The Role of Oviductal Cells in Activating Stallion Spermatozoa

Leemans, Bart; Gadella, Bart M.; Stout, T.A.E.; Schauwer, Catharina De; Nelis, Hilde; Hoogewijs, Maarten; Soom, Ann Van

doi:10.1016/j.jevs.2016.06.003

Cited by 2 publications

(2 citation statements)

References 78 publications

(116 reference statements)

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“…Then the more spermatozoa responding to hypoosmotic test, more live spermatozoa with motility.High correlation with trypan blue intact acrosome (TBlia and TBdia), could be considered favorable because when is detected good motility and the stallions with this characteristic is selected to reproduction, they are indirectly selected by another characteristic that is important. The moment of semen evaluation the acrosome must have the acrosome intact, because acrosome reaction must occur only during the process of fertilization in uterine tubal (Leemans et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Pantaneiro stallions: did the environment influence seminal traits and testicular biometry?

Ribas,

Rua,

Silva

et al. 2024

Preprint

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The aim of this present study was to evaluate the testis biometry and seminal characteristicsof the Pantaneiro horse breed in two season of year. Th experiment was performed in Pantanal region of Brazil, with evaluation of seven Pantaneiro stallions aged from 4 to 12 years and raised in extensive system. Testis traits and semen of the stallions were collected during the rainy season (RS - April) and dry season (DS - October).Seminalvolume, sperm concentration, total number of spermatozoa (TNSTZ), motility, vigor, sperm major defects, sperm total defects, hypoosmotic swelling test, trypan blues test and pH were evaluated. The right and left testicular (RTL and LTL), the right and left testicular height (RTH and LTH) and the right and left testicular width (RTW and LTW) were measured and testicle volume (TV) was calculated. Statistical analysis was performed to verify the effect of season on testis biometry traits and semen traits.Correlations of all characteristics were calculated.There was effect of season on TNSTZ and sperm major defects. All others war not different between RS and DS. The testicular volume means was 179.4 ± 8.6 cm3. There was no effect of season on testicular biometry traits.High and medium correlations were observed among testis length (TL), testis width (TW), TV with TNSTZ. Motility had high correlation with hypoosmotic test. High magnitude correlation was also observed between hypoosmotic test and intact membrane on trypan blue test. It suggests that the integrity of the membrane is essential for a good function of the kinetic of the spermatozoa.The results showed that the Pantaneiro stallions is adapted to the specific tropical environment and not presenting reproductive seasonality, being able to have your semen collected during the entire year without affect its quality also without testicular variation. Furthermore,the testicular length, width and testis volume showed highly representative as a criterion to selection of Pantaneiro stallions to reproduction.

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Section: Discussionmentioning

confidence: 99%

Pantaneiro stallions: did the environment influence seminal traits and testicular biometry?

Ribas,

Rua,

Silva

et al. 2024

Preprint

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“…Our decision to evaluate inseminations over a relatively long interval of 6 h was based on studies that had demonstrated ejaculates build semen reservoirs on the epithelial cells of the isthmus tubae uterinae (6,16,31,35). It can be hypothesized that with longer intervals, fresh loads of spermatozoa would arrive in an oviduct already containing sperm cell reservoirs and conditions would allow selected sperm cells to participate in fertilization.…”

Section: Discussionmentioning

confidence: 99%

Increase of pregnancy rate after multiple periovulatory inseminations in mares

Huber

Amsler

Vidondo

et al. 2019

Tierarztl Prax Ausg G

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Objective There exist differences in the reproductive behavior of stallions and mares under free-running and domestic in-hand breeding conditions. Contrary to artificial insemination programs, a stallion mates a mare multiple times per estrus under natural conditions. The objective of this study was to determine if multiple periovulatory artificial inseminations (MI), four times in two different time intervals instead of two, would result in increased pregnancy outcome or higher incidences of breeding induced endometritis. Material and methods Eighty-two estrous mares were allocated randomly to one of three experimental groups. They were administered intramuscular deslorelin (1.25 mg) to induce ovulation, and 24 hours later, they were inseminated either twice (group DI), four times in relatively short intervals (group MISI) or four times in relatively long intervals (MILI), after division of one commercial insemination dose (either frozen-thawed or chilled-warmed semen) into two or four portions, respectively. Uterine sampling for bacteriological and cytological analysis was conducted directly before the first insemination and 24 hours after the last insemination. Results Mares of the MI groups showed a higher pregnancy rate with frozen-thawed semen than mares of the DI group. There was no difference in bacteriological and cytological results between the three groups. In addition, mares of the MISI group showed less intrauterine fluid accumulation 24 hours after the last insemination than mares of the DI and MILI groups. Conclusion and clinical relevance We suggest that multiple periovulatory inseminations with frozen-thawed semen in a close time frame to ovulation lead to an increase of pregnancy results per cycle, and that they do not lead to impaired inflammatory reactions of the uterus in healthy fertile mares.

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The Role of Oviductal Cells in Activating Stallion Spermatozoa

Cited by 2 publications

References 78 publications

Pantaneiro stallions: did the environment influence seminal traits and testicular biometry?

Pantaneiro stallions: did the environment influence seminal traits and testicular biometry?

Increase of pregnancy rate after multiple periovulatory inseminations in mares

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