The Role of Oestradiol in the Uterine Peristalsis in the Perfused Swine Uterus

Abstract

This study was designed to examine the effects of oestradiol (E2) on sperm transport in the swine uterus. The bicornuate swine uterus is optimal for the study of the uterine transport and peristalsis because the influence of various factors can be examined on each uterine horn independently. Forty swine uteri (with or without ovarectomy) were perfused for a period of up to 7 h. Two different E2 concentrations (3 or 30 pg/ml) in the perfusion medium were administered for 30 min unilaterally. Through an intracervical catheter 1 ml of a high concentrated dextran blue solution was administered directly in the upper part of the cervix. After bilateral perfusion of the swine uterus with a bolus of 0.3 IU oxytocin the distribution of coloured particles was assessed macroscopically before and after incision of the uterine horns. Coloration was evaluated by two observers blinded to the site-specific administration of E2. In the 10 ovarectomized uteri with the 3 pg/ml E2 concentration a unilateral distribution towards the side of oestradiol administration was observed in six uteri, in four it was a bilateral distribution. In the 10 non-ovarectomized uteri with the 3 pg/ml E2 concentration a uni- and ipsilateral coloration was observed in five uteri, in five it was a bilateral distribution. In the 20 uteri with 30 pg/ml E2, a unilateral coloration of the uterus horns was observed in all uteri. Oestradiol is one of the main factors, which influences the direction of the sperm transport in a dose-dependent manner, in the perfused swine uterus.

Uterus Cryopreservation: Maintenance of Uterine Contractility by the Use of Different Cryoprotocols

“…2004; Revel et al. 2004), extracorporal perfusion of swine uteri (Maltaris et al. 2005b, 2006; Mueller et al.…”

Genistein Alters the Release of Oxytocin, Prostaglandins, Cortisol and LH during Insemination in Gilts

“…1982). Oestrogens act synergistic with oxytocin to support uterine contractility in perfused swine uteri (Maltaris et al. 2006), and oxytocin itself causes uterine contractions and acts indirectly by stimulating prostaglandin release in bovine epithelial cells (Asselin et al.…”

The intrauterine to intra-arterial pressure ratio: a new parameter for the study of uterine contractility physiology

“…This perfusion model is able to keep the uterus in a functional condition and is suitable for the study of physiological questions (Bulletti et al, 1993(Bulletti et al, , 2001. The development of an extracorporeal perfusion model of the swine uterus enabled the research group to carry out general assessments of uterine contractility and peristalsis (Dittrich et al, 2003(Dittrich et al, , 2006(Dittrich et al, , 2008aMaltaris et al, 2005Maltaris et al, , 2006aMueller et al, 2006a,b).…”