The Role of Nitric Oxide in Stem Cell Biology

Caballano-Infantes, Estefanía; Cahuana, Gladys M.; Bedoya, Francisco J.; Salguero-Aranda, Carmen; Tejedo, Juan R.

doi:10.3390/antiox11030497

Cited by 12 publications

(12 citation statements)

References 135 publications

(187 reference statements)

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“…NO is involved in the regulation of cell growth, survival, apoptosis, proliferation, and differentiation [56,64]. In healthy odontoblasts, eNOS [43,65], and in inflamed odontoblasts, iNOS and ONOO − [7] have been described.…”

Section: Discussionmentioning

confidence: 99%

Inflammation of the Human Dental Pulp Induces Phosphorylation of eNOS at Thr495 in Blood Vessels

et al. 2022

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The activity of endothelial nitric oxide synthase (eNOS) in endothelial cells increased with the phosphorylation of the enzyme at Ser1177 and decreased at Thr495. The regulation of the phosphorylation sites of eNOS at Ser1177 and Thr495 in blood vessels of the healthy and inflamed human dental pulp is unknown. To investigate this, healthy and carious human third molars were immersion-fixed and decalcified. The localization of eNOS, Ser1177, and Thr495 in healthy and inflamed blood vessels was examined in consecutive cryo-sections using quantitative immunohistochemical methods. We found that the staining intensity of Ser1177 in healthy blood vessels decreased in inflamed blood vessels, whereas the weak staining intensity of Thr495 in healthy blood vessels strongly increased in inflamed blood vessels. In blood vessels of the healthy pulp, eNOS is active with phosphorylation of the enzyme at Ser1177. The phosphorylation of eNOS at Thr495 in inflamed blood vessels leads to a decrease in eNOS activity, contributing to eNOS uncoupling and giving evidence for a decrease in NO and an increase in O2− production. Since the formation of the tertiary dentin matrix depends on intact pulp circulation, eNOS uncoupling and phosphorylation of eNOS at Thr495 in the inflamed pulp blood vessels should be considered during caries therapy.

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Section: Discussionmentioning

confidence: 99%

Inflammation of the Human Dental Pulp Induces Phosphorylation of eNOS at Thr495 in Blood Vessels

et al. 2022

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“…In plants, NO has been shown to play role in developmental and physiological processes, including floral transition, reaction to hypoxia, as well as immune and defense responses 4,21–23 . Additionally, several lines of evidence have suggested that redox state plays an important role in stem cell homeostasis, including the key redox component RNS and reactive oxygen species (ROS) 2,6,24–26 . NO and ROS are involved in controlling differentiation of embryonic stem cells in animals 2,27,28 and more recently, they have been shown to act as important signals in root and shoot stem cell systems of plants 3,6,7,25,29 .…”

Section: Mainmentioning

confidence: 99%

Nitric Oxide controls shoot meristem activity via regulation of DNA methylation

Zeng,

Zhao,

Liang

et al. 2023

Preprint

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Despite the importance of Nitric Oxide (NO) as signaling molecule in both plant and animal development1–7, the regulatory mechanisms downstream of NO remain largely unclear. Here, we show that NO is involved inArabidopsisshoot stem cell control via modifying expression and activity ofARGONAUTE 4(AGO4), a core component of the RNA directed DNA Methylation (RdDM) pathway. Mutations in components of the RdDM pathway cause meristematic defects, and reduce responses of the stem cell system to NO signaling. Importantly, we found that the stem cell inducing WUSCHEL transcription factor directly interacts with AGO4 in an NO dependent manner, explaining how these two signaling systems may converge to modify DNA methylation patterns. Taken together, our results reveal that NO signaling plays an important role in controlling plant stem cell homeostasis via the regulation ofde novoDNA methylation.

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“…Second, the production of NO is known to play a role in the maintenance of pluripotency (reviewed in [ 100 , 101 ]). Indeed, low μM of NO have been shown to protect mESCs from death or differentiation following leukemia inhibitory factor (LIF) withdrawal, through a blocked caspase 3 activation, an upregulation of anti-apoptotic genes such as Bcl2 , and a downregulation of differentiation genes such as Brachyury or Gata4 (GATA Binding Protein 4) [ 102 ].…”

Section: Succinate As a Paracrine Effectormentioning

confidence: 99%

Succinate as a New Actor in Pluripotency and Early Development?

Detraux

Renard

2022

Metabolites

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Pluripotent cells have been stabilized from pre- and post-implantation blastocysts, representing respectively naïve and primed stages of embryonic stem cells (ESCs) with distinct epigenetic, metabolic and transcriptomic features. Beside these two well characterized pluripotent stages, several intermediate states have been reported, as well as a small subpopulation of cells that have reacquired features of the 2C-embryo (2C-like cells) in naïve mouse ESC culture. Altogether, these represent a continuum of distinct pluripotency stages, characterized by metabolic transitions, for which we propose a new role for a long-known metabolite: succinate. Mostly seen as the metabolite of the TCA, succinate is also at the crossroad of several mitochondrial biochemical pathways. Its role also extends far beyond the mitochondrion, as it can be secreted, modify proteins by lysine succinylation and inhibit the activity of alpha-ketoglutarate-dependent dioxygenases, such as prolyl hydroxylase (PHDs) or histone and DNA demethylases. When released in the extracellular compartment, succinate can trigger several key transduction pathways after binding to SUCNR1, a G-Protein Coupled Receptor. In this review, we highlight the different intra- and extracellular roles that succinate might play in the fields of early pluripotency and embryo development.

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The Role of Nitric Oxide in Stem Cell Biology

Cited by 12 publications

References 135 publications

Inflammation of the Human Dental Pulp Induces Phosphorylation of eNOS at Thr495 in Blood Vessels

Inflammation of the Human Dental Pulp Induces Phosphorylation of eNOS at Thr495 in Blood Vessels

Nitric Oxide controls shoot meristem activity via regulation of DNA methylation

Succinate as a New Actor in Pluripotency and Early Development?

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