“…The cells were grown as monolayer cultures in RPMI (Roswell Park Memorial Institute) 1640 medium (JRH Biosciences, Kansas, USA), supplemented with 10% fetal calf serum (Gibco, Paisley, UK), 2 mM L-glutamine (Sigma, St Louis, MO, USA), 200 units l 21 insulin (SIGMA), and 1% penicillin/streptomycin (Gibco) at 37°C in air containing 5% CO 2 (Edin et al 2012(Edin et al , 2013. The cells were grown as monolayer cultures in RPMI (Roswell Park Memorial Institute) 1640 medium (JRH Biosciences, Kansas, USA), supplemented with 10% fetal calf serum (Gibco, Paisley, UK), 2 mM L-glutamine (Sigma, St Louis, MO, USA), 200 units l 21 insulin (SIGMA), and 1% penicillin/streptomycin (Gibco) at 37°C in air containing 5% CO 2 (Edin et al 2012(Edin et al , 2013.…”