The Role of Oxygen in Cutaneous Photodynamic Therapy

Fuchs, Jürgen; Thiele, Jens J.

doi:10.1016/s0891-5849(97)00370-5

Cited by 170 publications

(137 citation statements)

References 120 publications

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“…Singlet oxygen ("O # ) is a well-recognized product of many photochemical reactions [32] and has been shown previously to oxidize DMPO to DMPO\dOH [33][34][35].…”

Section: Role Of 1 O 2 In Dmpo/doh Formation Resulting From Menadionementioning

confidence: 99%

Biological effects of menadione photochemistry: effects of menadione on biological systems may not involve classical oxidant production

McCormick

Denning

Reszka

et al. 2000

Biochemical Journal

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Because cell-mediated reduction of menadione leads to the generation of reactive oxygen species (ROS), this quinone is widely used to investigate the effects of ROS on cellular functions. We report that A549 human lung epithelial cells exposed to menadione demonstrate a dose-dependent increase in both intracellular calcium ([Ca# + ] i ) and ROS formation. The concentrations of menadione required to initiate these two events are markedly different, with ROS detection requiring higher levels of menadione. Modulators of antioxidant defences (e.g. buthionine sulphoximine, 3-amino-1,2,4-triazole) have little effect on the [Ca# + ] i response to menadione, suggesting that ROS formation does not account for menadione-dependent alterations in [Ca# + ] i . Additional evidence suggests that menadione photochemistry may be responsible for the observed [Ca# + ] i effects. Specifically : (a) EPR studies with the spin trap 5,5-dimethyl-1-pyrroline-Noxide (DMPO) show that light exposure (maximum effect at

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“…Singlet oxygen ("O # ) is a well-recognized product of many photochemical reactions [32] and has been shown previously to oxidize DMPO to DMPO\dOH [33][34][35].…”

Section: Role Of 1 O 2 In Dmpo/doh Formation Resulting From Menadionementioning

confidence: 99%

Biological effects of menadione photochemistry: effects of menadione on biological systems may not involve classical oxidant production

McCormick

Denning

Reszka

et al. 2000

Biochemical Journal

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“…This is supported by the fact that the PDT effects are decreased or abolished, both in vivo and in vitro, at low oxygen concentrations (14)(15)(16). There is evidence that, during PDT light irradiation, oxygen levels within the tumor are affected by the production of reactive oxygen species and PDT-induced vascular damage (17).…”

Section: Enhancing the Photodynamic Effect Of Hypericin In Human Bladmentioning

confidence: 97%

Enhancing the photodynamic effect of hypericin in human bladder transitional cell carcinoma spheroids by the use of the oxygen carrier, perfluorodecalin

Kamuhabwa¹,

Huygens²,

Roskams³

et al. 2006

Int J Oncol

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Abstract. In the present study, we evaluated the possibility of enhancing the photodynamic effect of hypericin in transitional cell carcinoma (TCC) spheroids by the use of the oxygen carrier, perfluorodecalin. Following incubation with hypericin, RT-112 TCC spheroids were irradiated in the presence or absence of perfluorodecalin, at light doses of 7 J/cm 2 or 28 J/ cm 2 , delivered at a fluence rate of 15 mW/cm 2 . The photodynamic therapy (PDT) efficacy was evaluated and apoptotic cells were visualized. The results show that, in the absence of perfluorodecalin, spheroidal TCC cells are inadequately sensitive to hypericin PDT. As was shown by fluorescence microscopy, this lack of activity was not due to insufficient photoactive concentrations of hypericin reaching the inner parts of the spheroids. Conversely, enhanced oxygenation of spheroids by perfluorodecalin led to a dramatic enhancement of hypericin PDT efficacy. The detection of nuclear shrinkage or fragmentation with DAPI staining and the assessment of cell morphology by light microscopy indicated that apoptosis was the most prominent response of spheroids to hypericin PDT in the presence of perfluorodecalin. In conclusion, the results of this study suggest that perfluorocarbons, such as perfluorodecalin, are useful in enhancing the oxygenation of tumor tissue, resulting in highly efficient hypericin PDT. Since hypericin becomes concentrated specifically in human bladder urothelial carcinoma lesions and the bladder is very well suited to instillation with a perfluorocarbon, combining the techniques looks very promising for an efficient and selective whole bladder wall photodynamic antitumoral treatment in a urological clinical setting.

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“…This group hypothesised that hyperoxygenation oxygenated preexisting hypoxic cells and compensated for oxygen depletion during PDT [9]. Nevertheless, an obvious disadvantage of HBO is the practical feasibility [11] as a custom-built chamber is required and the authors also highlighted that if a hypoxic region is too far away for oxygen diffusion to take effect then improvement may be limited [9].…”

Section: Discussionmentioning

confidence: 99%

“…Other strategies to increase PDT sensitivity by modulating skin oxygen tension include hyperthermia, and perfluorocarbons [11]. This in vitro study investigated how differences in the oxygen environment, prior to and following the administration of the PpIX precursors aminolevulinic acid (ALA), methyl aminolevulinate (MAL) or hexyl aminolevulinate (HAL) with or without the iron chelator 1,2-diethyl-3-hydroxypyridin-4-one hydrochloride (CP94), affected the PpIX fluorescence produced within human squamous epithelial carcinoma cells.…”

Section: Introductionmentioning

confidence: 99%

The effects of protoporphyrin IX-induced photodynamic therapy with and without iron chelation on human squamous carcinoma cells cultured under normoxic, hypoxic and hyperoxic conditions

Blake

Allen

Curnow

2013

Photodiagnosis and Photodynamic Therapy

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The Role of Oxygen in Cutaneous Photodynamic Therapy

Cited by 170 publications

References 120 publications

Biological effects of menadione photochemistry: effects of menadione on biological systems may not involve classical oxidant production

Biological effects of menadione photochemistry: effects of menadione on biological systems may not involve classical oxidant production

Enhancing the photodynamic effect of hypericin in human bladder transitional cell carcinoma spheroids by the use of the oxygen carrier, perfluorodecalin

The effects of protoporphyrin IX-induced photodynamic therapy with and without iron chelation on human squamous carcinoma cells cultured under normoxic, hypoxic and hyperoxic conditions

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