The role of ovarian steroids in reproductive plasticity in Hoplias malabaricus (Teleostei: Characiformes: Erythrinidae) in tropical reservoirs with different degrees of pollution

Gomes, Ana Carolina; Tolussi, Carlos Eduardo; Ribeiro, Carla; Honji, Renato Massaaki; Moreira, Renata Guimarães

doi:10.1016/j.ygcen.2014.10.008

Cited by 24 publications

(16 citation statements)

References 58 publications

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“…The higher percentages of n-3 PUFA, including EPA and DHA, in stomach contents and tissues of both species from the hypereutrophic reservoir (Bil) suggest that food resources in this reservoir were not as deficient in n-3 LC-PUFA as initially expected. Because of the occurrence of cyanobacterial blooms in the Bil reservoir [35,36] we expected to find higher percentages of 18:2n-6 and 18:3n-3 in the food resources, since these are the main FA produced by cyanobacteria [7]. Instead, besides high percentages of 18:3n-3, we also found high percentages of n-3 LC-PUFA, mainly EPA in the stomach contents of fish captured from the hypereutrophic reservoir, which contradicts the expected pattern for this reservoir [24].…”

Section: Discussionmentioning

confidence: 99%

“…Sampling was carried out in the Taquacetuba branch (23°48′16.5″S; 46°38′33.26″W; Fig. 1d), which is considered hypereutrophic due to high urbanization and occasional cyanobacterial blooms [35,36,39]. Moreover, high concentrations of heavy metals have been found in this reservoir, such as cadmium, lead, copper, mercury, and nickel [35].…”

Section: Study Sitementioning

confidence: 99%

“…1a, b; Table 1). Further limnological details about these reservoirs are available in Gomes et al [35]. The mesotrophic Ponte Nova Reservoir (PN) is located in a watershed area under environmental protection, far from urban centers and industries.…”

Section: Study Sitementioning

confidence: 99%

“…The mesotrophic Ponte Nova Reservoir (PN) is located in a watershed area under environmental protection, far from urban centers and industries. This region is occupied by Atlantic Forest and preserved riparian vegetation and lowland along the waterway [35,38]. Sampling was carried out in Rio Claro branch (PN; 23°34′36.5″S; 45°54′23.9″W; Fig.…”

Section: Study Sitementioning

confidence: 99%

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Fatty Acid Composition of Tropical Fish Depends on Reservoir Trophic Status and Fish Feeding Habit

Gomes

Tolussi

Boëchat

et al. 2016

Lipids

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Eutrophication results in a deficiency of n-3 LC-PUFA (long-chain polyunsaturated fatty acids) in aquatic food chains, affecting fish nutrition and physiology. The trophic transfer of FA (fatty acids) to fish species of different feeding habits was investigated in two reservoirs in southeast Brazil-the mesotrophic Ponte Nova Reservoir (PN) and the hypereutrophic Billings Reservoir (Bil). Total FA profile of stomach contents and adipose tissue, triacylglycerols (TAG), and phospholipids (PL) from liver and muscle of the omnivorous Astyanax fasciatus and the carnivorous Hoplias malabaricus were analyzed by gas chromatography. A prevalence of n-6PUFA, as 18:2n-6 (linoleic acid) and 20:4n-6 (arachidonic acid, ARA) was observed in the stomach contents and in the tissues of A. fasciatus from the PN reservoir. In contrast, n-3 LC-PUFA, as 20:5n-3 (eicosapentaenoic acid, EPA) was accumulated in fish tissues from Bil, resulting in higher n3/n6 and EPA/ARA ratios, compared to fish from PN. This differential FA accumulation was also observed for H. malabaricus, but differences were slightly minor, and no changes were observed in the EPA/ARA ratios between fish from both reservoirs. Regardless reservoir, FA profiles of TAG resembled that of their diet, whereas FA profiles of PL were more conservative and mainly comprised by LC-PUFA. We conclude that reservoir trophic status affected the FA composition of food resources available to these fish species, resulting in differential allocation of n-3 and n-6 FA. As expected, FA profile of the investigated fish species also reflected their feeding habit and physiological demands.

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Section: Discussionmentioning

confidence: 99%

Section: Study Sitementioning

confidence: 99%

Section: Study Sitementioning

confidence: 99%

Section: Study Sitementioning

confidence: 99%

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Fatty Acid Composition of Tropical Fish Depends on Reservoir Trophic Status and Fish Feeding Habit

Gomes

Tolussi

Boëchat

et al. 2016

Lipids

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“…Os níveis plasmáticos de 17β-estradiol (E 2 ) e 20β-dihydroxy-4-pregnen-3-one (17α, 20β-DHP) nas fêmeas, e de testosterona (T) e 11-ketotestosterona (11KT) nos machos, foram quantificados pela técnica de ELISA com o emprego de kits comerciais da Cayman (Cayman Chemical Company, Michigan, USA). Estes kits foram previamente validados em duas espécies de Characiformes, Hoplias malabaricus (GOMES et al, 2015) e Salminus hilarii (MOREIRA et al, 2015). Todas as amostras de plasma foram analisadas em duplicatas e seguindo as instruções específicas de cada kit.…”

Section: Dosagens De Esteroides Sexuaisunclassified

Gonadotropinas e seus receptores em <i>Astyanax altiparanae </i>(Teleostei, Characiformes): caracterização molecular e expressão espaço-temporal durante o ciclo reprodutivo em cativeiro.

Jesus¹

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Agradeço primeiramente a Deus, por guiar meus caminhos e conceder forças para eu vencer as incertezas e desafios da carreira acadêmica. E ainda, por me proporcionar experiências incríveis e conhecer pessoas admiráveis durante toda essa jornada na USP de São Paulo e na Holanda. À minha família, especialmente aos meus pais, Célia Aparecida e Waesso Orcélio de Oliveira, bem como minha avó materna, Evangelha Oliveira, por serem o alicerce da minha formação pessoal, moral e apoiar incondicionalmente minhas aspirações profissionais. Aos meus amigos de longa data, Paulo Roberto, Bárbara Rauber, Ísis Braga, Valéria Ferreira, Thiago Batista e Estael Lima, pela amizade incondicional. Aos amigos, Dra. Lorenna Rezende (UFMT), Dr. Fernando Ladd (UFRN) e Dra. Alliny Ladd (FMVZ-USP). Vocês contribuíram muito para o início da minha carreira acadêmica na USP. Sempre serei grato por todos os conselhos e ajuda nos momentos mais difíceis. À minha orientadora, Dra. Maria Inês Borella, pelos conhecimentos compartilhados, confiança depositada e pelas muitas oportunidades a mim dadas, para a realização do mestrado, doutorado e do Doutorado Sanduíche na Holanda. Ainda, agradeço por apoiar e incentivar minhas escolhas profissionais. A todos componentes do Laboratório de Endocrinologia de Peixes, atuais (Gisele Melo, Marília Paiva, Mônica Cassel e Telma Monezi) e que já nos deixaram (Giovana Branco, Chayrra Gomes, Mateus Adolfi, Sara Gomes e Fabiano Costa), pelos diversos momentos científicos e pessoais compartilhados. E também à Rejane Funes, minha primeira aluna de Iniciação Cientifica, pelo aprendizado e experiências. Ao Cruz Alberto Mendonza Rigonati, técnico do nosso laboratório, pelo apoio em todas as etapas deste trabalho, durante as inúmeras viagens à CESP de Paraibuna, pelos cortes histológicos primorosos e, ainda, pela amizade e confiança ao longo desses anos. A todos docentes do Departamento de Biologia Celular e do Desenvolvimento do Instituto de Ciências Biomédicas da USP, pelos ensinamentos e ambiente científico profícuo proporcionado. À Coordenação do Programa de Pós-Graduação em Biologia Celular e Tecidual, pela busca incessante de aperfeiçoamento e fortalecimento deste programa e pela atenção dada aos seus pós-graduandos. À Secretária do Programa de Pós-Graduação em Biologia Celular e Tecidual, Regina Valbom, por todos os serviços prestados, sempre com muita presteza e paciência. À Profa. Dra. Irene Yan (ICB-USP) e seu ex-aluno, Dr. Felipe Vieceli, pela colaboração desenvolvida nas etapas iniciais deste trabalho e ainda por guiar meus primeiros passos no "Universo" da Biologia Molecular. Aos colegas do Laboratório de Embriologia Molecular dos Vertebrados (ICB-USP), Tatiane Kanno, Maraysa Melo e Carolina Purcell, pela convivência, descontração e ajuda durante as inúmeras reações de cDNA, RT-PCR e clonagem molecular realizadas nesse trabalho. À Doutoranda e colega Renata Gomes pelo auxílio na técnica de Western Blott. E a Profa. Dra. Alison Colquhoun (ICB-USP) por ceder seu laboratório e reagentes para o desenvolvimento dest...

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Molecular characterization of different preproGnRHs in Astyanax altiparanae (Characiformes): Effects of GnRH on female reproduction

Chehade

Amaral

Branco

et al. 2020

Molecular Reproduction Devel

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Gonadotropin‐releasing hormone (GnRH) is a key molecule in the initiation of the hypothalamic–pituitary–gonadal axis. Thus, knowledge about GnRH may contribute to the effectiveness of species reproduction. Using a Neotropical tetra Astyanax altiparanae as a fish model species, the GnRH forms were characterized at the molecular level and the role of injected GnRHs in vivo was evaluated. The full‐length complementary DNA (cDNA) sequences of preproGnRH2 (612 bp) and preproGnRH3 (407 bp) of A. altiparanae were obtained, and the GnRH1 form was not detected. The cDNA sequences of preproGnRH2 and preproGnRH3 were found to be conserved, but a change in the amino acid at position 8 of the GnRH3 decapeptide of A. altiparanae was observed. All the injected GnRHs stimulated lhβ messenger RNA (mRNA) expression but not fshβ mRNA expression, and only GnRH2 was able to increase maturation‐inducing steroid (MIS) levels and possibly stimulate oocyte release. Furthermore, only GnRH2 was able to start the entire reproductive hormonal cascade and induce spawning.

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The role of ovarian steroids in reproductive plasticity in Hoplias malabaricus (Teleostei: Characiformes: Erythrinidae) in tropical reservoirs with different degrees of pollution

Cited by 24 publications

References 58 publications

Fatty Acid Composition of Tropical Fish Depends on Reservoir Trophic Status and Fish Feeding Habit

Fatty Acid Composition of Tropical Fish Depends on Reservoir Trophic Status and Fish Feeding Habit

Gonadotropinas e seus receptores em <i>Astyanax altiparanae </i>(Teleostei, Characiformes): caracterização molecular e expressão espaço-temporal durante o ciclo reprodutivo em cativeiro.

Molecular characterization of different preproGnRHs in Astyanax altiparanae (Characiformes): Effects of GnRH on female reproduction

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