The Role of Mitochondria in Methamphetamine-induced inhibitory effects on osteogenesis of Mesenchymal Stem Cells

Shen, Yulai; Wu, Lu; Wang, Jun; Wu, Xin; Zhang, Xuemei

doi:10.1016/j.ejphar.2018.02.049

Cited by 14 publications

(8 citation statements)

References 66 publications

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“…Mitophagy is an important way to maintain mitochondrial network homeostasis and protect cells from the deleterious effects of mitotoxicity [ 29 ]. The appropriate roles of mitochondria are considered vital for successful differentiation of osteoblasts [ 30 , 31 ]. Undifferentiated cells have mitochondria with relatively low activity, and upon initiation of the differentiation process, there is a significant increase in mtDNA copy number, protein levels of respiratory enzymes, transcription of mitochondria-associated genes, oxygen consumption rate, and ATP content [ 30 , 32 ] in osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

PINK1 deficiency impairs osteoblast differentiation through aberrant mitochondrial homeostasis

Lee

Kim

et al. 2021

Stem Cell Res Ther

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Background PTEN-induced kinase 1 (PINK1) is a serine/threonine-protein kinase in mitochondria that is critical for mitochondrial quality control. PINK1 triggers mitophagy, a selective autophagy of mitochondria, and is involved in mitochondrial regeneration. Although increments of mitochondrial biogenesis and activity are known to be crucial during differentiation, data regarding the specific role of PINK1 in osteogenic maturation and bone remodeling are limited. Methods We adopted an ovariectomy model in female wildtype and Pink1−/− mice. Ovariectomized mice were analyzed using micro-CT, H&E staining, Masson’s trichrome staining. RT-PCR, western blot, immunofluorescence, alkaline phosphatase, and alizarin red staining were performed to assess the expression of PINK1 and osteogenic markers in silencing of PINK1 MC3T3-E1 cells. Clinical relevance of PINK1 expression levels was determined via qRT-PCR analysis in normal and osteoporosis patients. Results A significant decrease in bone mass and collagen deposition was observed in the femurs of Pink1−/− mice after ovariectomy. Ex vivo, differentiation of osteoblasts was inhibited upon Pink1 downregulation, accompanied by impaired mitochondrial homeostasis, increased mitochondrial reactive oxygen species production, and defects in mitochondrial calcium handling. Furthermore, PINK1 expression was reduced in bones from patients with osteoporosis, which supports the practical role of PINK1 in human bone disease. Conclusions In this study, we demonstrated that activation of PINK1 is a requisite in osteoblasts during differentiation, which is related to mitochondrial quality control and low reactive oxygen species production. Enhancing PINK1 activity might be a possible treatment target in bone diseases as it can promote a healthy pool of functional mitochondria in osteoblasts.

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Section: Discussionmentioning

confidence: 99%

PINK1 deficiency impairs osteoblast differentiation through aberrant mitochondrial homeostasis

Lee

Kim

et al. 2021

Stem Cell Res Ther

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“…In an in vitro study, methamphetamine exposure dampened the osteogenic differentiation of BMSCs due to abnormal OXPHOS and reduced ATP generation and mitochondrial membrane depolarization. These mitochondrial malfunctions were attributed to damaged mitochondrial biogenesis and mitochondrial fusion (Shen et al, 2018a). Similarly, treatment with carbon black Printex 90, a representative carbonaceous particle toxicant, induced mitochondrial dysfunction in BMSCs, which is closely related to suppressed mitochondrial biogenesis and mitochondrial dynamics, ultimately resulting in impaired osteogenic potential of BMSCs (Shen et al, 2018b).…”

Section: Mitochondrial Dynamics In Mscs Under Physical Stress and Toxinsmentioning

confidence: 99%

“…According to the literature, stress often results in fragmentation but not elongation events in mitochondria in MSCs, which is attributed to dramatic enhanced mitochondrial fission with/without inhibited mitochondrial fusion (He et al, 2018;Marycz et al, 2018;Shen et al, 2018a;Ma et al, 2019). In addition, the suppression effect on both mitochondrial fission and fusion was observed in BMSCs when they were treated with high levels of ferric ammonium citrate, a commonly used agent to induce iron overload (Yao et al, 2019).…”

Section: Multifaceted Effects Of Mitochondrial Dynamics Under Stressmentioning

confidence: 99%

Mitochondrial Dynamics: Fission and Fusion in Fate Determination of Mesenchymal Stem Cells

Ren

Chen

et al. 2020

Front. Cell Dev. Biol.

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Mesenchymal stem cells (MSCs) are pivotal to tissue homeostasis, repair, and regeneration due to their potential for self-renewal, multilineage differentiation, and immune modulation. Mitochondria are highly dynamic organelles that maintain their morphology via continuous fission and fusion, also known as mitochondrial dynamics. MSCs undergo specific mitochondrial dynamics during proliferation, migration, differentiation, apoptosis, or aging. Emerging evidence suggests that mitochondrial dynamics are key contributors to stem cell fate determination. The coordination of mitochondrial fission and fusion is crucial for cellular function and stress responses, while abnormal fission and/or fusion causes MSC dysfunction. This review focuses on the role of mitochondrial dynamics in MSC commitment under physiological and stress conditions. We highlight mechanistic insights into modulating mitochondrial dynamics and mitochondrial strategies for stem cell-based regenerative medicine. These findings shed light on the contribution of mitochondrial dynamics to MSC fate and MSC-based tissue repair.

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“…The following reports discuss how mitochondrial numbers and mitochondrial activity have been determined in different cells and tissues. [40][41][42][43] Furthermore, mitochondria in stem cells and induced pluripotent stem cells are poorly developed and low in number; mitochondrial function and structure have even been suggested as indicators of stem cell competence. 44 The hypothesis of the present review is that the effects of PBM on different tissues can be explained by taking into account two main factors.…”

Section: Mitochondria and Cellsmentioning

confidence: 99%

Review of light parameters and photobiomodulation efficacy: dive into complexity

2018

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Photobiomodulation (PBM) therapy, previously known as low-level laser therapy, was discovered more than 50 years ago, yet there is still no agreement on the parameters and protocols for its clinical application. Some groups have recommended the use of a power density less than 100 mW∕cm 2 and an energy density of 4 to 10 J∕cm 2 at the level of the target tissue. Others recommend as much as 50 J∕cm 2 at the tissue surface. The wide range of parameters that can be applied (wavelength, energy, fluence, power, irradiance, pulse mode, treatment duration, and repetition) in some cases has led to contradictory results. In our review, we attempt to evaluate the range of effective and ineffective parameters in PBM. Studies in vitro with cultured cells or in vivo with different tissues were divided into those with higher numbers of mitochondria (muscle, brain, heart, nerve) or lower numbers of mitochondria (skin, tendon, cartilage). Graphs were plotted of energy density against power density. Although the results showed a high degree of variability, cells/tissues with high numbers of mitochondria tended to respond to lower doses of light than those with lower number of mitochondria. Ineffective studies in cells with high mitochondrial activity appeared to be more often due to over-dosing than to under-dosing.

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The Role of Mitochondria in Methamphetamine-induced inhibitory effects on osteogenesis of Mesenchymal Stem Cells

Cited by 14 publications

References 66 publications

PINK1 deficiency impairs osteoblast differentiation through aberrant mitochondrial homeostasis

PINK1 deficiency impairs osteoblast differentiation through aberrant mitochondrial homeostasis

Mitochondrial Dynamics: Fission and Fusion in Fate Determination of Mesenchymal Stem Cells

Review of light parameters and photobiomodulation efficacy: dive into complexity

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