The role of oxidative stress in fluoride toxicity

Miranda, Giza Hellen Nonato; Ferreira, Maria Karolina Martins; Bittencourt, Leonardo Oliveira; Lima, Leidiane Alencar de Oliveira; Puty, Bruna; Lima, Rafael Rodrigues

doi:10.1016/b978-0-12-819092-0.00017-0

Cited by 7 publications

(3 citation statements)

References 41 publications

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“…In preventive and conservative treatments, fluoride is used as a reference mineral, as it strengthens tooth enamel and helps prevent dental caries [ 9 ]. However, excessive fluoride consumption can cause health problems [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. Fluoride toxicity usually occurs when extreme amounts of fluoride are ingested through drinking water [ 19 , 21 ], fluoride supplements [ 30 , 31 ], toothpaste [ 32 ], or in extreme cases, by ingesting products such as pesticides or insecticides that contain fluoride [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition to dental fluorosis, chronic exposure to high levels of fluoride can cause other adverse health effects [ 15 , 16 , 17 , 18 , 23 , 31 ], such as generating bone problems [ 15 ], causing liver and kidney damage [ 16 ], affecting the gastrointestinal system [ 40 ], and inducing neurological disorders [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 31 , 32 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Ilex guayusa and Piper marginatum Extract Cytotoxicity on Human Dental Pulp Mesenchymal Stem Cells

Sequeda-Castañeda,

Suárez-Carvajal,

Téllez-Corral

et al. 2024

Dentistry Journal

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Background: Amelogenesis imperfecta is a hereditary disorder affecting dental enamel. Among its phenotypes, hypocalcified AI is characterized by mineral deficiency, leading to tissue wear and, consequently, dental sensitivity. Excessive fluoride intake (through drinking water, fluoride supplements, toothpaste, or by ingesting products such as pesticides or insecticides) can lead to a condition known as dental fluorosis, which manifests as stains and teeth discoloration affecting their structure. Our recent studies have shown that extracts from Colombian native plants, Ilex guayusa and Piper marginatum, deposit mineral ions such as phosphate and orthophosphate into the dental enamel structure; however, it is unknown whether these extracts produce toxic effects on the dental pulp. Objective: To assess cytotoxicity effects on human dental pulp stem cells (hDPSCs) exposed to extracts isolated from I. guayusa and P. marginatum and, hence, their safety for clinical use. Methods: Raman spectroscopy, fluorescence microscopy, and flow cytometry techniques were employed. For Raman spectroscopy, hDPSCs were seeded onto nanobiochips designed to provide surface-enhanced Raman spectroscopy (SERS effect), which enhances their Raman signal by several orders of magnitude. After eight days in culture, I. guayusa and P. marginatum extracts at different concentrations (10, 50, and 100 ppm) were added. Raman measurements were performed at 0, 12, and 24 h following extract application. Fluorescence microscopy was conducted using an OLIMPUS fv1000 microscope, a live–dead assay was performed using a kit employing a BD FACS Canto TM II flow cytometer, and data analysis was determined using a FlowJo program. Results: The Raman spectroscopy results showed spectra consistent with viable cells. These findings were corroborated using fluorescence microscopy and flow cytometry techniques, confirming high cellular viability. Conclusions: The analyzed extracts exhibited low cytotoxicity, suggesting that they could be safely applied on enamel for remineralization purposes. The use of nanobiochips for SERS effect improved the cell viability assessment.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of Ilex guayusa and Piper marginatum Extract Cytotoxicity on Human Dental Pulp Mesenchymal Stem Cells

Sequeda-Castañeda,

Suárez-Carvajal,

Téllez-Corral

et al. 2024

Dentistry Journal

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“…Fluorine is a non-metallic element with high electronegativity, which can be found naturally in the forms of fluoride (F). The exposure to this ion can occur both naturally (through volcanic activity, coal combustion, dissolution of minerals, and marine aerosols) and anthropogenically (when F is released as waste from industrial activity or from manufactured products) [1,2]. Due to its unquestionable effects on caries prevention and control, F is also incorporated into vehicles available at a population level (water, salt, milk, sugar, and supplements), as well as at an individual level for professional (gels, foams, and varnishes) and home application (dentifrices and mouthwashes), which are known to contribute to the overall systemic exposure to fluoridated compounds [3].…”

Section: Introductionmentioning

confidence: 99%

Fluoride Exposure and Salivary Glands: How Is Glandular Morphology Susceptible to Long-Term Exposure? A Preclinical Study

Matos-Sousa

Bittencourt

Ferreira

et al. 2022

JCM

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Despite a strong body of evidence attesting to the effectiveness of fluoride (F) in preventing and controlling caries, some studies have sought to investigate the influence of F exposure on the salivary glands, organs that are essential for the maintenance of cavity homeostasis through salivary production, finding that exposure to F can cause biochemical and proteomic changes. Thus, this study aimed to investigate the morphological effects of prolonged exposure to F on the salivary glands of mice, at concentrations that would correspond to optimally fluoridated water (suitable for human consumption) and to fluorosis-endemic regions. Twenty-four male mice (Mus musculus) were divided into three groups, according to F levels in the drinking water: 0 (control), 10, or 50 mg F/L, with an exposure period of 60 days. The glands were morphometrically analyzed for the total acinar area, parenchyma area, and stromal area, as well as for the immunohistochemical analysis of myoepithelial cells. The results showed that prolonged exposure to F at 10 mg F/L did not promote significant changes in the morphometry of the salivary glands of mice, which reinforces the safety of the chronic use of F in low doses.

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