The role of Med19 in the proliferation and tumorigenesis of human hepatocellular carcinoma cells

Abstract

In addition, the ability of the Med19-RNAi-Lentivirus vector-infected Hep3B cells to form tumors after inoculation into nude mice was determined. Results: Recombinant lentiviral vectors expressing small interfering RNA (siRNA) against Med19 were constructed and were found to efficiently downregulate Med19 mRNA and protein levels in HepG2 and Hep3B cells. Furthermore, the inhibition of Med19 by RNAi dramatically reduced hepatocellular carcinoma cell proliferation, induced cell-cycle arrest in the G 0 /G 1 phase, and suppressed tumor formation. Conclusion: These results provide new evidence of an important role for Med19 in the development of hepatocellular carcinomas, suggesting that lentivirus-mediated RNAi to target Med19 is a potential tool for inhibiting cancer cell proliferation and tumorigenesis.

Suppression of MED19 expression by shRNA induces inhibition of cell proliferation and tumorigenesis in human prostate cancer cells

“…Furthermore, infection of lentivirus containing shRNA targeting MED19 resulted in lower colony formation ability and attenuated tumor formation and growth in PC-3 xenografts. This in vivo result provided new support for the role of MED19 in promoting neoplasia, which support the results of previous studies that reported an induction of tumorigenesis in hepatocellular carcinoma and lung cancer cells treated with MED19 siRNAs (14,15). Together, these findings justified the potential usefulness of MED19 as a prostate cancer biomarker.…”

Med19 promotes gastric cancer progression and cellular growth

“…Similar results have been obtained in the previous study. The suppression of Med19 results in an impaired cell proliferation in colonic, hepatic, lung, pancreatic, bladder, prostate, breast carcinoma, and osteosarcoma (Cui et al, 2011;Ji-Fu et al, 2011;Li et al, 2011aLi et al, , 2011bSun et al, 2011;Wang et al, 2011;Zhang et al, 2011;Zou et al, 2011). Collectively, the results presented in this paper, together with the previous reports, provide solid evidences for the involvement of Med19 in the development of malignancy.…”

Knockdown of Mediator Complex Subunit 19 Suppresses the Growth and Invasion of Prostate Cancer Cells

“…In lung cancer, MED19 could promote tumor proliferation, tumorigenesis, metastasis, and enhance chemo-sensitivity to cisplatin in non-small cell lung cancer cells [ 14 – 16 ]. The Med19 has been reported to promote tumor growth and/or metastasis in ovarian cancer, gastric cancer, pancreatic cancer, osteosarcoma, and liver cancer [ 5 – 7 , 17 – 18 ]. However, there are few reports about the roles of Med19 in PCa.…”