The Role of Metabolic Enzymes in the Regulation of Inflammation

Godfrey, Wesley H.; Kornberg, Michael D.

doi:10.3390/metabo10110426

Cited by 14 publications

(6 citation statements)

References 164 publications

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“…Cellular enzymes conduct and catalyze the majority of basic cellular bioreactions and mediate cellular communication and cellular migration, as well as inflammation and cancer. ,,− Preserved enzymatic activities could help to explore the roles of enzymes in the pathogenesis of various diseases − and constitute various classes of enzymatic biocatalysts. To investigate enzymatic activities in silicified cells, a series of commercially sourced biochemical assays (PNPP, MUP, and CM-H2DCFD) were used to analyze alkaline phosphatase, acid phosphatase, and esterase enzymatic activity, respectively (Table S1).…”

Section: Results and Discussionmentioning

confidence: 99%

Robust and Long-Term Cellular Protein and Enzymatic Activity Preservation in Biomineralized Mammalian Cells

et al. 2022

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Preservation of evolved biological structure and function in robust engineering materials is of interest for storage of biological samples before diagnosis and development of vaccines, sensors, and enzymatic reactors and has the potential to avoid cryopreservation and its associated coldchain issues. Here, we demonstrate that "freezing cells in amorphous silica" is a powerful technique for long-term preservation of whole mammalian cell proteomic structure and function at room temperature. Biomimetic silicification employs the crowded protein microenvironment of mammalian cells as a catalytic framework to proximally transform monomeric silicic acid into silicates forming a nanoscopic silica shell over all biomolecular interfaces. Silicification followed by dehydration preserves and passivates proteomic information within a nanoscale thin silica coating that exhibits size selective permeability (<3.6 nm), preventing protein leaching and protease degradation of cellular contents, while providing access of small molecular constituents for cellular enzymatic reaction. Exposure of dehydrated silicified cells to mild etchant or prolonged hydrolysis removes the silica, completely rerevealing biomolecular components and restoring their accessibility and functionality.

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Section: Results and Discussionmentioning

confidence: 99%

Robust and Long-Term Cellular Protein and Enzymatic Activity Preservation in Biomineralized Mammalian Cells

et al. 2022

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“…Although in the field of supramolecular polymers, the delivery of mRNA remains a challenge, there are few works [191,192] dedicated to improve the formulation of stimuli-responsive assembled micelles (that are highly evaluated for their use in human clinical studies [193,194]) to improve their stability for efficient gene transfection. In addition, the activity of enzymes associated with cancer [195] or inflammation-associated disorders [196] can be altered in such human pathologies. Although the development of enzyme-responsive supramolecular polymers remains a challenge, the use of supramolecular drug delivery systems that respond to the altered activity of matrix metalloproteases (MMPs) would be a promising approach to treat inflammatory-related illnesses, including cancer or rheumatoid arthritis disease [197].…”

Section: Response To Internal Stimulimentioning

confidence: 99%

Responsive Supramolecular Polymers for Diagnosis and Treatment

Martínez-Orts,

Pujals

2024

IJMS

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Stimuli-responsive supramolecular polymers are ordered nanosized materials that are held together by non-covalent interactions (hydrogen-bonding, metal-ligand coordination, π-stacking and, host–guest interactions) and can reversibly undergo self-assembly. Their non-covalent nature endows supramolecular polymers with the ability to respond to external stimuli (temperature, light, ultrasound, electric/magnetic field) or environmental changes (temperature, pH, redox potential, enzyme activity), making them attractive candidates for a variety of biomedical applications. To date, supramolecular research has largely evolved in the development of smart water-soluble self-assemblies with the aim of mimicking the biological function of natural supramolecular systems. Indeed, there is a wide variety of synthetic biomaterials formulated with responsiveness to control and trigger, or not to trigger, aqueous self-assembly. The design of responsive supramolecular polymers ranges from the use of hydrophobic cores (i.e., benzene-1,3,5-tricarboxamide) to the introduction of macrocyclic hosts (i.e., cyclodextrins). In this review, we summarize the most relevant advances achieved in the design of stimuli-responsive supramolecular systems used to control transport and release of both diagnosis agents and therapeutic drugs in order to prevent, diagnose, and treat human diseases.

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“…[36,37]. Notably, the capacity of these proteins to develop moonlighting functions has been conserved in mammalian cells [38].…”

Section: Beyond Metabolic Reprogramming: Immune Regulatory Roles Of Metabolic Enzymes and Metabolitesmentioning

confidence: 99%

Role of Cellular Metabolism during Candida-Host Interactions

et al. 2022

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Microscopic fungi are widely present in the environment and, more importantly, are also an essential part of the human healthy mycobiota. However, many species can become pathogenic under certain circumstances, with Candida spp. being the most clinically relevant fungi. In recent years, the importance of metabolism and nutrient availability for fungi-host interactions have been highlighted. Upon activation, immune and other host cells reshape their metabolism to fulfil the energy-demanding process of generating an immune response. This includes macrophage upregulation of glucose uptake and processing via aerobic glycolysis. On the other side, Candida modulates its metabolic pathways to adapt to the usually hostile environment in the host, such as the lumen of phagolysosomes. Further understanding on metabolic interactions between host and fungal cells would potentially lead to novel/enhanced antifungal therapies to fight these infections. Therefore, this review paper focuses on how cellular metabolism, of both host cells and Candida, and the nutritional environment impact on the interplay between host and fungal cells.

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The Role of Metabolic Enzymes in the Regulation of Inflammation

Cited by 14 publications

References 164 publications

Robust and Long-Term Cellular Protein and Enzymatic Activity Preservation in Biomineralized Mammalian Cells

Robust and Long-Term Cellular Protein and Enzymatic Activity Preservation in Biomineralized Mammalian Cells

Responsive Supramolecular Polymers for Diagnosis and Treatment

Role of Cellular Metabolism during Candida-Host Interactions

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