The Role of Oxidative and Nitrosative Stress of Silver Nanoparticles in Human Parasitic Helminth Brugia malayi: A Mechanistic Insight

Hande, Sneha; Sonkar, Vijay K.; Bhoj, Priyanka; Togre, Namdev; Goswami, Kalyan; Dash, Debabrata

doi:10.1007/s11686-021-00394-4

Cited by 5 publications

(2 citation statements)

References 40 publications

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“…Additionally, it is worth to note that, the synthesized polymers showed a hemolytic rate of <5% (Table S2, Supporting Information), indicating their nontoxic nature. [ 56 ] Figure 4B represents the histogram of the hemolytic rate of different concentrations of antioxidant polymers.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Polymers with Enhanced Neuroprotection Against Insulin Fibrillation

Bera

Ghosh

et al. 2023

Macromolecular Bioscience

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Lipoic acid (LA) and dihydrolipoic acid (DHLA) are well established antioxidants to scavenge reactive oxygen species (ROS). However, they are carboxylates with ≈4.7 pKa making them negatively charged at physiological pH (7.4) reducing their passive diffusion through cell membranes. LA is known to be capable of reducing protein fibrillation. Incorporation of LA and especially DHLA in polymer side chains are scarce. Herein, the first examples of the anti‐amyloidogenic effect of LA and DHLA incorporated into the side‐chain of a block copolymer with a water‐soluble poly(polyethylene glycol methyl ether methacrylate) (PPEGMA) segment are presented. The resultant polymers show improved ROS scavenging activity and improved ability to reduce insulin fibrillation compared to free LA and DHLA. Furthermore, the resultant polymers are also capable of disintegrating preformed insulin firbrils. Interestingly, polymers with dihydro‐lipoate moieties showed 93% free radical scavenging activity with 91% anti‐fibrillating efficacies for insulin protein confirmed by 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assay and Thioflavin T (ThT) dye binding study, respectively. Further, the antioxidant polymers increase the cell viability against fibrillar insulin aggregates that may be involved in the etiology of several diseases. Overall, this work reveals that antioxidant polymer‐based therapeutic agents can serve as a powerful modulation strategy for developing novel drugs in future against amyloid‐related disorders.

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Section: Resultsmentioning

confidence: 99%

Antioxidant Polymers with Enhanced Neuroprotection Against Insulin Fibrillation

Bera

Ghosh

et al. 2023

Macromolecular Bioscience

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“…The hemolytic percentages of Ag NPs, PAla-AgNPs , PLeu-AgNPs , and PPhe-AgNPs were found to be 3.2, 2.3, 1.4, and 0.8%, respectively. If the hemolytic percentage is less than 5%, samples are regarded as nonhemolytic, according to ISO/TR 7405-1984(f). , Samples with 5–10% hemolytic percentage are considered to be slightly hemolytic, whereas greater than 10% hemolytic value is considered to be very hemolytic. In our cases, all of the PC-AgNPs showed a hemolytic percentage less than 5% (Figure S9), demonstrating that the NPs are nonhemolytic.…”

Section: Resultsmentioning

confidence: 99%

Amino Acid-Based Polymer-Coated Silver Nanoparticles as Insulin Fibril Inhibitors

Bera

Ghosh

Goswami

et al. 2023

ACS Appl. Nano Mater.

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To explore the impact of polymer-coated silver nanoparticles (PC-AgNPs) on the extent of the insulin aggregation process, herein, we have synthesized three copolymers comprising poly(ethylene glycol) methyl ether methacrylate (PEGMA) and tert-butoxycarbonyl (Boc)-protected amino acid (alanine, leucine, and phenylalanine) containing methacrylate monomers, via reversible addition-fragmentation chain transfer (RAFT) polymerization. After deprotection of the Boc groups, the as-prepared water-soluble copolymers were coated on silver nanoparticles (Ag NPs), and the role of these NPs on insulin aggregation pathways was examined by multifarious spectroscopic and microscopic techniques. The extent of the inhibitory effect against the insulin fibrillation process was found to be related to the surface properties of the NPs, with the highest inhibitory effect detected for phenylalanine-based polymer-coated Ag NPs (PPhe-AgNPs). Using circular dichroism (CD) spectroscopy and Nile red (NR) fluorescence spectroscopy, we investigated the conformational changes and examined the role of hydrophobic interaction in inhibiting the aggregation properties of insulin upon treatment with PC-AgNPs. Furthermore, PC-AgNPs were also able to disintegrate the matured insulin fibrils and efficiently decreased the fibril-induced cytotoxicity, as confirmed by transmission electron microscopy (TEM) and the hemolysis study, respectively. Together, our findings established the novel amino acid-based PC-AgNPs as potent nanomaterials with 77–96% insulin fibril inhibition and marked disaggregation of matured insulin fibrils.

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