The role of miRNAs in the diagnosis of stable atherosclerosis of different arterial territories: A critical review

Teixeira, António; Ferreira, V; Pereira-da-Silva, Tiago; Ferreira, Rui Cruz

doi:10.3389/fcvm.2022.1040971

Cited by 4 publications

(2 citation statements)

References 130 publications

(170 reference statements)

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“…Some miRNAs displayed opposite expression trends, like miR-126, which was upregulated in renal artery stenosis and downregulated in carotid and lower limb plaques [ 66 ]. Conversely, miR-221-3p/miR-30 downregulation and miR-21 upregulation were found in both carotid and lower limbs arteries, whereas miR-145 was downregulated in carotid and coronary arteries [ 67 ].…”

Section: Micrornas (Mirnas)mentioning

confidence: 99%

Heterogeneity and Differentiation of the Human Arterial Tree: Focus on microRNA Expression in Vascular Disease

Ciavarella,

Motta,

Capri

et al. 2024

Biomolecules

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Human arteries show structural and functional peculiarities according to the nutrient and oxygen needs of a specific vascular district. This architectural heterogeneity is reflected in the pathological setting of cardiovascular diseases (CVDs). Indeed, the responsiveness to cardiovascular risk factors, and the morphological and molecular patterns are discriminating factors among CVDs affecting different vascular beds. MicroRNAs (miRNAs) are endogenous regulators of gene expression and fine-tuners of vascular cell differentiation; thus, these non-coding RNAs can modulate arterial heterogeneity. The identification of an artery-specific miRNA signature would be promising in the therapy of CVDs, especially in patients who are frail and elderly. In the present review, we will provide a concise description of the arterial tree heterogeneity on a structural and cellular basis, mainly in the pathological context. Secondly, we will address the miRNA potential as crucial mediators of arterial heterogeneity, focusing on the abdominal aorta and femoral artery, with the final goal of strengthening the search for more targeted therapies in CVDs and stratification approaches in patients who are frail and elderly.

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Section: Micrornas (Mirnas)mentioning

confidence: 99%

Heterogeneity and Differentiation of the Human Arterial Tree: Focus on microRNA Expression in Vascular Disease

Ciavarella,

Motta,

Capri

et al. 2024

Biomolecules

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“…Since one miRNA can target and regulate hundreds of genes (mRNAs), miRNAs have also gained considerable attention as biomarkers or putative indicators of mechanistic pathways in disease states. Furthermore, differential plasma miRNA profiles have been described for many diseases, including fatty liver [89] and atherosclerosis [90,91]. Dysregulation or altered miRNA expression/function has been implicated in diabetes [92] and cardiovascular disease [93,94].…”

Section: Exosome Mirnasmentioning

confidence: 99%

Multi-Omics Analysis of Circulating Exosomes in Adherent Long-Term Treated OSA Patients

Khalyfa,

Marin,

Sanz-Rubio

et al. 2023

IJMS

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Obstructive sleep apnea (OSA) is a highly prevalent chronic disease affecting nearly a billion people globally and increasing the risk of multi-organ morbidity and overall mortality. However, the mechanisms underlying such adverse outcomes remain incompletely delineated. Extracellular vesicles (exosomes) are secreted by most cells, are involved in both proximal and long-distance intercellular communication, and contribute toward homeostasis under physiological conditions. A multi-omics integrative assessment of plasma-derived exosomes from adult OSA patients prior to and after 1-year adherent CPAP treatment is lacking. We conducted multi-omic integrative assessments of plasma-derived exosomes from adult OSA patients prior to and following 1-year adherent CPAP treatment to identify potential specific disease candidates. Fasting morning plasma exosomes isolated from 12 adult patients with polysomnographically-diagnosed OSA were analyzed before and after 12 months of adherent CPAP therapy (mean ≥ 6 h/night) (OSAT). Exosomes were characterized by flow cytometry, transmission electron microscopy, and nanoparticle tracking analysis. Endothelial cell barrier integrity, wound healing, and tube formation were also performed. Multi-omics analysis for exosome cargos was integrated. Exosomes derived from OSAT improved endothelial permeability and dysfunction as well as significant improvement in tube formation compared with OSA. Multi-omic approaches for OSA circulating exosomes included lipidomic, proteomic, and small RNA (miRNAs) assessments. We found 30 differentially expressed proteins (DEPs), 72 lipids (DELs), and 13 miRNAs (DEMs). We found that the cholesterol metabolism (has04979) pathway is associated with lipid classes in OSA patients. Among the 12 subjects of OSA and OSAT, seven subjects had complete comprehensive exosome cargo information including lipids, proteins, and miRNAs. Multi-omic approaches identify potential signature biomarkers in plasma exosomes that are responsive to adherent OSA treatment. These differentially expressed molecules may also play a mechanistic role in OSA-induced morbidities and their reversibility. Our data suggest that a multi-omic integrative approach might be useful in understanding how exosomes function, their origin, and their potential clinical relevance, all of which merit future exploration in the context of relevant phenotypic variance. Developing an integrated molecular classification should lead to improved diagnostic classification, risk stratification, and patient management of OSA by assigning molecular disease-specific therapies.

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Association between aortic calcification and cytokine levels in patients with peripheral artery disease

Rantasalo,

Laukka,

Nikulainen

et al. 2024

Clinical Translational Sci

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Aortic calcification—a marker of advanced atherosclerosis in large arteries—associates with cardiovascular mortality and morbidity. Little is known about the soluble inflamJarmatory profiles involved in large artery atherosclerosis. We investigated the correlation between aortic calcification in the abdominal aorta and cytokine levels in a cohort of peripheral artery disease patients. Aortic calcification index was measured from computed tomography exams and circulating cytokine levels were analyzed from blood serum samples of 156 consecutive patients prior to invasive treatment of peripheral artery disease. The study included 156 patients (mean age 70.7 years, 64 (41.0%) women). The mean ankle‐brachial index (ABI) was 0.64 and the mean aortic calcification index (ACI) was 52.3. ACI was associated with cytokines cutaneous T‐cell‐attracting chemokine CTACK (β 23.08, SE 5.22, p < 0.001) and monokine induced by gamma‐interferon MIG (β 9.40, SE 2.82, p 0.001) in univariate linear regression. After adjustment with cardiovascular risk factors, CTACK and MIG were independently associated with ACI, β 17.9 (SE 5.22, p < 0.001) for CTACK and β 6.80 (SE 3.33, p 0.043) for MIG. CTACK was significantly higher in the patients representing the highest ACI tertile (highest vs. middle, 7.53 vs. 7.34 Tukeys HSD p‐value 0.023 and highest vs. lowest tertile 7.53 vs. 7.29, Tukeys HSD p‐value 0.002). MIG was significantly higher in the highest tertile versus lowest (7.65 vs. 7.30, Tukeys HSD p‐value 0.027). Cytokines CTACK and MIG are associated with higher ACI, suggesting that CTACK and MIG reflect atherosclerotic disease burden of the aorta. This might further suggest the possible association with other cardiovascular morbidities.

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The role of miRNAs in the diagnosis of stable atherosclerosis of different arterial territories: A critical review

Cited by 4 publications

References 130 publications

Heterogeneity and Differentiation of the Human Arterial Tree: Focus on microRNA Expression in Vascular Disease

Heterogeneity and Differentiation of the Human Arterial Tree: Focus on microRNA Expression in Vascular Disease

Multi-Omics Analysis of Circulating Exosomes in Adherent Long-Term Treated OSA Patients

Association between aortic calcification and cytokine levels in patients with peripheral artery disease

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