chain reaction (qRT-PCR) and histopathological analysis were carried out on these tissues, respectively. The involvement of P 2 Y 2 Rs in nocifensive behavior was evaluated by administering two doses of P 2 Y 2 R antagonist AR-C118925 (0.2 or 1 mg/100 μL) in inflamed MM 4 days post-CFA administration. Bilateral HWT was assessed at different time points following antagonist injection. Results: qRT-PCR analysis demonstrated P 2 Y 2 R up-regulation in TRG ipsilateral to the site of CFA administration. Compared to the controls, both doses of AR-C118925 injected ipsilateral to the TRG increased the bilateral HWT at 30, 60, 90, and 120 minutes after antagonist administration. Conclusion: The findings suggest that P 2 Y 2 Rs may affect MM inflammatory hypersensitivity owing to its up-regulation in the TRG in MM inflammatory pain states.

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Section: Experimental Animalsmentioning

confidence: 99%

Section: Establishment Of Bilateral Hypersensitivity Following Unilatmentioning

confidence: 99%

Section: Establishment Of Bilateral Hypersensitivity Following Unilatmentioning

confidence: 99%

Section: Mechanical Nocifensive Behavior Measurementsmentioning

confidence: 99%

Section: Tissue Isolation Preparation and Analysismentioning

confidence: 99%

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P2Y2 Receptors Mediate Masseter Muscle Mechanical Hypersensitivity in Rats

Knežević

Vukman

Uhač

et al. 2020

JPR

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P2X3 receptors contribute to muscle pain induced by static contraction by a mechanism dependent on neutrophil migration

Aquino

Santos

Jorge

et al. 2019

Purinergic Signalling

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P2X3 receptors are involved with several pain conditions. Muscle pain induced by static contraction has an important socioeconomic impact. Here, we evaluated the involvement of P2X3 receptors on mechanical muscle hyperalgesia and neutrophil migration induced by static contraction in rats. Also, we evaluated whether static contraction would be able to increase muscle levels of TNF-α and IL-1β. Male Wistar rats were pretreated with the selective P2X3 receptor antagonist, A-317491, by intramuscular or intrathecal injection and the static contraction-induced mechanical muscle hyperalgesia was evaluated using the Randall-Selitto test. Neutrophil migration was evaluated by measurement of myeloperoxidase (MPO) kinetic-colorimetric assay and the cytokines TNF-α and IL-1β by enzyme-linked immunosorbent assay. Intramuscular or intrathecal pretreatment with A-317491 prevented static contraction-induced mechanical muscle hyperalgesia. In addition, A-317491 reduced static contraction-induced mechanical muscle hyperalgesia when administered 30 and 60 min of the beginning of static contraction, but not after 30 and 60 min of the end of static contraction. Intramuscular A-317491 also prevented static contraction-induced neutrophil migration. In a period of 24 h, static contraction did not increase muscle levels of TNF-α and IL-1β. These findings demonstrated that mechanical muscle hyperalgesia and neutrophil migration induced by static contraction are modulated by P2X3 receptors expressed on the gastrocnemius muscle and spinal cord dorsal horn. Also, we suggest that P2X3 receptors are important to the development but not to maintenance of muscle hyperalgesia. Therefore, P2X3 receptors can be pointed out as a target to musculoskeletal pain conditions induced by daily or work-related activities.

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Association between P2X3 receptors and neuropathic pain: As a potential therapeutic target for therapy

Dong

Zhang

Luo

2022

Biomedicine & Pharmacotherapy

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The role of P2X3 receptors in bilateral masseter muscle allodynia in rats

Cited by 10 publications

References 45 publications

<p>P<sub>2</sub>Y<sub>2</sub> Receptors Mediate Masseter Muscle Mechanical Hypersensitivity in Rats</p>

<p>P<sub>2</sub>Y<sub>2</sub> Receptors Mediate Masseter Muscle Mechanical Hypersensitivity in Rats</p>

P2X3 receptors contribute to muscle pain induced by static contraction by a mechanism dependent on neutrophil migration

Association between P2X3 receptors and neuropathic pain: As a potential therapeutic target for therapy

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