The Role of Metabolomics in Systems Biology

Greef, J. van der; Davidov, Eugene; Verheij, Elwin; Vogels, Jack; Heijden, Rob van der; Adourian, Aram; Orešič, Matej; Marple, Edward W.; Naylor, Stephen

doi:10.1007/978-1-4615-0333-0_10

Cited by 49 publications

(32 citation statements)

References 59 publications

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“…NMR is routinely being applied to monitor changes in the composition and concentration of metabolites found in biofl uids and cell extracts to: (1) monitor drug toxicity [4], [5], [6] and [7], (2) identify disease markers [4], [8], [9], [10] and [11] and (3) explore in vivo protein function and activity [3], [12], [13] and [14]. 1 H NMR spectrum collected on the entire metabolome obtained from whole cell lysis or biofl uids tend to be extremely complex due to the presence of hundreds of low-molecular weight compounds.…”

Section: Introductionmentioning

confidence: 99%

Negative impact of noise on the principal component analysis of NMR data

Halouska

Powers

2006

Journal of Magnetic Resonance

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Section: Introductionmentioning

confidence: 99%

Negative impact of noise on the principal component analysis of NMR data

Halouska

Powers

2006

Journal of Magnetic Resonance

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“…This type of analysis allows one to simultaneously examine the relative state of individuals according to three or more variables. We used Principal Component Analysis (PCA) statistical methods, which are specially developed, for application in biomedical research using TNO IMPRESS, EQUEST and WINLIN software [21,22].…”

Section: Statistics and Calculations Lc-ms Datamentioning

confidence: 99%

The Relation between Non-adipose Muscle Fat and Hepatic Steatosis Studied with Localized 1H Magnetic Resonance Spectroscopy (1H MRS) and LC-MS Techniques

Vjt¹,

Booms²,

Verheij³

et al. 2016

Anat Physiol

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Aim/objective: In this study we investigated ectopic fat storage in the muscle and the liver using 1 H Magnetic Resonance Spectroscopy ( 1 H-MRS). The inability to store fat in adipose tissue leads to ectopic Triacylglycerol (TG) accumulation in muscle followed by the liver: the so called "overflow hypothesis". It is assumed that when steatosis occurs in organs like the liver we can speak from "Metabolic Syndrome". Methods:We compared the effects of two different diet interventions, 24 h-starvation and 40 days High-fat diet (+0.25% cholesterol and 45% energy from bovine lard) with control mice. Characterization of lipid molecular species in non-adipose muscle homogenate was performed by comparing the groups using liquid chromatography coupled to mass spectrometry (LC-MS) techniques following a Systems Biology lipidomics based approach. Reversed phase liquid chromatography coupled to mass spectrometry (LC-MS) were used to quantify and qualify the rearrangement and repartitioning of the triacylglycerol compound in the liver organ. Results:The major message of this manuscript is the interaction of remnant organ/tissue called "carcass" in the absorption capacity of lipids and the spill-over of these lipid compounds (mainly TG's) to the liver. Our data suggest that if the remnant muscle compartment is saturated with lipids until ≈500 g/kg dry matter there is no TGs accumulation in the liver, but above this level there is a spill over in the plasma resulting in fat accumulation in the liver. Conclusion:We demonstrated in this study that fat can be stored in the muscle but when this compartment is saturated the liver takes over the function as a fat sink, the "overflow hypothesis" resulting finally in hepatic steatosis and 'Metabolic Syndrome'.

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“…Only a few systems biology studies in nutrition and medical systems biology covering all three levels are published or are related to cell systems [15], typically a selection is preferred using metabolomics [16,17] and/or proteomics for body fluids or metabolomics and/or transcriptomics in other cases. Sample limitations in nutritional and medical domains is of course not an issue for plant and microbial systems biology studies.…”

Section: Systems Biology In the Life Sciencesmentioning

confidence: 99%

“…Additionally, the use of electrochemical detection for liquid chromatography, in particular the coulometric array technology for instance applied in Moton Neuron Disease [36], has become a substantial component of metabolomics technologies in recent years [37], while other approaches are emerging, for instance capillary electrophoresis combined with mass spectrometry (CE-MS) [38][39][40]. Combinations of different techniques to comprehensively measure the metabolome were developed at the end of the 1990s [17]. In the area of plant metabolomics (not discussed in detail here), major breakthroughs on the technology side have been realized [41].…”

Section: Metabolomicsmentioning

confidence: 99%