The role of microRNAs in corneal neovascularization and its relation to VEGF

Yaşar, Mimbay; Çakmak, Harun; Dündar, Sema Oruç; Boyacioglu, Sedat; Caliskan, Metin; Ergin, Kemal

doi:10.1080/15569527.2020.1813749

Cited by 3 publications

(2 citation statements)

References 36 publications

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“…The role of miR-21 in CNV has been reported ( Yasar et al, 2020 ). In this study, they have demonstrated that VEGF expression is significantly increased in the corneal samples with CNV, compare with the controls.…”

Section: Discussionmentioning

confidence: 99%

Targeting HMGB1-NFκb Axis and miR-21 by Glycyrrhizin: Role in Amelioration of Corneal Injury in a Mouse Model of Alkali Burn

et al. 2022

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Corneal neovascularization (CNV) is a sight-threatening condition usually associated with various inflammatory settings including chemical injury. High mobility group box 1 (HMGB1) is identified as an inflammatory alarmin in diverse tissue damage. Here, we evaluate the expression of HMGB1 and the consequences of its inhibition through its selective inhibitor glycyrrhizin (GLY) in alkali burn-induced corneal inflammation and neovascularization. GLY effectively attenuated alkali burn-induced HMGB1 expression at both mRNA and protein levels. Furthermore, slit-lamp analysis, ink perfusion, H&E staining, and CD31 histochemical staining showed that GLY relieved corneal neovascularization, while GLY attenuated VEGF expression via inhibiting HMGB1/NF-κB/HIF-1α signal pathway. In addition, GLY treatment decreased the cytokine expression of CCL2 and CXCL5, accompanied by the reduction of their receptors of CCR2 and CXCR2. GLY diminished the inflammatory cell infiltration of the cornea, as well as reduced the expression of IL-1β, IL-6, and TNF-α. Moreover, treatment with GLY reduced the degree of cornea opacity through inactivating extracellular HMGB1 function, which otherwise induces TGF-β1 release and myofibroblast differentiation. Furthermore, we found that GLY treatment attenuated the upregulation of miR-21 levels in alkali burned cornea; while inhibition of miR-21in keratocytes in vitro, significantly inhibited TGF-β1-induced myofibroblast differentiation. Collectively, our results suggested that targeting HMGB1-NFκb axis and miR-21 by GLY could introduce a therapeutic approach to counter CNV.

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Section: Discussionmentioning

confidence: 99%

Targeting HMGB1-NFκb Axis and miR-21 by Glycyrrhizin: Role in Amelioration of Corneal Injury in a Mouse Model of Alkali Burn

et al. 2022

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“…After 48 hours of the second injection of LA, thiopental sodium 50 mg/kg subcutaneous injections were used to anesthetize the rats before they were sacrificed by cervical dislocation [20] . After being removed from the body, the pancreas was fixed in a 10 % formalin solution.…”

Section: Histological Studymentioning

confidence: 99%

The Beneficial Effects of Lipoic Acid Versus Coenzyme Q10 on Arginine-Induced Acute Pancreatitis in Albino Rats: Histological and Immunohistochemical study

Elsyade

Fattah²,

Khaled³

et al. 2021

Journal of Medical Histology

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Background: Acute pancreatitis (AP) is a self-limiting condition to a widespread, deadly multi-organ failure that can result in death. Objectives: To compare the protective effect of alpha lipoic acid (ALA) and Coenzyme (Q10) in an experimental rat model of L-arginine-induced AP. Materials (Subjects): Forty adult male albino rats were randomized equally into 4 groups: (i) The control group; (ii) The AP group received L-Arginine by intraperitoneal (IP) injection in two doses of 2 g/kg body weight each, 1 hour apart, (iii) The ALA+AP group was injected with L-Arginine and intramuscular (IM) injection of ALA in two doses of 60mg/kg body weight; 1 hour before L-arginine injection and 24 hours after the second dose of L-arginine; (iii) The Coenzyme Q10+AP group was injected with L-Arginine and IM injection of Q10 in two doses of 30mg/kg body weight; 1 hour before L-arginine injection and 24 hours after the second dose of L-arginine. Histological, immunohistochemical for HSP70 and NF-κB, and morphometric studies were done followed by statistical analysis. Results: AP group showed significant distortion of the pancreatic acini with obvious cellular damage while those treated by ALA and Coenzyme Q 10 especially ALA showed significant pancreatic tissue improvement as shown by HSP70 and NF-κB immunoexpressing. Conclusions: ALA and Q 10 therapy proved a clear protective effect in suppressing experimentally generated AP in male albino rats, but the improvement was more obvious with ALA.

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