The Role of Nuclear Factor Kappa B in Lens Epithelial Cell Proliferation Using a Capsular Bag Model

Lee, Seok Jong; Bae, Sangwoo; Seomun, Young; Son, Min Jung; Joo, Choun‐Ki

doi:10.1159/000128162

Cited by 11 publications

(10 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This process was named pyroptosis [41,42]. It has been reported that NF-κB inhibits the proliferation and modulates the progression of lens epithelial cells [49]. In hippocampal neurons, treatment with TLR4 antibody, bpV(pic), or PDTC decreased the LPS-induced nuclear translocation of NF-κB and resulted in the down-regulation of TNF-α and IL-1β expression [50], which revealed that the TLR4-mediated NF-κB signaling pathway is associated with the activation of a neuroinflammatory response.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of NF-κB by Pyrrolidine Dithiocarbamate Prevents the Inflammatory Response in a Ligature-Induced Peri-Implantitis Model: A Canine Study

Jiang²,

Wang

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: The roles of toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-κB) in peri-implantitis are unclear. Here, we used a canine model of peri-implantitis to explore the effects of inhibiting NF-κB with pyrrolidine dithiocarbamate (PDTC) on the inflammatory response in ligature-induced peri-implantitis. Methods: After successfully establishing the peri-implantitis model, beagles were randomly assigned to normal, model or PDTC groups. ELISA tests were used to determine the levels of interleukin (IL)-1, IL-6, IL-8 and tumor necrosis factor alpha (TNF-α). Immunohistochemistry was employed to assess the expression of NF-κB p65. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to determine the mRNA levels of TLR4 and NF-κB p65, and western blot analysis was used to measure the protein levels of TLR4 in periodontal tissues from each group. Periodontal ligament fibroblasts (PDLFs) were cultured and subsequently classified into PDLF normal, PDLF model, PDLF LPS, PDLF PDTC, and PDLF LPS + PDTC groups. An immunofluorescence assay was used to measure the expression level of NF-κB p65. The CCK-8 assay and flow cytometry were performed to evaluate cell proliferation and apoptosis. Results: The in vitro results indicated that NF-κB p65 and TLR4 were upregulated in canine periodontal tissues, and PDTC could suppress the expression levels of NF-κB p65 and TLR4. Inflammation could increase TLR4 protein expression in canine periodontal tissue, and PDTC could inhibit the inflammation-induced increase in TLR4 protein expression. These results revealed that PDTC could reverse the LPS-induced increases in the levels of IL-1, IL-6, IL-8 and TNF-α. In vivo, the results demonstrated that PDTC inhibited the LPS-induced NF-κB p65 upregulation, and PDTC could reverse the inhibitory effect of the PDLF model + LPS on the proliferation of periodontal fibroblasts. The results also showed that in the PDLF model, LPS promoted PDLF apoptosis by inducing implant periodontitis in canines, but PDTC inhibited the PDLF apoptosis and relieved implant periodontitis in canines. Conclusion: Based on our results, we concluded that PDTC can inhibit the expression of NF-κB and alleviate the inflammatory response induced by LPS, thereby preventing periodontal inflammation and reducing the development of peri-implantitis.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibition of NF-κB by Pyrrolidine Dithiocarbamate Prevents the Inflammatory Response in a Ligature-Induced Peri-Implantitis Model: A Canine Study

Jiang²,

Wang

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Further, in a recent study redox-sensitive transcription factor NF-κB has been implicated in LEC proliferation and PCO 25. We have shown that aldose reductase (AR), a polyol pathway enzyme that reduces glucose and various lipid peroxidation-derived aldehydes and their glutathione conjugates, mediates ROS signals initiated by growth factors, cytokines, high glucose and bacterial endotoxin (Lypopolysaccheride; LPS) via activation of NF-κB 26-29.…”

Section: Introductionmentioning

confidence: 99%

Prevention of Posterior Capsular Opacification through Aldose Reductase Inhibition

Yadav¹,

Ighani-Hosseinabad

Kuijk

et al. 2009

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

Purpose The purpose of this study was to evaluate the effect of aldose reductase (AR) inhibition on Posterior capsular opacification (PCO) using pig eye capsular bag model. Methods Pig eye capsular bags were prepared by capsulorhexis and cultured in medium without or with AR inhibitors for 7 days. Immunostaining was performed in paraformaldehyde-fixed capsular bags to determine the expression of proliferating cell nuclear antigen (PCNA), α-smooth muscle actin (SMA), β-crystallin, and intercellular adhesion molecule (ICAM)-1. We examined the effect of AR inhibition on basic fibroblast growth factor (b-FGF) -induced mitogenic signaling in cultured human lens epithelial cells (HLEC). Cell growth was assessed by MTT assay and cell counting, the expression of α-SMA, β-crystallin and ICAM-1 by Western Blot and immunocytochemical analysis, protein kinases by Western blot analysis, and NF-κB activation by Gel shift and reporter assays. Results During culture of pig eye capsular bags, residual cells on both the anterior and posterior capsule showed vigorous growth. Treatment with AR inhibitors significantly prevented the lens epithelial cell growth in capsular bags and expression of α-SMA, β-crystallin and ICAM-1. HLEC showed a dose-dependent response to b-FGF, proliferation at lower (<20 ng/ml) and differentiation/transdifferentiation at higher (>50 ng/ml) concentrations. Inhibition of AR also prevented the b-FGF -induced activation of ERK1/2, JNK and NF-κB in HLEC. Conclusions Our results suggest that AR is required for lens epithelial cell growth and differentiation/transdifferentiation in the capsular bags indicating that inhibition of AR could be a potential therapeutic target in the prevention of PCO.

show abstract

“…76 The sealed-capsule irrigation system is a device that has been developed to selectively deliver an agent to target lens epithelial cells following cataract removal. 77 The agents/mechanisms currently investigated to prevent PCO include nuclear factor kappaB (NF-kappaB), 78 proteasome inhibition, 79 macrophage depletion 80 as well as drugs like cyclosporin A 81 and mitomycin-C. 82 Moving away from the surgical options, drugs have been tried as a therapeutic option for cataracts. N-acetyl carnosine is one of the topical agents that have been developed for this purpose.…”

Section: Future Developmentsmentioning

confidence: 99%

Advances in cataract surgery

Ashwin

Shah

Wolffsohn

2009

Clinical and Experimental Optometry

View full text Add to dashboard Cite

Cataract surgery is a technique described since recorded history, yet it has greatly evolved only in the latter half of the past century. The development of the intraocular lens and phacoemulsification as a technique for cataract removal could be considered as the two most significant strides that have been made in this surgical field. This review takes a comprehensive look at all aspects of cataract surgery, starting from patient selection through the process of consent, anaesthesia, biometry, lens power calculation, refractive targeting, phacoemulsification, choice of intraocular lens and management of complications, such as posterior capsular opacification, as well as future developments. As the most common ophthalmic surgery and with the expanding range of intraocular lens options, optometrists have an important and growing role in managing patients with cataract.

show abstract

The Role of Nuclear Factor Kappa B in Lens Epithelial Cell Proliferation Using a Capsular Bag Model

Cited by 11 publications

References 35 publications

Inhibition of NF-κB by Pyrrolidine Dithiocarbamate Prevents the Inflammatory Response in a Ligature-Induced Peri-Implantitis Model: A Canine Study

Inhibition of NF-κB by Pyrrolidine Dithiocarbamate Prevents the Inflammatory Response in a Ligature-Induced Peri-Implantitis Model: A Canine Study

Prevention of Posterior Capsular Opacification through Aldose Reductase Inhibition

Advances in cataract surgery

Contact Info

Product

Resources

About