The role of MiRNA in polycystic ovary syndrome (PCOS)

Chen, Baiqi; Xu, Peng; Wang, Jing; Zhang, Chunping

doi:10.1016/j.gene.2019.04.082

Cited by 111 publications

(76 citation statements)

References 65 publications

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“…Increasing evidence indicates that abnormal expression of miRNAs has been detected in follicular fluid, granulosa cells, adipose tissue, serum, cumulus cells, and ovarian tissue of women with PCOS and plays vital role in the origin and progression of PCOS [12][13][14] . Moreover, acupuncture treatment could be used in the therapy of diseases such as stoke by restoring abnormal expression of miRNAs 36,37 .…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Acupuncture on MiRNA Expression Profiles in the Ovaries of Rats with PCOS and Insulin Resistance

Zhang

Huang

et al. 2020

Preprint

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Background: Polycystic ovary syndrome (PCOS) is one of the most commonendocrine and metabolic diseases of women of reproductive age. The majorcharacteristics of PCOS are anovulation, hyperandrogenism, infertility, and insulinresistance. Previous studies showed that acupuncture could promote menstruation,decrease testosterone levels, increase insulin sensitivity, and improve frequency ofovulation in women with PCOS. However, the mechanism of how acupunctureregulates the reproductive and metabolic abnormalities of PCOS remains unclear. Thepresent study investigated the differential expression of miRNAs in the ovaries of ratswith PCOS and insulin resistance (IR) after electroacupuncture (EA) treatment.Methods: A rat model of PCOS with IR was established by letrozole and a high-fatdiet. Rats in the PCOS+EA group received acupuncture treatment five times a week forfour weeks. The differentially expressed miRNAs of three rat ovaries of each group(n=3/group) were identified by deep sequencing. The differentially expressed miRNAsare shown by a hierarchical clustering heatmap and volcano plot. Gene ontology (GO)analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysiswere conducted to explore the potential target genes of the differentially expressedmiRNAs and identify their putative biological function. Eight of the differentiallyexpressed miRNAs were selected for validation by real-time quantitative PCR (qRTPCR).Results: The rats in the PCOS+EA group had significantly decreased body weight andincreased insulin sensitivity compared with the rats in the PCOS group. EA tend toreduce testosterone levels in the rats with PCOS and insulin resistance. The rats in thePCOS+EA group displayed fewer large cystic follicles, fewer atretic antral follicles,and more corpora lutea than the rats in the PCOS group. A total of 23 differentiallyexpressed miRNAs were identified in the PCOS with IR group after EA treatment,including 15 miRNAs that were upregulated and 8 miRNAs that were downregulated.GO and KEGG pathway analyses indicated that the predicted target genes were relatedto cellular processes, and metabolic pathways. Furthermore, qRT-PCR confirmed thatmiRNA-181 was significantly downregulated in the rat ovaries of the PCOS+EA groupcompared with the PCOS group.Conclusion: Our results indicate that differentially expressed miRNAs may mediate the effect of acupuncture on insulin sensitivity in PCOS with IR. It will be beneficial to study the mechanism of acupuncture in improving insulin resistance in women with PCOS.

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Section: Discussionmentioning

confidence: 99%

“…MiRNAs are involved in many physiological processes including cell growth, differentiation, proliferation, and metabolism 12 . Furthermore, several studies have shown that dysfunctional miRNAs are associated with the pathological mechanism of PCOS [13][14][15][16][17] . For example, Professor…”

Section: Introductionmentioning

confidence: 99%

The Effect of Acupuncture on MiRNA Expression Profiles in the Ovaries of Rats with PCOS and Insulin Resistance

Zhang

Huang

et al. 2020

Preprint

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“…These studies, however, are at variance with a recent study by Yin et al (2014) showing that miRNA-320 inhibits estradiol synthesis in and proliferation of mouse ovarian granulosa cells by targeting E2FA and SF-1 transcription factors. There are several additional reports showing PCOS-induced alterations in miRNAs expression as summarized in Supplementary Table 3 and in recent reviews (Sørensen et al 2014, Chen et al 2019.…”

Section: Journal Of Molecular Endocrinologymentioning

confidence: 92%

The role of miRNAs in regulating adrenal and gonadal steroidogenesis

Azhar

Dong

Shen

et al. 2020

Journal of Molecular Endocrinology

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miRNAs are endogenous noncoding single-stranded small RNAs of ~22 nucleotides in length that post-transcriptionally repress the expression of their various target genes. They contribute to the regulation of a variety of physiologic processes including embryonic development, differentiation and proliferation, apoptosis, metabolism, hemostasis and inflammation. In addition, aberrant miRNA expression is implicated in the pathogenesis of numerous diseases including cancer, hepatitis, cardiovascular diseases and metabolic diseases. Steroid hormones regulate virtually every aspect of metabolism, and acute and chronic steroid hormone biosynthesis is primarily regulated by tissue-specific trophic hormones involving transcriptional and translational events. In addition, it is becoming increasingly clear that steroidogenic pathways are also subject to post-transcriptional and post-translational regulations including processes such as phosphorylation/dephosphorylation, protein‒protein interactions and regulation by specific miRNAs, although the latter is in its infancy state. Here, we summarize the recent advances in miRNA-mediated regulation of steroidogenesis with emphasis on adrenal and gonadal steroidogenesis.

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“…In the literature, many clinical studies assessed circulating miRNA levels in peripheral blood for diagnosing, predicting, and monitoring human diseases (158)(159)(160)(161)(162)(163), including cardiac and vascular disease (CVD) (164)(165)(166)(167)(168)(169). For example, the combination of miR-34a-5p and fibrinogen levels have been reported as a useful tool in differentiating pre-thrombotic status in patients with stable coronary artery disease (165).…”

Section: Emerging Challenge Of Bench Studies For Early Detecting and mentioning

confidence: 99%

Emerging Challenges of Radiation-Associated Cardiovascular Dysfunction (RACVD) in Modern Radiation Oncology: Clinical Practice, Bench Investigation, and Multidisciplinary Care

Lee

Liu

Hung

et al. 2020

Front. Cardiovasc. Med.

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Radiotherapy (RT) is a crucial treatment modality in managing cancer patients. However, irradiation dose sprinkling to tumor-adjacent normal tissues is unavoidable, generating treatment toxicities, such as radiation-associated cardiovascular dysfunction (RACVD), particularly for those patients with combined therapies or pre-existing adverse features/comorbidities. Radiation oncologists implement several efforts to decrease heart dose for reducing the risk of RACVD. Even applying the deep-inspiration breath-hold (DIBH) technique, the risk of RACVD is though reduced but still substantial. Besides, available clinical methods are limited for early detecting and managing RACVD. The present study reviewed emerging challenges of RACVD in modern radiation oncology, in terms of clinical practice, bench investigation, and multidisciplinary care. Several molecules are potential for serving as biomarkers and therapeutic targets. Of these, miRNAs, endogenous small non-coding RNAs that function in regulating gene expression, are of particular interest because low-dose irradiation, i.e., 200 mGy (one-tenth of conventional RT daily dose) induces early changes of pro-RACVD miRNA expression. Moreover, several miRNAs, e.g., miR-15b and miR21, involve in the development of RACVD, further demonstrating the potential bio-application in RACVD. Remarkably, many RACVDs are late RT sequelae, characterizing highly irreversible and progressively worse. Thus, multidisciplinary care from oncologists and cardiologists is crucial. Combined managements with commodities control (such as hypertension, hypercholesterolemia, and diabetes), smoking cessation, and close monitoring are recommended. Some agents show abilities for preventing and managing RACVD, such as statins and angiotensin-converting enzyme inhibitors (ACEIs); however, their real roles should be confirmed by further prospective trials.

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The role of MiRNA in polycystic ovary syndrome (PCOS)

Cited by 111 publications

References 65 publications

The Effect of Acupuncture on MiRNA Expression Profiles in the Ovaries of Rats with PCOS and Insulin Resistance

The Effect of Acupuncture on MiRNA Expression Profiles in the Ovaries of Rats with PCOS and Insulin Resistance

The role of miRNAs in regulating adrenal and gonadal steroidogenesis

Emerging Challenges of Radiation-Associated Cardiovascular Dysfunction (RACVD) in Modern Radiation Oncology: Clinical Practice, Bench Investigation, and Multidisciplinary Care

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