The role of miR-7 as a potential switch in the mouse hypothalamus-pituitary-ovary axis through regulation of gonadotropins

He, Jing; Xu, Shirong; Ji, Zengjun; Sun, Yan; Cai, Bingyan; Zhang, Shanhui; Wang, Pingping

doi:10.1016/j.mce.2020.110969

Cited by 11 publications

(11 citation statements)

References 35 publications

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“…The effect of estrogens, its metabolites, and testosterone have different effects on the development of males' and females' brains (Negri-Cesi et al, 2004;He et al, 2012). Previously, researchers found high expression of miR-7 in the pituitary gland of mice and pigs (Li et al, 2011;He et al, 2020). In sows, miR-7 inhibits the synthesis and secretion of Follicle-Stimulating hormones (He et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis of brain and gonad reveals reproduction-related miRNAs in the giant prawn, Macrobrachium rosenbergii

et al. 2022

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Macrobrachium rosenbergii (M. rosenbergii), as a species of common prawn, is a delicacy that is consumed all over the world. By interacting with the target gene 3′-untranslated region (3'-UTR), microRNAs (miRNAs) regulate its expression and ultimately participate in the regulation of reproductive development. However, research focusing on miRNA regulation during gonadal development in M. rosenbergii received very little attention. To explore the association between miRNA and reproduction, we performed RNA sequencing (RNA-seq) on brain and gonad organs in male and female M. rosenbergii. A total of 494 miRNAs were obtained in RNA-seq, including 31 and 59 differentially expressed (DE) miRNAs in the brain and gonads, respectively. Furthermore, 9 DE miRNAs were randomly selected from the brain and gonads, and qRT-PCR was conducted to validate the results of RNA-seq. Interestingly, dpu-miR-133 was found to be substantially expressed in the male brain and testis but poorly expressed in the female brain, ovary, and other organs. Analysis of dpu-miR-133 by Targetscan and MiRanda predicted to target 5-HT1. Furthermore, the dual-luciferase reporter assay manifested that dpu-miR-133 can combine with 5-HT1. Overall, our research work provides basic data for further study on the miRNA-mediated regulation of brain, gonad, and reproductive development of study M. rosenbergii.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis of brain and gonad reveals reproduction-related miRNAs in the giant prawn, Macrobrachium rosenbergii

et al. 2022

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“…For example, miR-7, miR-9, miR-29, miR-184 has been detected in miRNA-seq identification of other species [57][58][59] . In these miRNAs, miR-7 is a crucial factor in regulating the hypothalamus-pituitary-ovary axis of mice 60 . Moreover, miR-7a2 can regulate sexual maturation and reproductive function 61 .…”

Section: Discussionmentioning

confidence: 99%

Integrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii

Wang

Duan

Wang

et al. 2021

Sci Rep

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Hyriopsis cumingii is an important species for freshwater pearl cultivation in China. In terms of pearl production, males have larger pearls and better glossiness than females, but there are few reports focusing on the sex of H. cumingii. In this study, six mRNA and six microRNA (miRNA) libraries were prepared from ovaries and testes. Additionally, 28,502 differentially expressed genes (DEGs) and 32 differentially expressed miRNAs (DEMs) were identified. Compared with testis, 14,360 mRNAs and 20 miRNAs were up-regulated in ovary, 14,142 mRNAs and 12 miRNAs were down-regulated. In DEGs, the known genes related to sex determinism and/or differentiation were also identified, such as DMRT1, SOX9, SF1 for males, FOXL2 for females, and other potentially significant candidate genes. Three sex-related pathways have also been identified, which are Wnt, Notch, and TGF-beta. In 32 DEMs, the three miRNAs (miR-9-5p, miR-92, miR-184) were paid more attention, they predicted 28 target genes, which may also be candidates for sex-related miRNAs and genes. Differential miRNAs target genes analysis reveals the pathway associated with oocyte meiosis and spermatogenesis. Overall, the findings of the study provide significant insights to enhance our understanding of sex differentiation and/or sex determination mechanisms for H. cumingii.

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“…The multivariate regression analysis exhibited that the six-miRNA signature was independent prognostic factor associated with PCOS after adjusting for body mass index (BMI) (adjusted OR 5. 21 2a-f). However, the AUC value of miR-NAs was increased to 0.881 [0.61; 0.92], p = 0.001 when combined in multivariate analysis.…”

Section: Altered Mirnas Expression In Human Ff Among Pcos and Normal Respondersmentioning

confidence: 97%

“…Earlier studies exhibited that miR-320a is of the top ten highly expressed miRNAs identified in the FF samples and positively linked with embryo quality [18,19]. Several studies have established that miR-7-5p acts as a key mediator in the feedback loop of the hypothalamus-pituitary-ovary axis by regulating gonadotropins, and knockout leads to infertility due to hypogonadism in the mouse model [20][21][22]. Previous evidence from in vitro study demonstrated that miR-212 has a potential role in regulating gonadotropins, including follicular development and ovulation in equines [23].…”

Section: Introductionmentioning

confidence: 99%

Extracellular microRNAs: key players to explore the outcomes of in vitro fertilization

Khan¹,

Bhatti

Abbas³

et al. 2021

Reprod Biol Endocrinol

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Background MicroRNAs (miRNAs) are small RNA molecules that modulate post-transcriptional gene regulation. They are often used as promising non-invasive biomarkers for the early diagnosis of cancer. However, their roles in assisted reproduction are still unknown. Methods This prospective study was designed to evaluate the expression profiles of seven extracellular miRNAs (miR-7-5p, miR-202-5p, miR-378-3p, miR-224, miR-320a, miR-212-3p, and miR-21-5p) in human follicular fluid (FF) to explore the outcomes of in vitro fertilization (IVF). Of 255 women, 145 were without polycystic ovary syndrome (PCOS), and their ovarian assets were normal (NOR), while 110 were with normo-androgenic PCOS. Results The combination of six FF miRNAs expression profile discriminated between PCOS and NOR women with a sensitivity of 79.2% and a specificity of 87.32% (AUC = 0.881 [0.61; 0.92], p = 0.001). MiR-202-5p significantly had a lower abundance level, and miR-378-3p had a high abundance level in pooled FF samples from patients treated with human menopausal gonadotropin (hMG) than those treated with recombinant follicle-stimulating hormone (rFSH) (p < 0.001). Our results showed that miRNA-320a was significantly different in top-quality embryos versus non-top-quality embryos on day 3 in NOR patients with a sensitivity of 80% and specificity of 71%, (AUC = [0.753 (0.651; 0.855)], p = 0.001). For clinical pregnancy outcome prediction, FF miRNA-21 exhibited high sensitivity (74.8%) and specificity (83.7%) with the AUC value of 0.774 (0.682; 0.865). Conclusion Conclusively, our results provide evidence that miR-7-5p, miR-378-3p, miR-224, miR-212-3p were a differentially high expression in normo-androgenic PCOS patients than NOR patients. While miRNA-320a was significantly different in top-quality embryos versus non-top-quality embryos on day 3 (p = 0.001). The expression level of FF miR-212-3p was significantly related to the probability of embryos to develop into a high-quality blastocyst in patients with normal ovarian reserve.

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The role of miR-7 as a potential switch in the mouse hypothalamus-pituitary-ovary axis through regulation of gonadotropins

Cited by 11 publications

References 35 publications

Comparative transcriptome analysis of brain and gonad reveals reproduction-related miRNAs in the giant prawn, Macrobrachium rosenbergii

Comparative transcriptome analysis of brain and gonad reveals reproduction-related miRNAs in the giant prawn, Macrobrachium rosenbergii

Integrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii

Extracellular microRNAs: key players to explore the outcomes of in vitro fertilization

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