The role of microRNAs in mammalian oocytes and embryos

Hossain, Munir; Salilew‐Wondim, Dessie; Schellander, K.; Tesfaye, Dawit

doi:10.1016/j.anireprosci.2012.08.009

Cited by 59 publications

(43 citation statements)

References 118 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A broad range of biological processes, such as cell differentiation, proliferation and apoptosis, tissue morphogenesis, fertility, metabolism, and immune response, have been shown to be regulated by miRNAs (Ambros, 2004;Plasterk, 2006;Shivdasani, 2006;Sun et al, 2012;Hossain et al, 2012a). As a major regulator of gene expression, several studies have used Solexa sequencing to demonstrate that miRNAs play specific roles in ovarian function and ovulation (Baley and Li, 2012;Hossain et al, 2012b).…”

Section: Introductionmentioning

confidence: 99%

Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing

Shen¹,

Chen²,

Jia³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The role of microRNAs (miRNAs) in the regulation of mammalian reproduction has been demonstrated previously. However, only a few studies have assessed the role of miRNAs in the reproduction processes of sheep. The elucidation of miRNA expression profiles in the ovaries of different sheep breeds representing fecundity extremes will be useful in understanding the roles of miRNAs in sheep reproduction. In this study, two small RNA libraries were constructed from ovary tissue taken from Qira black sheep and Hetian sheep during the estrous period and then sequenced using the Solexa sequencing method. We obtained 9,565,212 and 9,563,426 high-quality reads from Qira black sheep and Hetian sheep, respectively. In total, 531 miRNAs, including 98 putative miRNAs, were identified. Among the conserved miRNAs, 125 known miRNAs were significantly differentially expressed in the Qira black sheep and Hetian sheep libraries, with 24 upregulated and 101 downregulated in the Hetian sheep compared to the Qira black sheep. Four differentially expressed miRNAs were analyzed using real-time 7357 miRNA expression analysis in sheep ovaries ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 7356-7367 (2015) quantitative PCR to validate the reliability of the Solexa sequencing results. These results provide a foundation for future research on the regulation of miRNAs in sheep fertility and enrich the sheep miRNA databases.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing

Shen¹,

Chen²,

Jia³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…In fact, miRNAs are emerging as a group of gene-expression modulators critically involved in embryo implantation (Chakrabarty et al 2007, Hu et al 2008, Nagaraja et al 2008, Revel et al 2011. They have been shown to exhibit dynamic temporal and spatial expression in both the uterus and the blastocyst during implantation (Hossain et al 2012, Su et al 2014. Interestingly, two miRNAs predicted to target COX2, miR-101a and miR-199a-3p, have also been reported to be spatiotemporally expressed in the uterus which coincides with the expression of COX2 during implantation in mice, suggesting the potential role of these miRNAs in the process (Chakrabarty et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Regulation of miR-101/miR-199a-3p by the epithelial sodium channel during embryo implantation: involvement of CREB phosphorylation

Sun¹,

Ruan²,

Guo³

et al. 2014

Reproduction

View full text Add to dashboard Cite

In our previous study, we have demonstrated that the epithelial sodium channel (ENaC) mediates the embryo-derived signals leading to the activation of CREB and upregulation of cyclooxygenase type 2 (COX2) required for embryo implantation. This study aims to investigate whether microRNAs (miRNAs) are involved in the ENaC-induced upregulation of COX2 during embryo implantation. The results show that the levels of miR-101 and miR-199a-3p, two COX2 targeting miRNAs, are reduced by ENaC activation, and increased by ENaC inhibition or knock-down of ENaC subunit (ENaCa) in human endometrial surface epithelial (HES) cells or in mouse uteri during implantation. Phosphorylation of CREB is induced by the activation of ENaC, and blocked by ENaC inhibition or knockdown in HES cells. Knockdown of ENaCa or CREB in HES cells or in mouse uterus in vivo results in increases in miR-101 and miR-199a-3p, accompanied with decreases in COX2 protein levels and reduction in implantation rate. The downregulation of COX2 caused by knockdown of ENaC or CREB can be recovered by the inhibitors of miR-101 or miR-199a-3p in HES cells. These results reveal a novel molecular mechanism modulating COX2 expression during embryo implantation via ENaC-dependent CREB activation and COX2-targeting miRNAs.

show abstract

“…The miRNAs are encoded within the intragenic regions (49) and within intronic regions (50) . Those encoded in intragenic regions are expressed under the action of RNApol II and referred to as pri-miRNAs (primary miRNA); these are processed by a complex of ribonucleic protein, the enzyme DROSHA and protein DGCR8 that cut to the pri-miRNA and leave it in structure of stem-bubble (51,52) .…”

Section: Role Of the Small Rnas During The Oogenesismentioning

confidence: 99%

“…Los miRNAs están codificados dentro de regiones intergénicas (49) y dentro de regiones intrónicas (50) . Los que están codificados en regiones intergénicas se expresan bajo la acción de la RNApol II y se denominan pri-miRNAs (miRNA primario), estos son procesados por un complejo ribonucleico proteico, compuesto por la enzima DROSHA y la proteína DGCR8 que cortan al pri-miRNA y lo dejan en estructura de tallo burbuja (51,52) .…”

Section: Papel De Los Rnas Pequeños Durante La Ovogénesisunclassified

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

Burrola-Barraza¹,

González-Rodríguez

2015

Rev. Mex. Cienc. Pecu.

View full text Add to dashboard Cite

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos. RevisiónEffects of the maternal mRNA on the maturation of the oocyte and early embryonic development in mammals. Review M. Eduviges Burrola-Barraza a , Everardo González-Rodríguez a RESUMENObtener ovocitos madurados in vitro, que sean competentes para fertilizarse y originar un porcentaje superior al 50 % de blastocitos viables, es una de las principales metas que existen en el desarrollo in vitro de embriones bovinos. Es por ello necesario entender los procesos celulares, que desembocan en la maduración del ovocito durante la foliculogénesis y su subsecuente transición al embrión. La transición del ovocito al embrión es un proceso complejo que involucra la inactivación genómica del ovocito y la activación del genoma embrionario. Este proceso se da en bovinos en la etapa de 8 a 16 células y presenta una degradación selectiva de RNAm maternos, que fueron almacenados durante la ovogénesis y son la fuente para la codificación de proteínas en las etapas iníciales del desarrollo embrionario. La acción de los RNAm maternos es clave para que la activación del genoma embrionario se realice en tiempo y forma adecuados. Entender la participación de estos transcritos en la activación del genoma embrionario, es esencial para esclarecer los procesos celulares que fallan en los protocolos de maduración de ovocitos in vitro. Es por esto que el objetivo de esta revisión fue recopilar la información de los principales RNAm maternos que han sido identificados tanto en el modelo murino como el bovino, esto con el fin de integrar el conocimiento relacionado con los procesos genómicos que permiten el desarrollo del embrión en el bovino, y diseñar nuevas estrategias que permitan mejorar los protocolos de fertilización in vitro.PALABRAS CLAVE: Ovocito, Activación genoma embrionario, Bovino. ABSTRACTGet in vitro matured oocytes that could be competent for in vitro fertilization with a result of 50 % viable blastocysts, is one of the main problems in the in vitro development of bovine embryos. For these reason, is necessary to understand the cellular processes that lead to the maturation of the oocyte during folliculogenesis and subsequent transition to the embryo. The transition from oocyte to embryo is a complex process that involves genomic inactivation of the oocyte and embryonic genome activation. This process occurs in cattle in the stage of 8 to 16 cells where there are a selective degradation of maternal mRNA, which were stored during oogenesis and are the source of protein in the early stages of embryonic development. The action of maternal mRNA is a key for that embryonic genome activation takes place in form and time. Understanding the involvement of these transcripts in embryonic genome activation is essential to elucidate the cellular processes falling in oocyte maturation in vitro protocols. The aim of this review was to gather information from maternal genes that have been isolated both in the murine model and c...

show abstract

The role of microRNAs in mammalian oocytes and embryos

Cited by 59 publications

References 118 publications

Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing

Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing

Regulation of miR-101/miR-199a-3p by the epithelial sodium channel during embryo implantation: involvement of CREB phosphorylation

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

Contact Info

Product

Resources

About