Pro-environmental behavior (PEB) has always been a hot topic in academic and practical, and it is highly necessary to explore the research progress and development trend of PEB. Based on 1038 relevant literatures published and the Web of Science core database, this paper used citation analysis, co-word analysis and cluster analysis methods to systematically analyze the dynamic evolution process of PEB’s research topics, knowledge base and subject areas. The results show that PEB research is currently in a period of rapid growth. And PEB research presents typical multidisciplinary characteristics, mainly focuses on Psychology-Education-Social, Economics-Economic-Political, Environmental-Toxicology-Nutrition and other disciplines. Then, this study also finds that the PEB research hotspots mainly concentrated in seven directions such as “Environmental cognition, emotion and motivation process”, etc., which can be further classified into three horizontal levels and three vertical levels. This study will provide valuable theoretical and practical reference for the future research of PEB.