The Role of Mechanobiology in the Attachment of Tendon to Bone

Schwartz, Andrea G.; Thomopoulos, Stavros

doi:10.1007/978-1-4614-3317-0_11

Cited by 5 publications

(1 citation statement)

References 133 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the two collagens were also enriched in the enthesis proteome[ 4 ]. The list of top 10 genes also included MMP13 which is known to be involved in degradation of extracellular matrix[ 26 ] and may play a role in tissue remodeling processes to adapt to mechanical stresses[ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Biomarkers for tissue engineering of the tendon-bone interface

et al. 2018

View full text Add to dashboard Cite

The tendon-bone interface (enthesis) is a highly sophisticated biomaterial junction that allows stress transfer between mechanically dissimilar materials. The enthesis encounters very high mechanical demands and the regenerative capacity is very low resulting in high rupture recurrence rates after surgery. Tissue engineering offers the potential to recover the functional integrity of entheses. However, recent enthesis tissue engineering approaches have been limited by the lack of knowledge about the cells present at this interface. Here we investigated the cellular differentiation of enthesis cells and compared the cellular pattern of enthesis cells to tendon and cartilage cells in a next generation sequencing transcriptome study. We integrated the transcriptome data with proteome data of a previous study to identify biomarkers of enthesis cell differentiation. Transcriptomics detected 34468 transcripts in total in enthesis, tendon, and cartilage. Transcriptome comparisons revealed 3980 differentially regulated candidates for enthesis and tendon, 395 for enthesis and cartilage, and 946 for cartilage and tendon. An asymmetric distribution of enriched genes was observed in enthesis and cartilage transcriptome comparison suggesting that enthesis cells are more chondrocyte-like than tenocyte-like. Integrative analysis of transcriptome and proteome data identified ten enthesis biomarkers and six tendon biomarkers. The observed gene expression characteristics and differentiation markers shed light into the nature of the cells present at the enthesis. The presented markers will foster enthesis tissue engineering approaches by setting a bench-mark for differentiation of seeded cells towards a physiologically relevant phenotype.

show abstract

Section: Discussionmentioning

confidence: 99%

Biomarkers for tissue engineering of the tendon-bone interface

et al. 2018

View full text Add to dashboard Cite

show abstract

Hydroxyapatite nanocomposites for tendon repair

Xie

Jiang

Teusink

et al. 2016

Nanocomposites for Musculoskeletal Tissue Regeneration

View full text Add to dashboard Cite

The microstructure and micromechanics of the tendon–bone insertion

et al. 2017

View full text Add to dashboard Cite

The exceptional mechanical properties of the load-bearing connection of tendon to bone rely on an intricate interplay of its biomolecular composition, microstructure and micromechanics. Here we identify that the Achilles tendon-bone insertion is characterized by an interface region of ∼500 μm with a distinct fibre organization and biomolecular composition. Within this region, we identify a heterogeneous mechanical response by micromechanical testing coupled with multiscale confocal microscopy. This leads to localized strains that can be larger than the remotely applied strain. The subset of fibres that sustain the majority of loading in the interface area changes with the angle of force application. Proteomic analysis detects enrichment of 22 proteins in the interfacial region that are predominantly involved in cartilage and skeletal development as well as proteoglycan metabolism. The presented mechanisms mark a guideline for further biomimetic strategies to rationally design hard-soft interfaces.

show abstract

The Role of Mechanobiology in the Attachment of Tendon to Bone

Cited by 5 publications

References 133 publications

Biomarkers for tissue engineering of the tendon-bone interface

Biomarkers for tissue engineering of the tendon-bone interface

Hydroxyapatite nanocomposites for tendon repair

The microstructure and micromechanics of the tendon–bone insertion

Contact Info

Product

Resources

About