The role of NADPH oxidase and MAP kinase phosphatase in UV‐B‐dependent gene expression in <i>Arabidopsis</i>

Kalbina, Irina; Strid, Åke

doi:10.1111/j.1365-3040.2006.01555.x

Cited by 55 publications

(37 citation statements)

References 45 publications

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“…In addition to providing protection from photodamage, we show here that Phe metabolism could counteract the increase in ROS concentration as part of the stress response to high-frequency radiation ( Fig. 2; A-H-Mackerness et al, 2001; Kalbina and Strid, 2006;Consentino et al, 2015).…”

Section: Suc Supply Restores Guard Cell Number While Asn and Nh 4 Ac mentioning

confidence: 57%

The dehydratase ADT3 affects ROS homeostasis and cotyledon development

et al. 2016

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During the transition from seed to seedling, emerging embryos strategically balance available resources between building up defenses against environmental threats and initiating the developmental program that promotes the switch to autotrophy. We present evidence of a critical role for the phenylalanine (Phe) biosynthetic activity of AROGENATE DEHYDRATASE3 (ADT3) in coordinating reactive oxygen species (ROS) homeostasis and cotyledon development in etiolated Arabidopsis (Arabidopsis thaliana) seedlings. We show that ADT3 is expressed in the cotyledon and shoot apical meristem, mainly in the cytosol, and that the epidermis of adt3 cotyledons contains higher levels of ROS. Genome-wide proteomics of the adt3 mutant revealed a general down-regulation of plastidic proteins and ROS-scavenging enzymes, corroborating the hypothesis that the ADT3 supply of Phe is required to control ROS concentration and distribution to protect cellular components. In addition, loss of ADT3 disrupts cotyledon epidermal patterning by affecting the number and expansion of pavement cells and stomata cell fate specification; we also observed severe alterations in mesophyll cells, which lack oil bodies and normal plastids. Interestingly, up-regulation of the pathway leading to cuticle production is accompanied by an abnormal cuticle structure and/or deposition in the adt3 mutant. Such impairment results in an increase in cell permeability and provides a link to understand the cell defects in the adt3 cotyledon epidermis. We suggest an additional role of Phe in supplying nutrients to the young seedling.

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Section: Suc Supply Restores Guard Cell Number While Asn and Nh 4 Ac mentioning

confidence: 57%

The dehydratase ADT3 affects ROS homeostasis and cotyledon development

et al. 2016

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“…With regard to the enzymatic source of H 2 O 2 mediating UV-B-induced stomatal closure, our previous study has provided pharmacological evidence that 0.8 W m 22 UV-B-induced H 2 O 2 generation in broad bean guard cells depends on peroxidases but not NADPH oxidases (He et al, 2011a). However, UV-B is reported to stimulate mRNA transcript levels and the activity of NADPH oxidases (Rao et al, 1996;A-H-Mackerness et al, 2001;Casati and Walbot, 2003), and mutants in the Arabidopsis AtrbohD and AtrbohF genes show reduced H 2 O 2 accumulation in response to UV-B (Kalbina and Strid, 2006), suggesting that NADPH oxidases are also potential sources of H 2 O 2 induced by UV-B. To explore whether different doses of UV-B can activate distinct sources of H 2 O 2 to induce stomatal closure, we examined H 2 O 2 sources during stomatal closure induced by 0.5 and 0.7 W m 22 UV-B.…”

Section: Ga Plays An Important Role In Uv-b Guard Cell Signalingmentioning

confidence: 96%

Role and Interrelationship of Gα Protein, Hydrogen Peroxide, and Nitric Oxide in Ultraviolet B-Induced Stomatal Closure in Arabidopsis Leaves

Zhang

et al. 2013

Plant Physiology

125

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Heterotrimeric G proteins have been shown to transmit ultraviolet B (UV-B) signals in mammalian cells, but whether they also transmit UV-B signals in plant cells is not clear. In this paper, we report that 0.5 W m 22 UV-B induces stomatal closure in Arabidopsis (Arabidopsis thaliana) by eliciting a cascade of intracellular signaling events including Ga protein, hydrogen peroxide (H 2 O 2 ), and nitric oxide (NO). UV-B triggered a significant increase in H 2 O 2 or NO levels associated with stomatal closure in the wild type, but these effects were abolished in the single and double mutants of AtrbohD and AtrbohF or in the Nia1 mutants, respectively. Furthermore, we found that UV-B-mediated H 2 O 2 and NO generation are regulated by GPA1, the Ga-subunit of heterotrimeric G proteins. UV-B-dependent H 2 O 2 and NO accumulation were nullified in gpa1 knockout mutants but enhanced by overexpression of a constitutively active form of GPA1 (cGa). In addition, exogenously applied H 2 O 2 or NO rescued the defect in UV-B-mediated stomatal closure in gpa1 mutants, whereas cGa AtrbohD/AtrbohF and cGa nia1 constructs exhibited a similar response to AtrbohD/ AtrbohF and Nia1, respectively. Finally, we demonstrated that Ga activation of NO production depends on H 2 O 2 . The mutants of AtrbohD and AtrbohF had impaired NO generation in response to UV-B, but UV-B-induced H 2 O 2 accumulation was not impaired in Nia1. Moreover, exogenously applied NO rescued the defect in UV-B-mediated stomatal closure in the mutants of AtrbohD and AtrbohF. These findings establish a signaling pathway leading to UV-B-induced stomatal closure that involves GPA1-dependent activation of H 2 O 2 production and subsequent Nia1-dependent NO accumulation.

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“…Arabidopsis mkp1 mutants are hypersensitive to methyl methanesulfonate (MMS) and UV-B stress (1,(21)(22)(23). On the other hand, MKP1 is a negative regulator of MPK6-mediated PAMP responses and resistance against bacteria (24,25).…”

mentioning

confidence: 99%

Phosphorylation and Stabilization of Arabidopsis MAP Kinase Phosphatase 1 in Response to UV-B Stress

Besteiro

Ulm

2013

Journal of Biological Chemistry

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Background: Arabidopsis MKP1 regulates MPK3 and MPK6 and has a crucial role in the UV-B stress response. Results: Plant treatment with UV-B results in a gel mobility shift and accumulation of MKP1. Conclusion: MKP1 is a phosphoprotein that is stabilized in response to UV-B. Significance: MKP1 phosphorylation and stabilization identifies novel post-translational regulation of a plant MAP kinase phosphatase in vivo.

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The role of NADPH oxidase and MAP kinase phosphatase in UV‐B‐dependent gene expression in Arabidopsis

Abstract: ABSTRACT

Cited by 55 publications

References 45 publications

The dehydratase ADT3 affects ROS homeostasis and cotyledon development

The dehydratase ADT3 affects ROS homeostasis and cotyledon development

Role and Interrelationship of Gα Protein, Hydrogen Peroxide, and Nitric Oxide in Ultraviolet B-Induced Stomatal Closure in Arabidopsis Leaves

Phosphorylation and Stabilization of Arabidopsis MAP Kinase Phosphatase 1 in Response to UV-B Stress

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