The role of membrane protein sulfhydryl groups in hydrogen peroxide-mediated membrane damage in human erythrocytes

Snyder, Lois; Fortier, Normand L.; Leb, Laszlo; McKenney, James; Trainor, J; Sheerin, Harland E.; Mohandas, Narla

doi:10.1016/0005-2736(88)90245-3

Cited by 64 publications

(27 citation statements)

References 26 publications

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“…Imbalance between ROS production and antioxidant cell defences has been reported to occur in several physiopathological conditions of both animals and man: for example tissue ischemia and reperfusion Many in vitro and in vivo studies showed that several parameters of red blood cell function and integrity are negatively affected by increased oxidative stress. In fact, changes of erythrocyte membrane ionic permeability [7], increase of lipid peroxidation [8], oxidation of protein sulfihydryl groups [9] and activation of proteolysis [10] have all been described following the challenge of red blood cells with different oxygen radical-generating systems. However, data referring to modifications of erythrocyte energy metabolism under these experimental conditions are not abundant, and thus it is not very clear which metabolites, and hence enzymes, are more sensitive to oxidative stress.…”

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confidence: 99%

“…In fact, changes of erythrocyte membrane ionic permeability [7], increase of lipid peroxidation [8], oxidation of protein sulfihydryl groups [9] and activation of proteolysis [10] have all been described following the challenge of red blood cells with different oxygen radical-generating systems. However, data referring to modifications of erythrocyte energy metabolism under these experimental conditions are not abundant, and thus it is not very clear which metabolites, and hence enzymes, are more sensitive to oxidative stress.…”

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confidence: 99%

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Energy metabolism and lipid peroxidation of human erythrocytes as a function of increased oxidative stress

Tavazzi

Pierro

Amorini

et al. 2000

European Journal of Biochemistry

140

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To study the influence of oxidative stress on energy metabolism and lipid peroxidation in erythrocytes, cells were incubated with increasing concentrations (0.5±10 mm) of hydrogen peroxide for 1 h at 37 8C and the main substances of energy metabolism (ATP, AMP, GTP and IMP) and one index of lipid peroxidation (malondialdehyde) were determined by HPLC on cell extracts. Using the same incubation conditions, the activity of AMP-deaminase was also determined. Under nonhaemolysing conditions (at up to 4 mm H 2 O 2 ), oxidative stress produced, starting from 1 mm H 2 O 2 , progressive ATP depletion and a net decrease in the intracellular sum of adenine nucleotides (ATP + ADP + AMP), which were not paralleled by AMP formation. Concomitantly, the IMP level increased by up to 20-fold with respect to the value determined in control erythrocytes, when cells were challenged with the highest nonhaemolysing H 2 O 2 concentration (4 mm). Efflux of inosine, hypoxanthine, xanthine and uric acid towards the extracellular medium was observed. The metabolic imbalance of erythrocytes following oxidative stress was due to a dramatic and unexpected activation of AMPdeaminase (a twofold increase of activity with respect to controls) that was already evident at the lowest dose of H 2 O 2 used; this enzymatic activity increased with increasing H 2 O 2 in the medium, and reached its maximum at 4 mm H 2 O 2 -treated erythrocytes (10-fold higher activity than controls). Generation of malondialdehyde was strictly related to the dose of H 2 O 2 , being detectable at the lowest H 2 O 2 concentration and increasing without appreciable haemolysis up to 4 mm H 2 O 2 . Besides demonstrating a close relationship between lipid peroxidation and haemolysis, these data suggest that glycolytic enzymes are moderately affected by oxygen radical action and strongly indicate, in the change of AMP-deaminase activity, a highly sensitive enzymatic site responsible for a profound modification of erythrocyte energy metabolism during oxidative stress.Keywords: AMP-deaminase; energy metabolism; human erythrocytes; IMP; oxidative stress.Reactive oxygen species (ROS) are produced in living organisms from the partial reduction of molecular oxygen under physiological and pathological conditions. Because ROS has the capability of reacting nonspecifically with a vast number of compounds, all living organisms contain several enzymatic (superoxide dismutase, catalase, glutathione peroxidase) and nonenzymatic (a-tocopherol, ascorbic and uric acids, glutathione and other thiol protein groups) antioxidants with the specific purpose of protecting the functional and structural integrity of biologically fundamental macromolecules (nucleic acids, proteins, phospholipids). Imbalance between ROS production and antioxidant cell defences has been reported to occur in several physiopathological conditions of both animals and man: for example tissue ischemia and reperfusion Many in vitro and in vivo studies showed that several parameters of red blood cell function and integrity are ne...

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confidence: 99%

mentioning

confidence: 99%

Energy metabolism and lipid peroxidation of human erythrocytes as a function of increased oxidative stress

Tavazzi

Pierro

Amorini

et al. 2000

European Journal of Biochemistry

140

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“…Both lipophilic and cytoplasmic factors are thought to protect the erythrocyte membrane against lipid peroxidation. Foremost is ␣-tocopherol (4, 5), but ubiquinol-10 (6, 7), membrane protein sulfhydryls (8), and a GSH-dependent phospholipid hydroperoxidase (9,10) are also likely to contribute. Although ascorbic acid does not directly retard peroxidation of membrane lipids (11), it may do so indirectly by reducing the tocopheroxyl free radical in the lipid bilayer (12,13).…”

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Interaction of Ascorbate and α-Tocopherol in Resealed Human Erythrocyte Ghosts

May

Morrow

1996

Journal of Biological Chemistry

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A role for ascorbate-derived electrons in protection against oxidative damage to membrane lipids was investigated in resealed human erythrocyte ghosts. Incubation of resealed ghosts with the membrane-impermeant oxidant ferricyanide doubled the ghost membrane concentration of F 2 -isoprostanes, a sensitive marker of lipid peroxidation. Incorporation of ascorbate into ghosts during resealing largely prevented F 2 -isoprostane formation due to extravesicular ferricyanide. This protection was associated with a rapid transmembrane oxidation of intravesicular ascorbate by extravesicular ferricyanide. Transmembrane electron transfer, which was measured indirectly as ascorbate-dependent ferricyanide reduction, correlated with the content of ␣-tocopherol in the ghost membrane in several respects. First, ascorbate resealed within ghosts protected against ferricyanide-induced oxidation of endogenous ␣-tocopherol in the ghost membrane. Second, when exogenous ␣-tocopherol was incorporated into the ghost membrane during the resealing step, subsequent ferricyanide reduction was enhanced. Last, incubation of intact erythrocytes with soybean phospholipid liposomes, followed by resealed ghost preparation, caused a proportional decrease in both the membrane content of ␣-tocopherol and in ferricyanide reduction. Incorporation of exogenous ␣-tocopherol during resealing of ghosts prepared from liposome-treated cells completely restored the ferricyanide-reducing capacity of the ghosts. These results suggest that the transmembrane transfer of ascorbate-derived electrons in erythrocyte ghosts is dependent in part on ␣-tocopherol and that such transfer may help to protect the erythrocyte membrane against oxidant stress originating outside the cell.

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“…FOR cause oxidant-induced changes in the structure and function of erythrocyte membranes. Major changes reported by different researchers are lipid peroxidation, membrane protein-crosslinkings and oxidation of heme proteins resulting in their crosslinkings to skeletal proteins, i.e spectrin, actin, cytoplasmic component of band 3 (47)(48)(49)(50). Free oxygen radicals induced changes result in severe deteoriation of membrane structure and function causing increases in osmotic fragility ratios, accelerated cell aging and premature cell death ultimately.…”

Section: Discussionmentioning

confidence: 99%

The Oxidant Effects of Hyperbaric Oxygenation and Air Pollution in Erythrocyte Membranes (Hyperbaric Oxygenation in Air Pollution)

Etlik

Tomur

2006

ELECTRON J GEN MED

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Aim:The aim was to investigate the interactions of hyperbaric oxygenation (HBO) and sulphur dioxide (SO2) inhalation (at 10 ppm, one hour daily for 45 days) on lipoperoxidation and fragility of RBC membranes.Methods: Malonyldialdehyde (MDA) levels, osmotic fragility ratios and hematologic parameters of a total of 24 rats were compared in four different groups controls, HBO, SO2 and (HBO+SO2). HBO was applied at 3 Atm abs (303.9 kPa), 1 hour daily, for 45 days in a specifically designed exposure chamber.Results: MDA levels and osmotic fragility ratios were significantly higher in groups-B, C and D than in control group (p<.05 for all). Marked decrease in MDA levels and osmotic fragility ratios were observed in group-D (HBO+SO2) compared to groups-B and C (p<0.05). However, MDA levels and osmotic fragility ratios in group-D were also significantly higher than in group-A (p<0.05 for all).Conclusion: HBO-treatment or SO2 inhalation alone resulted in structural and functional oxidant damage, indicated by higher MDA levels and osmotic fragility ratios, but when they were applied together, decreases in oxidant damage in RBC membranes were observed indicating HBO may also be useful in the cities with a serious air pollution problem, at least it does not cause additional oxidant stress.

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The role of membrane protein sulfhydryl groups in hydrogen peroxide-mediated membrane damage in human erythrocytes

Cited by 64 publications

References 26 publications

Energy metabolism and lipid peroxidation of human erythrocytes as a function of increased oxidative stress

Energy metabolism and lipid peroxidation of human erythrocytes as a function of increased oxidative stress

Interaction of Ascorbate and α-Tocopherol in Resealed Human Erythrocyte Ghosts

The Oxidant Effects of Hyperbaric Oxygenation and Air Pollution in Erythrocyte Membranes (Hyperbaric Oxygenation in Air Pollution)

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