The Role of Neutr Ophils and Their Apoptosis in There Solution of Inflammation

Pavlyshyn, Halyna; Sarapuk, Iryna

doi:10.5604/2081-2021.1192740

Cited by 2 publications

(2 citation statements)

References 60 publications

(100 reference statements)

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“…Активні форми кисню пошкоджують мітохондріальні мембрани, спричиняють вихід біологічно активних речовин у цитозоль (цитохром С, апоптозіндукуючий фактор), які запускають каскад каспазних реакцій та призводять до апоптозу. Цікавим є те, що мітохондрії, як основні мішені вільних радикалів, одночасно виступають джерелом продукції активних форм кисню в клітині, які, в свою чергу, також можуть активувати апоптоз, що тим самим створює так зване «замкнуте коло» [17].…”

Section: результати та їх обговоренняunclassified

Viability and Types of Death of Blood Leukocytes in Rats With Polycystic Ovary Syndrome Under Conditions of Intermittent Cold Exposure

Zhulikova,

Myroshnychenko,

Nakonechna

et al. 2023

Fiziol Zh

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The aim of our work is to assess the viability and determine the types of death of blood leukocytes in rats with polycystic ovary syndrome (PCOS) under conditions of intermittent cold exposure. The study was performed on 40 immature female rats of the WAG population, aged 27 days, divided into 5 groups of 8 animals each. Group 1 included intact rats. In group 2, rats were subcutaneously injected with 0.2 ml of purified and sterilized olive oil daily for 25 days. In group 3, rats were exposed to periodic cold exposure daily for 25 days. In group 4, dehydroepiandrosterone-induced PCOS was modeled in rats. In group 5, rats were injected with dehydroepiandrosterone against the background of periodic cold exposure. Leukocyte viability and death pathways were determined using a FACS Canto II flow cytometer (Becton Dickinson, USA). Rats with dehydroepiandrosteroneinduced PCOS showed a decrease in the percentage of viable leukocytes and an increase in the percentage of leukocytes in the early stage of apoptosis, while the number of leukocytes in the late stage of apoptosis and necrotic leukocytes did not change. In rats, on the background of the introduction of dehydroepiandrosterone under conditions of intermittent cold exposure, the percentage of viable leukocytes, as well as leukocytes at different stages of apoptosis and necrosis, corresponded to the physiological norm. Thus, the authors revealed a decrease in the viability of neutrophilic leukocytes in the blood of rats with dehydroepiandrosterone-induced PCOS due to the activation of apoptosis processes, as evidenced by an increase in the percentage of leukocytes at the initial stages of apoptosis. Intermittent cold exposure (4 h daily at +4°C for 25 days) against the background of dehydroepiandrosterone administration to rats had an anti-apoptotic effect, normalizing the viability of neutrophilic blood leukocytes.

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Section: результати та їх обговоренняunclassified

Viability and Types of Death of Blood Leukocytes in Rats With Polycystic Ovary Syndrome Under Conditions of Intermittent Cold Exposure

Zhulikova,

Myroshnychenko,

Nakonechna

et al. 2023

Fiziol Zh

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“…Our research data on the increase of apoptosis processes in the trophoblast of the chorionic villi of the placenta in IDA, which are identical to the results of other researchers [4,14], can be related to the development of hypoxia in response to anemia which quickly depletes cell energy systems and leads to its death [14]. The intensification of Bax-dependent apoptosis processes in acute inflammation of the chorionic and basal plates of the placenta probably occurs with the contribution of cytotoxic leukocytes, which are able to use a special transmembrane respiratory chain of the plasma membrane to form superoxides and bombard the target cell stimulating apoptosis, in particular through oxidation in particular due to the oxidation of phosphatidylserine in the plasma membrane of the target cell [15]. In chronic inflammation, the intensification of apoptotic processes is mainly due to the increasing severity of inflammation and increased activity of free radical processes, which is confirmed by our previously obtained data on high levels of nitroperoxides in placental structures [16], which begin to induce apoptosis.…”

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confidence: 99%

Immunohistochemical Study of Trophoblasts Cellular Regulation Processes in Chorioamnionitis and Basal Deciduitis Combined with Iron-Deficiency Anemia in Gravidas

Ilika¹,

Garvasiuk²,

Іlika³

2021

Ukr. ž. med. bìol. sportu

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The purpose of the study is to establish quantitative parameters of cell proliferation and apoptosis in the trophoblast of the chorionic villi in chorioamnionitis and basal deciduitis combined with iron-deficiency anemia in gravidas by means of immunohistochemical method. Materials and methods. 198 placentas were examined. The immunohistochemical procedure was performed using primary antibodies against Ki-67 and Bax antigen with imaging by a polymer system with diaminobenzidine dye. The number of Ki-67-positive nuclei of the chorionic villi trophoblast was counted, and for the Bax antigen, the optical density of the immunohistochemical staining was measured by means of microdensitometric method. Comparison of differences in mean trends was performed using the odd Student’s two-sided t-test (p≤0.05). Results and discussion. The number of Ki-67-positive trophoblast nuclei in acute chorioamnionitis with iron-deficiency anemia in gravidas was 56±3.8 ‰, and the relative units of optical density of immunohistochemical staining for protein Bax – 0.234±0.0012, in chronic – 59±3.6 ‰ and 0.2, respectively. The number of Ki-67-positive nuclei of the chorionic villi trophoblast was counted. Placentas with acute as well as chronic chorioamnionitis and basal deciduitis showed even higher averages (p <0.001). In acute basal deciduitis in anemia, the number of Ki-67-positive trophoblast nuclei was 56±3.2 ‰, the average optical density of immunohistochemical staining for protein Bax – 0.236±0.0016, in chronic – 57±3.7 and 0.249±0.0015, respectively. It should be noted that in chronic chorioamnionitis and basal deciduitis, these rates were higher than in acute. With the same regularity the average indicators of optical density of immunohistochemical staining on protein Bax in a trophoblast of chorionic villi at comorbid iron-deficiency anemia concerning an inflammation without anemia increase. We have shown that proliferative activity in iron-deficiency anemia varies with gestational age and placental prematurity, but iron-deficiency anemia in gravidas and chorionic tree maturation both individually and in combination lead to the intensification of these processes. We obtained a justification for the arithmetic mean thickness and volume of the placenta relative to observations of placenta with inflammation without anemia in this comorbid pathology. Conclusion. Iron-deficiency anemia in gravidas leads to the intensification of proliferative processes and Bax-dependent apoptosis in the trophoblast of the chorionic villi of the placenta relative to the placenta from physiological pregnancy. In acute as well as in chronic chorioamnionitis and basal deciduitis, the proliferative activity and apoptotic processes in the trophoblast of the chorionic villi of the placenta increase, while comorbid iron-deficiency anemia in gravidas intensifies only the processes of Bax-dependent apoptosis

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The Role of Neutr Ophils and Their Apoptosis in There Solution of Inflammation

Cited by 2 publications

References 60 publications

Viability and Types of Death of Blood Leukocytes in Rats With Polycystic Ovary Syndrome Under Conditions of Intermittent Cold Exposure

Viability and Types of Death of Blood Leukocytes in Rats With Polycystic Ovary Syndrome Under Conditions of Intermittent Cold Exposure

Immunohistochemical Study of Trophoblasts Cellular Regulation Processes in Chorioamnionitis and Basal Deciduitis Combined with Iron-Deficiency Anemia in Gravidas

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