The role of microRNAs in embryonic stem cell and induced pluripotent stem cell differentiation in male germ cells

Mahabadi, Javad Amini; Sabzalipoor, Hamed; Nikzad, Hossein; Seyedhosseini, Elahe; Enderami, Seyed Ehsan; Hayat, Seyed Mohammad Gheibi; Sahebkar, Amirhosein

doi:10.1002/jcp.27990

Cited by 16 publications

(9 citation statements)

References 114 publications

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“…miRNAs are small noncoding RNAs that are evolutionarily conserved, and they regulate gene expression at the posttranscriptional level by interacting with the 3'UTR of mRNAs and recruiting molecular machinery that degrades the target mRNAs (22). Research on miRNAs is currently of great importance in species ranging from plants (23) to humans, especially in the fields of ontogeny (24), cancer (25), and inflammation (26,27). In recent years, many studies have contributed to the discovery of novel miRNAs; although these works were intended to shed light on the regulatory roles of these molecules, the confirmation of miRNA-mRNA target pairs has mainly been accomplished based upon interaction or expression patterns but not functional targeting (28).…”

Section: Introductionmentioning

confidence: 99%

Stimulation of Mytilus galloprovincialis Hemocytes With Different Immune Challenges Induces Differential Transcriptomic, miRNomic, and Functional Responses

et al. 2020

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Mediterranean mussels (Mytilus galloprovincialis) are marine bivalve molluscs with high resilience to biotic and abiotic stress. This resilience is one of the reasons why this species is such an interesting model for studying processes such as the immune response. In this work, we stimulated mussel hemocytes with poly I:C, β-glucans, and LPS and then sequenced hemocyte mRNAs (transcriptome) and microRNAs (miRNome) to investigate the molecular basis of the innate immune responses against these pathogen-associated molecular patterns (PAMPs). An immune transcriptome comprising 219,765 transcripts and an overview of the mussel miRNome based on 5,175,567 non-redundant miRNA reads were obtained. The expression analyses showed opposite results in the transcriptome and miRNome; LPS was the stimulus that triggered the highest transcriptomic response, with 648 differentially expressed genes (DEGs), while poly I:C was the stimulus that triggered the highest miRNA response, with 240 DE miRNAs. Our results reveal a powerful immune response to LPS as well as activation of certain immunometabolism- and ageing/senescence-related processes in response to all the immune challenges. Poly I:C exhibited powerful stimulating properties in mussels, since it triggered the highest miRNomic response and modulated important genes related to energy demand; these effects could be related to the stronger activation of these hemocytes (increased phagocytosis, increased NO synthesis, and increased velocity and accumulated distance). The transcriptome results suggest that after LPS stimulation, pathogen recognition, homeostasis and cell survival processes were activated, and phagocytosis was induced by LPS. β-glucans elicited a response related to cholesterol metabolism, which is important during the immune response, and it was the only stimulus that induced the synthesis of ROS. These results suggest a specific and distinct response of hemocytes to each stimulus from a transcriptomic, miRNomic, and functional point of view.

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Section: Introductionmentioning

confidence: 99%

Stimulation of Mytilus galloprovincialis Hemocytes With Different Immune Challenges Induces Differential Transcriptomic, miRNomic, and Functional Responses

et al. 2020

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“…Interestingly, miRNAs can modulate/deregulate the expression and functioning of genes and their products associated with the signaling pathways which are integral for the maintenance, growth, and function of CSCs such as Wnt/β-catenin, Notch, and others [50]. It has now become evident that miRNAs play a vital role in various developmental and regulatory mechanisms of stem cells such as maintenance, reprogramming, pluripotency, and differentiation by targeting various differentiation pathways, and thus, the overall regulatory action of miRNAs on stem cells suggests a critical role of miRNAs in the development of mammals [2,51].…”

Section: Role Of Mirna and Oncogenic Signaling Pathways In Human Cancmentioning

confidence: 99%

“…reprogramming, pluripotency, and differentiation by targeting various differentiation pathways, and thus, the overall regulatory action of miRNAs on stem cells suggests a critical role of miRNAs in the development of mammals [2,51]. Initially, it was the discovery of let-7 and lin-4 miRNA in Caenorhabditis elegans which revealed that miRNAs play a significant role in regulating the various molecular mechanisms related to the embryonic development and function of stem cells in mammals.…”

Section: Role Of Mirna and Oncogenic Signaling Pathways In Human Cancmentioning

confidence: 99%

Role of miRNA-Regulated Cancer Stem Cells in the Pathogenesis of Human Malignancies

Khan

Ahmed

Elareer

et al. 2019

Cells

227

168

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Recent biomedical discoveries have revolutionized the concept and understanding of carcinogenesis, a complex and multistep phenomenon which involves accretion of genetic, epigenetic, biochemical, and histological changes, with special reference to MicroRNAs (miRNAs) and cancer stem cells (CSCs). miRNAs are small noncoding molecules known to regulate expression of more than 60% of the human genes, and their aberrant expression has been associated with the pathogenesis of human cancers and the regulation of stemness features of CSCs. CSCs are the small population of cells present in human malignancies well-known for cancer resistance, relapse, tumorigenesis, and poor clinical outcome which compels the development of novel and effective therapeutic protocols for better clinical outcome. Interestingly, the role of miRNAs in maintaining and regulating the functioning of CSCs through targeting various oncogenic signaling pathways, such as Notch, wingless (WNT)/β-Catenin, janus kinases/ signal transducer and activator of transcription (JAK/STAT), phosphatidylinositol 3-kinase/ protein kinase B (PI3/AKT), and nuclear factor kappa-light-chain-enhancer of activated B (NF-kB), is critical and poses a huge challenge to cancer treatment. Based on recent findings, here, we have documented the regulatory action or the underlying mechanisms of how miRNAs affect the signaling pathways attributed to stemness features of CSCs, such as self-renewal, differentiation, epithelial to mesenchymal transition (EMT), metastasis, resistance and recurrence etc., associated with the pathogenesis of various types of human malignancies including colorectal cancer, lung cancer, breast cancer, head and neck cancer, prostate cancer, liver cancer, etc. We also shed light on the fact that the targeted attenuation of deregulated functioning of miRNA related to stemness in human carcinogenesis could be a viable approach for cancer treatment.

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“…The aberrant expression of microRNAs leads to various cancers [6,7]. MicroRNAs regulate the development of cancers, such as migration, apoptosis, proliferation and differentiation [8][9][10]. MiR-301a-3p expresses in many tissues, which belongs to miR-301 family.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA (MiR)-301a-3p regulates the proliferation of esophageal squamous cells via targeting PTEN

Zhang

Liu

2020

Bioengineered

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Human esophageal carcinoma (EC) is a common cancer, which leads to many deaths worldwide every year. Our study aimed to explore the mechanism of miR-301a-3p regulating the proliferation of esophageal squamous cell carcinoma (ESCC) cells. We had collected ESCC tissues and adjacent normal esophageal tissues from 47 patients. The relative levels of miR-301a-3p/U6 in ESCC tissues and cells were analyzed by real-time PCR. And we measured the relative protein levels of PTEN, BCL-2, BAX, and p-AKT/AKT by Western blot. Eca-109 cell proliferation was detected by MTT assay and colony formation. Compared with adjacent normal esophageal tissues, the relative level of miR-301a-3p/U6 was elevated in ESCC tissues. MiR-301a-3p could facilitate ESCC cell proliferation. And miR-301a-3p directly bind to PTEN 3ʹ-UTR and negatively regulated PTEN protein expression. Moreover, silencing PTEN could reversed inhibited proliferation of Eca-109 cells induced by miR-301a-3p inhibitor, while overexpression PTEN could reversed enhanced proliferation of Eca-109 cells induced by miR-301a-3p mimic. Taken together, miR-301a-3p promoted ESCC cell proliferation by supressing PTEN.

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The role of microRNAs in embryonic stem cell and induced pluripotent stem cell differentiation in male germ cells

Cited by 16 publications

References 114 publications

Stimulation of Mytilus galloprovincialis Hemocytes With Different Immune Challenges Induces Differential Transcriptomic, miRNomic, and Functional Responses

Stimulation of Mytilus galloprovincialis Hemocytes With Different Immune Challenges Induces Differential Transcriptomic, miRNomic, and Functional Responses

Role of miRNA-Regulated Cancer Stem Cells in the Pathogenesis of Human Malignancies

MicroRNA (MiR)-301a-3p regulates the proliferation of esophageal squamous cells via targeting PTEN

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