The role of neutrophil chemotaxis activity as an immunologic biomarker to predict mortality in critically-ill patients with severe sepsis

Srisawat, Nattachai; Kulvichit, Win; Tungsanga, Somkanya; Peerapornratana, Sadudee; Vorasitchai, Suttinan; Tangkanakul, Chakorn; Lumlertgul, Nuttha; Komaenthammasophon, Chalermchai; Praditpornsilpa, Kearkiat; Tungsanga, Kriang; Eiam‐Ong, Somchai

doi:10.1016/j.jcrc.2020.01.020

Cited by 10 publications

(10 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have compared EAA and BG level of our COVID-19 patients with the data from our previous study in 136 severe sepsis or septic shock patients (Additional file 2: Figure S7a and S7b) [23,24]. This demonstrated that COVID-19 patients had EAA and BG levels comparable to severe sepsis patients.…”

Section: Clinical Characteristics At Baseline and Outcomesmentioning

confidence: 78%

Endotoxemia and circulating bacteriome in severe COVID-19 patients

Sirivongrangson

Kulvichit

Payungporn

et al. 2020

ICMx

Self Cite

View full text Add to dashboard Cite

Background When severe, COVID-19 shares many clinical features with bacterial sepsis. Yet, secondary bacterial infection is uncommon. However, as epithelium is injured and barrier function is lost, bacterial products entering the circulation might contribute to the pathophysiology of COVID-19. Methods We studied 19 adults, severely ill patients with COVID-19 infection, who were admitted to King Chulalongkorn Memorial Hospital, Bangkok, Thailand, between 13th March and 17th April 2020. Blood samples on days 1, 3, and 7 of enrollment were analyzed for endotoxin activity assay (EAA), (1 → 3)-β-d-glucan (BG), and 16S rRNA gene sequencing to determine the circulating bacteriome. Results Of the 19 patients, 13 were in intensive care and 10 patients received mechanical ventilation. We found 8 patients with high EAA (≥ 0.6) and about half of the patients had high serum BG levels which tended to be higher in later in the illness. Although only 1 patient had a positive blood culture, 18 of 19 patients were positive for 16S rRNA gene amplification. Proteobacteria was the most abundant phylum. The diversity of bacterial genera was decreased overtime. Conclusions Bacterial DNA and toxins were discovered in virtually all severely ill COVID-19 pneumonia patients. This raises a previously unrecognized concern for significant contribution of bacterial products in the pathogenesis of this disease.

show abstract

Section: Clinical Characteristics At Baseline and Outcomesmentioning

confidence: 78%

Endotoxemia and circulating bacteriome in severe COVID-19 patients

Sirivongrangson

Kulvichit

Payungporn

et al. 2020

ICMx

Self Cite

View full text Add to dashboard Cite

show abstract

“…In other studies of PLHIV and non-HIV-infected cohorts, it is becoming increasingly apparent that markers of inflammation are more predictive of mortality than specific disease-related factors. [18–20]…”

Section: Discussionmentioning

confidence: 99%

Predictors of in-hospital mortality in a cohort of people living with HIV (PLHIV) admitted to an academic medical intensive care unit from 2009 to 2014: A retrospective cohort study

et al. 2022

View full text Add to dashboard Cite

Outcomes for critically ill people living with human immunodeficiency virus (PLHIV) have changed with the use of antiretroviral therapy (ART). To identify these outcomes and correlates of mortality in a contemporary critically ill cohort in an urban academic medical center in Baltimore, a city with a high burden of HIV, we conducted a retrospective cohort study of individuals admitted to a medical intensive care unit (MICU) at a tertiary care center between 2009 and 2014. PLHIV who were at least 18 years of age with an index MICU admission of ≥24 hours during the 5-year study period were included in this analysis. Data were obtained for participants from the time of MICU admission until hospital discharge and up to 180 days after MICU admission. Logistic regression was used to identify independent predictors of hospital mortality. Between June 2009 and June 2014, 318 PLHIV admitted to the MICU met inclusion criteria. Eighty-six percent of the patients were non-Hispanic Blacks. Poorly controlled HIV was very common with 70.2% of patients having a CD4 cell count <200 cells/mm3 within 3 months prior to admission and only 34% of patients having an undetectable HIV viral load. Hospital mortality for the cohort was 17%. In a univariate model, mortality did not differ by demographic variables, CD4 cell count, HIV viral load, or ART use. Regression analysis adjusted by relevant covariates revealed that MICU patients admitted from the hospital ward were 6.4 times more likely to die in hospital than those admitted from emergency department. Other positive predictors were a diagnosis of end-stage liver disease, cardiac arrest, ventilator-dependent respiratory failure, vasopressor requirement, non-Hodgkin lymphoma, and symptomatic cytomegalovirus disease. In conclusion, in this critically ill cohort with HIV infection, most predictors of mortality were not directly related to HIV and were similar to those for the general population.

show abstract

“…The chemiluminescence intensity reflecting the bactericidal capacity of neutrophils showed a significant decrease in septic patients, which was correlated with the severity and poor prognosis of sepsis ( 24 , 82 , 83 ). Neutrophil chemotaxis activity, measured by transmigration assay, was found to independently correlate with 28-day mortality in critical illness with sepsis ( 84 ). A recently published study proposed an analyzing platform for the dynamic assessment of neutrophil chemotaxis, in which they revealed an obviously altered chemotactic function of neutrophils in patients complicated with severe infection by applying quantitative indicators ( 85 ).…”

Section: Monitoring the Alterations Of The Innate Immune Systemmentioning

confidence: 99%

Advances in Immune Monitoring Approaches for Sepsis-Induced Immunosuppression

Yao

Ren²,

Zheng³

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

Sepsis represents a life-threatening organ dysfunction due to an aberrant host response. Of note is that majority of patients have experienced a severe immune depression during and after sepsis, which is significantly correlated with the occurrence of nosocomial infection and higher risk of in-hospital death. Nevertheless, the clinical sign of sepsis-induced immune paralysis remains highly indetectable and ambiguous. Given that, specific yet robust biomarkers for monitoring the immune functional status of septic patients are of prominent significance in clinical practice. In turn, the stratification of a subgroup of septic patients with an immunosuppressive state will greatly contribute to the implementation of personalized adjuvant immunotherapy. In this review, we comprehensively summarize the mechanism of sepsis-associated immunosuppression at the cellular level and highlight the recent advances in immune monitoring approaches targeting the functional status of both innate and adaptive immune responses.

show abstract

The role of neutrophil chemotaxis activity as an immunologic biomarker to predict mortality in critically-ill patients with severe sepsis

Cited by 10 publications

References 46 publications

Endotoxemia and circulating bacteriome in severe COVID-19 patients

Endotoxemia and circulating bacteriome in severe COVID-19 patients

Predictors of in-hospital mortality in a cohort of people living with HIV (PLHIV) admitted to an academic medical intensive care unit from 2009 to 2014: A retrospective cohort study

Advances in Immune Monitoring Approaches for Sepsis-Induced Immunosuppression

Contact Info

Product

Resources

About