The role of nanocomposites in bone regeneration

Rogel, Micah R.; Qiu, Hengwei; Ameer, Guillermo A.

doi:10.1039/b804692a

Cited by 84 publications

(58 citation statements)

References 70 publications

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“…In comparison with conventional composites, they seem to induce cell response more efficiently and generally possess enhanced mechanical performances [4,12].…”

Section: Introductionmentioning

confidence: 99%

Magnetic poly(ε-caprolactone)/iron-doped hydroxyapatite nanocomposite substrates for advanced bone tissue engineering

Gloria

Russo

D’Amora

et al. 2013

J. R. Soc. Interface.

177

126

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In biomedicine, magnetic nanoparticles provide some attractive possibilities because they possess peculiar physical properties that permit their use in a wide range of applications. The concept of magnetic guidance basically spans from drug delivery and hyperthermia treatment of tumours, to tissue engineering, such as magneto-mechanical stimulation/activation of cell constructs and mechanosensitive ion channels, magnetic cell-seeding procedures, and controlled cell proliferation and differentiation. Accordingly, the aim of this study was to develop fully biodegradable and magnetic nanocomposite substrates for bone tissue engineering by embedding irondoped hydroxyapatite (FeHA) nanoparticles in a poly(1-caprolactone) (PCL) matrix. X-ray diffraction analyses enabled the demonstration that the phase composition and crystallinity of the magnetic FeHA were not affected by the process used to develop the nanocomposite substrates. The mechanical characterization performed through small punch tests has evidenced that inclusion of 10 per cent by weight of FeHA would represent an effective reinforcement. The inclusion of nanoparticles also improves the hydrophilicity of the substrates as evidenced by the lower values of water contact angle in comparison with those of neat PCL. The results from magnetic measurements confirmed the superparamagnetic character of the nanocomposite substrates, indicated by a very low coercive field, a saturation magnetization strictly proportional to the FeHA content and a strong history dependence in temperature sweeps. Regarding the biological performances, confocal laser scanning microscopy and AlamarBlue assay have provided qualitative and quantitative information on human mesenchymal stem cell adhesion and viability/proliferation, respectively, whereas the obtained ALP/DNA values have shown the ability of the nanocomposite substrates to support osteogenic differentiation.

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“…In comparison with conventional composites, they seem to induce cell response more efficiently and generally possess enhanced mechanical performances [4,12].…”

Section: Introductionmentioning

confidence: 99%

Magnetic poly(ε-caprolactone)/iron-doped hydroxyapatite nanocomposite substrates for advanced bone tissue engineering

Gloria

Russo

D’Amora

et al. 2013

J. R. Soc. Interface.

177

126

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“…El hueso es un tejido compuesto, con una estructura jerárquica multiescalar (Rogel et al, 2008;Turner-Walker, 2008;Weiner, 2010) (Fig. 1).…”

Section: La Histología Normal Del Hueso Y Sus Alteraciones (Bioerosión)unclassified

“…un fosfato de calcio cristalino análogo a la hidroxiapatita), que se encuentra estrechamente vinculada, en el nivel estructural más profundo -i.e. en el nivel sub-nanoestructural (Rogel et al, 2008)-, con una fase orgánica conformada con una matriz de proteína, predominantemente colágeno de Tipo I (Turner-Walker, 2008;Tütken y Vennemann, 2011). Las moléculas en forma de triple hélice de colágeno, junto con los cristales de bioapatita que se disponen entre las mismas, se asocian entre sí en forma paralela y escalonada constituyendo, sucesivamente, fibrillas y fibras que componen el soporte estructural de los huesos (nivel nanoestructural; Rogel et al, 2008).…”

Section: La Histología Normal Del Hueso Y Sus Alteraciones (Bioerosión)unclassified

“…En el siguiente nivel (i.e. sub-microestructural; Rogel et al, 2008), las fibras se disponen en capas cilíndricas superpuestas denominadas lamelas, en el centro de las cuales existe un canal, denominado de Havers que, en el hueso vivo, da paso a pequeños vasos sanguíneos y fibras nerviosas. En la zona de unión de las lamelas concéntricas se encuentran micro-cavidades denominadas lagunas, cada una de ellas habitada por una célula ósea u osteocito.…”

Section: La Histología Normal Del Hueso Y Sus Alteraciones (Bioerosión)unclassified

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Evaluación De La Diagénesis Ósea Mediante El Uso De Microscopía Electrónica De Barrido (Meb): Aproximaciones Analíticas Aplicables a Muestras Arqueológicas

Barrientos¹,

Sarmiento²,

Galligani³

2016

rev arg antrop biol

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RESUMEN El objetivo del presente trabajo es realizar una revisión de las aproximaciones analíticas aplicables al estudio de la diagénesis ósea -dentro del campo más amplio de la tafonomía-que se basan en el uso de la microscopía electrónica de barrido (MEB). Para ello, se presenta a) una síntesis de los conocimientos actuales acerca de los procesos diagenéticos que afectan a la integridad histológica de los restos óseos, tanto humanos como faunísticos, en depósitos sedimentarios terrestres; b) una exposición abreviada de los principios generales de la microscopía electrónica, particularmente de la MEB y c) un comparación de las diferencias -en cuanto a requerimientos, información obtenible y ventaPalabRaS clavE hueso; alteración histológica; imágenes jas/desventajas asociadas-entre los análisis centrados en los dos principales tipos de imágenes producidas por un MEB, es decir, las de electrones retrodispersados y las de electrones secundarios. Se concluye que, dada la disponibilidad relativamente amplia de esta tecnología, el factor crítico para una incorporación más activa del MEB en la investigación arqueológica y bioantropológica no es el acceso al equipamiento, sino la apropiada identificación de los problemas de investigación a resolver, así como la adecuada selección y ejecución de las técnicas de observación y análisis a emplear. Rev Arg Antrop Biol 18(2), 2016. doi:10.17139/ raab.2016.0018.02.03 KEywoRdS bone; histological alteration; images abSTRacT The aim of this paper is to review analytic approaches to bone diagenesis based on the use of scanning electron microscopy (SEM). In order to do this, we present a) a synthesis of current knowledge about diagenetic processes affecting the histological integrity of human and faunal skeletal remains in terrestrial sedimentary deposits; b) a brief description of the general principles of electron microscopy, particularly of SEM, and c) a comparison of the main differences -in terms of requirements, recoverable information, and associated pros and cons-between analyses centered on the use of either of the two main kinds of images produced by a SEM, i.e., backscattered electron images and secondary electron images. It is concluded that, given the relatively wide availability of this technology, the critical factor to a more active involvement of SEM in archaeological and bioanthropological research is not the access to equipment, but the correct identification of the research problems to be solved, as well as the proper selection and implementation of the observational and analytical techniques to be employed.

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“…A variety of nanocomposites have been fabricated from polymers and HAp [78,79]. In particular, bioactive inorganic materials may promote a strong bond with the scaffold surface by precipitation of an intermediate layer of HAp, so mineralizing the polymer matrix [75].…”

Section: Composite Hydrogels With Mineral Phases For Hard Tissue Regementioning

confidence: 99%

Hydrogel-Based Platforms for the Regeneration of Osteochondral Tissue and Intervertebral Disc

et al. 2012

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Hydrogels currently represent a powerful solution to promote the regeneration of soft and hard tissues. Primarily, they assure efficient bio-molecular interactions with cells, also regulating their basic functions, guiding the spatially and temporally complex multi-cellular processes of tissue formation, and ultimately facilitating the restoration of structure and function of damaged or dysfunctional tissues. In order to overcome basic drawbacks of traditional synthesized hydrogels, many recent strategies have been implemented to realize multi-component hydrogels based on natural and/or synthetic materials with tailored chemistries and different degradation kinetics. Here, a critical review of main strategies has been proposed based on the use of hydrogels-based devices for the regeneration of complex tissues, i.e., osteo-chondral tissues and intervertebral disc.

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The role of nanocomposites in bone regeneration

Cited by 84 publications

References 70 publications

Magnetic poly(ε-caprolactone)/iron-doped hydroxyapatite nanocomposite substrates for advanced bone tissue engineering

Magnetic poly(ε-caprolactone)/iron-doped hydroxyapatite nanocomposite substrates for advanced bone tissue engineering

Evaluación De La Diagénesis Ósea Mediante El Uso De Microscopía Electrónica De Barrido (Meb): Aproximaciones Analíticas Aplicables a Muestras Arqueológicas

Hydrogel-Based Platforms for the Regeneration of Osteochondral Tissue and Intervertebral Disc

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