The role of oxidative phosphorylation in the generation of ATP in human spermatozoa

Ford, W. C. L.; Harrison, Anne

doi:10.1530/jrf.0.0630271

Cited by 59 publications

(42 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In fact, mammalian sperm require metabolic energy for a variety of functions (Williams & Ford 2001, Ferramosca & Zara 2014, and it is known that human spermatozoa obtain a significant proportion of their energy from glycolysis (Williams & Ford 2001). Nevertheless, the origin of ATP for sperm motility is still being intensely discussed (Ford & Harrison 1981, Williams & Ford 2001, Ruiz-Pesini et al 2007, Bucci et al 2011, Piomboni et al 2012.…”

Section: Effects Of Substrate Availability On Spermatozoa Functionalitymentioning

confidence: 99%

High glucose concentrations per se do not adversely affect human sperm function in vitro

et al. 2015

View full text Add to dashboard Cite

Diabetes mellitus (DM) represents one of the greatest concerns to global health and it is associated with diverse clinical complications, including reproductive dysfunction. Given the multifactorial nature of DM, the mechanisms that underlie reproductive dysfunction remain unclear. Considering that hyperglycemia has been described as a major effector of the disease pathophysiology, we used an in vitro approach to address the isolated effect of high glucose conditions on human sperm function, thus avoiding other in vivo confounding players. We performed a complete and integrated analysis by measuring a variety of important indicators of spermatozoa functionality (such as motility, viability, capacitation status, acrosomal integrity, mitochondrial superoxide production and membrane potential) in human sperm samples after incubation with D-and L-glucose (5, 25, or 50 mM) for 24 and 48 h. No direct effects promoted by 25 or 50 mM D-glucose were found for any of the parameters assessed (PO0.05), except for the acrosome reaction, which was potentiated after 48 h of exposure to 50 mM D-glucose (P!0.05). Interestingly, non-metabolizable L-glucose drastically increased superoxide production (P!0.05) and suppressed sperm motility (P!0.05) and capacitation (P!0.05) after 24 h of treatment, whereas mitochondrial membrane potential (P!0.05), acrosomal integrity (P!0.01) and viability (P!0.05) were later decreased. The overall results suggest that high glucose levels per se do not influence human sperm function in vitro, which stresses the importance of other factors involved in DM pathology. Nevertheless, the absence of metabolizable glucose contributes to a severe impairment of sperm function and thus compromises male fertility. Free Portuguese abstract: A Portuguese translation of this abstract is freely available at

show abstract

Section: Effects Of Substrate Availability On Spermatozoa Functionalitymentioning

confidence: 99%

High glucose concentrations per se do not adversely affect human sperm function in vitro

et al. 2015

View full text Add to dashboard Cite

show abstract

“…In humans, the exposure of spermatozoa to an ELF-EMF with a square waveform of 5 mT amplitude and frequency of 50 Hz resulted in an increase in motility and kinematic parameters [Iorio et al, 2007]. mitochondrial activity correlates with sperm motility [Gopalkrishnan et al, 1995] and that oxygen consumption is associated with the flagellar movement in human and bull sperm [Ford and Harrison, 1981;Halangk et al, 1990]. Moreover, in human sperm, the functional status of mitochondria is related to sperm motility since high values of mitochondrial membrane potential ðDC high m Þ are correlated with high values of kinematic parameters [Marchetti et al, 2004;Gallon et al, 2006].…”

Section: Introductionmentioning

confidence: 99%

“…The relevance of OXPHOS to sperm motility has also been suggested by studies regarding the effect of specific inhibitors of the respiratory complexes on sperm motility. In particular, rotenone, an inhibitor of respiratory complex I, and antimycina A, an inhibitor of respiratory complex III, depleted ATP and negatively affected sperm motility [Ford and Harrison, 1981;Halangk et al, 1985;de Lamirande and Gagnon, 1992;Ruiz-Pesini et al, 2000].…”

Section: Introductionmentioning

confidence: 99%

Involvement of mitochondrial activity in mediating ELF‐EMF stimulatory effect on human sperm motility

Iorio

Monache

Bennato

et al. 2010

Bioelectromagnetics

View full text Add to dashboard Cite

It has recently been reported that the exposure of human spermatozoa to an extremely low frequency (ELF) electromagnetic field (EMF) with a square waveform of 5 mT amplitude and frequency of 50 Hz improves sperm motility. The functional relationship between the energy metabolism and the enhancement of human sperm motility induced by ELF-EMF was investigated. Sperm exposure to ELF-EMF resulted in a progressive and significant increase of mitochondrial membrane potential and levels of ATP, ADP and NAD(+) that was associated with a progressive and significant increase in the sperm kinematic parameters. No significant effects were detected on other parameters such as ATP/ADP ratio and energy charge. When carbamoyl cyanide m-chlorophenylhydrazone (CICCP) was applied to inhibit the oxidative phosphorylation in the mitochondria, the values of energy parameters and motility in the sperm incubated in the presence of glucose and exposed to ELF-EMF did not change, thus indicating that the glycolysis was not involved in mediating ELF-EMF stimulatory effect on motility. By contrast, when pyruvate and lactate were provided instead of glucose, the energy status and motility increased significantly in ELF-EMF-treated sperm. Under these culture conditions, the inhibition of glycolitic metabolism by 2-deoxy-D-glucose (DOG) again resulted in increased values of energy and kinematic parameters, indicating that gluconeogenesis was not involved in producing glucose for use in glycolysis. We concluded that the key role in mediating the stimulatory effects exerted by ELF-EMF on human sperm motility is played by mitochondrial oxidative phosphorylation rather than glycolysis.

show abstract

“…Although a number of respiratory substrates can be used by spermatozoa, those from many species (including the boar) exhibit a high rate of fructolysis and their energy demands can be fulfilled by substrate-level phosphorylation (Ford and Harrison 1981;Mann and Lutwak-Mann 1981). This is confirmed by the decrease in the energy charge potential of boar spermatozoa when they are incubated in the presence of (S)-cx-chlorohydrin.…”

Section: Discussionmentioning

confidence: 96%

Inhibition of Fructolysis in Boar Spermatozoa by the Male Antifertility Agent (S)-a-Chlorohydrin

Stevenson¹,

Jones²

1982

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

The (S)-isomer of the male antifertility agent IX-chlorohydrin strongly inhibited the oxidative metabolism of fructose by boar spermatozoa in vitro. The result of this action, which has been deduced to be an inhibition of glyceraldehydephosphate dehydrogenase, caused an accumulation of fructose-1,6-bisphosphate and the triosephosphates, and a decrease in substrate-level phosphorylation with a concomitant lowering of the energy charge potential of the spermatozoa. The (R)-isomer of IX-chlorohydrin had no inhibitory activity on fructolysis.A study of the comparative metabolism of (R)-[3-36CI]-IX-chlorohydrin and (R,S)-[3-36Cl]_IX_ chlorohydrin by boar spermatozoa showed that it is the (S)-isomer that specifically undergoes a process of oxidative metabolism to (R)-3-chlorolactaldehyde.It is proposed that this endogenous oxidation product, which has the same absolute configuration as the substrate for glyceraldehydephosphate dehydrogenase, is the active metabolite of (S)-IX-chlorohydrin that inhibits this enzyme. Exogenous (R,S)-3-chlorolactaIdehyde inhibited the oxidative metabolism of fructose by boar spermatozoa, apparently by a mechanism similar to that of (S)-IX-chlorohydrin.

show abstract

The role of oxidative phosphorylation in the generation of ATP in human spermatozoa

Cited by 59 publications

References 35 publications

High glucose concentrations per se do not adversely affect human sperm function in vitro

High glucose concentrations per se do not adversely affect human sperm function in vitro

Involvement of mitochondrial activity in mediating ELF‐EMF stimulatory effect on human sperm motility

Inhibition of Fructolysis in Boar Spermatozoa by the Male Antifertility Agent (S)-a-Chlorohydrin

Contact Info

Product

Resources

About