The role of miR-19b in the inhibition of endothelial cell apoptosis and its relationship with coronary artery disease

Tang, Yong; Zhang, Yachen; Chen, Yu; Yin, Xiaoxing; Shen, Chengxing; Li, Yi‐Gang

doi:10.1038/srep15132

Cited by 62 publications

(52 citation statements)

References 40 publications

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“…miR-495 targets CCL2, significantly promoting human umbilical vein endothelial cell (HUVEC) proliferation and inhibiting apoptosis by affecting the expression of cleaved caspase-3 [19]. In addition, miR-19b plays a key role in the attenuation of TNF- α -induced EC apoptosis, and this function is closely linked to the Apaf1/caspase-7-dependent pathway [22]. Nevertheless, miR-132 promoted apoptosis of HUVEC induced by TNF- α and inhibited its proliferation, viability, and migration by inhibiting SIRT1 [23].…”

Section: Mirnas and Vascular Agingmentioning

confidence: 99%

Function, Role, and Clinical Application of MicroRNAs in Vascular Aging

Lin

Zhan

Wang

et al. 2016

BioMed Research International

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Vascular aging, a specific type of organic aging, is related to age-dependent changes in the vasculature, including atherosclerotic plaques, arterial stiffness, fibrosis, and increased intimal thickening. Vascular aging could influence the threshold, process, and severity of various cardiovascular diseases, thus making it one of the most important risk factors in the high mortality of cardiovascular diseases. As endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) are the main cell biological basis of these pathology changes of the vasculature, the structure and function of ECs and VSMCs play a key role in vascular aging. MicroRNAs (miRNAs), small noncoding RNAs, have been shown to regulate the expression of multiple messenger RNAs (mRNAs) posttranscriptionally, contributing to many crucial aspects of cell biology. Recently, miRNAs with functions associated with aging or aging-related diseases have been studied. In this review, we will summarize the reported role of miRNAs in the process of vascular aging with special emphasis on EC and VSMC functions. In addition, the potential application of miRNAs to clinical practice for the diagnosis and treatment of cardiovascular diseases will also be discussed.

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Section: Mirnas and Vascular Agingmentioning

confidence: 99%

Function, Role, and Clinical Application of MicroRNAs in Vascular Aging

Lin

Zhan

Wang

et al. 2016

BioMed Research International

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“…These RNAs are involved in the regulation of multiple cellular processes, including proliferation, differentiation, development, and apoptosis, [8] and have also been associated with AS [9–11] and autophagy [12, 13]. …”

Section: Introductionmentioning

confidence: 99%

miR-155 Promotes ox-LDL-Induced Autophagy in Human Umbilical Vein Endothelial Cells

Zhang

Pan

Yang

et al. 2017

Mediators of Inflammation

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As an evolutionarily conserved metabolic process, autophagy is involved in the process of atherosclerosis (AS). MicroRNA-155 (miR-155), a multifunctional miRNA, plays an important role in many physiological and pathological conditions, including AS and autophagy. However, the effect of miR-155 on the regulation of autophagy in endothelial cells has not been reported to date. Therefore, the objective of our study was to investigate the role of miR-155 in autophagy induced by oxidized low-density lipoprotein (ox-LDL) in human umbilical vein endothelial cells (HUVECs). Our results demonstrated that ox-LDL induced autophagy in HUVECs and increased the expression of miR-155 significantly. Overexpression of miR-155 improved autophagic activity, whereas low expression of miR-155 inhibited autophagic activity. Therefore, the data demonstrated that miR-155 has a modulating effect on the autophagy of vascular endothelial cells.

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“…Previous studies indicate that miRNAs directly affect radioresistance by interfering with specific pathways [4], including sensing DNA damage [5], repair of DNA double-strand breaks (DSBs) [6], cell cycle checkpoint activation [7], formation of reactive oxygen species [8] and apoptosis [9], and autophagy [10]. miR-19b-3p belongs to both the miR-17-92 and miR-106-363 clusters, which play significant roles in proliferation and cell survival [11, 12]. In particular, miR-19b-3p has been validated as an oncogene in several human malignancies [12].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-19b-3p regulates nasopharyngeal carcinoma radiosensitivity by targeting TNFAIP3/NF-κB axis

Huang

et al. 2016

J Exp Clin Cancer Res

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BackgroundNasopharyngeal carcinoma (NPC) is among the most common squamous cell carcinoma in South China and Southeast Asia. Radiotherapy is the primary treatment for NPC. However, radioresistance acts as a significant factor that limits the efficacy of radiotherapy for NPC patients. Growing evidence supports that microRNAs (miRNAs) play an important role in radiation response.MethodsReal-time quantitative PCR was used to analyze the expression of miR-19b-3p in NPC cell lines and NP69. miR-19b-3p expression profiles in NPC tissues were obtained from the Gene Expression Omnibus database. The effect of miR-19b-3p on radiosensitivity was evaluated by cell viability assays, colony formation assays and in vivo experiment. Apoptosis and cell cycle were examined by flow cytometry. Luciferase reporter assay was used to assess the target genes of miR-19b-3p. Expression of target proteins and downstream molecules were analyzed by Western blot.ResultsmiR-19b-3p was upregulated in NPC and served as an independent predictor for reduced patient survival. Radioresponse assays showed that miR-19b-3p overexpression resulted in decreased sensitivity to irradiation, whereas miR-19b-3p downregulation resulted in increased sensitivity to irradiation in vitro. Moreover, miR-19b-3p decreased the sensitivity of NPC cells to irradiation in vivo. Luciferase reporter assay confirmed that TNFAIP3 was a direct target gene of miR-19b-3p. Knockdown of TNFAIP3 reduced sensitivity to irradiation, whereas upregulation of TNFAIP3 expression reversed the inhibitory effects of miR-19b-3p on NPC cell radiosensitivity. Mechanistically, we found that miR-19b-3p increased NPC cell radioresistance by activating the TNFAIP3/ NF-κB axis.ConclusionsmiR-19b-3p contributes to the radioresistance of NPC by activating the TNFAIP3/ NF-κB axis. miR-19b-3p is a determinant of NPC radioresponse and may serve as a potential therapeutic target in NPC treatment.Electronic supplementary materialThe online version of this article (doi:10.1186/s13046-016-0465-1) contains supplementary material, which is available to authorized users.

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The role of miR-19b in the inhibition of endothelial cell apoptosis and its relationship with coronary artery disease

Cited by 62 publications

References 40 publications

Function, Role, and Clinical Application of MicroRNAs in Vascular Aging

Function, Role, and Clinical Application of MicroRNAs in Vascular Aging

miR-155 Promotes ox-LDL-Induced Autophagy in Human Umbilical Vein Endothelial Cells

MicroRNA-19b-3p regulates nasopharyngeal carcinoma radiosensitivity by targeting TNFAIP3/NF-κB axis

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