The Role of Mast Cells on Angiogenesis in Oral Squamous Cell Carcinoma

Kalra, Manpreet; Rao, Nirmala N; Nanda, Kanwardeep; Rehman, Farzan; Girish, KL; Tippu, Shoaib R; Arora, Asit

doi:10.4317/medoral.17395

Cited by 30 publications

(37 citation statements)

References 27 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, in this study, there was no statistically significant correlation between MCD with age, gender, and degree of tumor differentiation. In contrast, Kalra et al [18] showed that inter-comparison within the degree of differentiation with MCs count was decreased from well differentiated to poorly differentiated OSCC. The contribution of different etiological factors in the formation of OSCC in the two studies may have contributed to this difference.…”

Section: Discussionmentioning

confidence: 89%

Correlation of elevated mast cell and micro-vessel densities with lymph node metastasis in oral squamous cell carcinoma

Nakandala¹,

Suraweera²,

Jayasooriya³

2018

SDS

View full text Add to dashboard Cite

How to cite this article: Nakandala K, Suraweera A, Jayasooriya P. Correlation of elevated mast cell and micro-vessel densities with lymph node metastasis in oral squamous cell carcinoma. AbstractAim: Oral squamous cell carcinoma (OSCC) is a major health problem in Sri Lanka. Tumor-node-metastasis (TNM) staging system is the tool which is used to determine the extent of surgery in patients with OSCC. However, some patients may receive over-treatment with TNM staging. Mast cells can be found in the oral mucosa. Mast cells secrete different angiogenic factors stimulating angiogenesis which is important in metastasis of tumors. This study aimed to evaluate the mast cell density (MCD) and micro-vessel density (MVD) in OSCC and correlate it with nodal metastasis. Methods:The study sample consisted of one hundred OSCC specimens from patients who were treated surgically with both local excision of the tumor and neck dissections. The sections with OSCC present in achieved biopsies were evaluated for MCD and MVD using toluidine blue stain and a vascular marker (polyclonal rabbit anti-human von Willebrand factor) respectively. The correlation between MCD and MVD with nodal metastasis was statistically evaluated using point bi-serial correlation test.Results: There was a statistically significant correlation between MCD and nodal metastasis (P = 0.003) and MVD and nodal metastasis (P ≤ 0.000). These results indicate that MCD and MVD are significantly higher in OSCC with nodal metastasis. In addition, there was a weak positive correlation with MCD and MVD (r = 0.244) (Linear regression and Pearson correlation test). Higher statistically significant MCDs were observed in the buccal mucosal OSCC compared to OSCC of the tongue (n = 100, P = 0.023). There were no statistically significant differences when MCD and MVD were correlated with age, gender or degree of differentiation of the tumor. Conclusion:Significantly, high MCD and MVD at the advancing front of the OSCC might be used to identify tumors that present with nodal metastasis. With further studies, this finding could be applied to determine the extent of surgery required for patients with OSCC.

show abstract

Section: Discussionmentioning

confidence: 89%

Correlation of elevated mast cell and micro-vessel densities with lymph node metastasis in oral squamous cell carcinoma

Nakandala¹,

Suraweera²,

Jayasooriya³

2018

SDS

View full text Add to dashboard Cite

show abstract

“…First, unlike tumor cells, endothelial cells (ECs) are genomically stable, homogenous and could be ideal therapeutic targets that would not become resistant to anti-angiogenic therapy [5]. Second, turnover of tumor ECs is up to 50 times higher than that of endothelium in normal quiescent tissues [6]. Third, since anti-angiogenic therapy is directed at activated ECs, target would be easily accessible by systemic administration [6].…”

Section: Introductionmentioning

confidence: 99%

“…Second, turnover of tumor ECs is up to 50 times higher than that of endothelium in normal quiescent tissues [6]. Third, since anti-angiogenic therapy is directed at activated ECs, target would be easily accessible by systemic administration [6]. However, different mechanisms of acquired resistance to anti-angiogenic agents (e.g.…”

Section: Introductionmentioning

confidence: 99%

N-Hydroxy-6-(5-Nitro-Naphtalimide)-Hexanamide Inhibits Lysine Deacetylation, Mitigates Angiogenesis and Reduces Tumor Growth

Shankar

Sulka²,

Hubert

et al. 2015

J Cancer Sci

View full text Add to dashboard Cite

In this report, we present a novel histone deacetylase inhibitor (HDACi) (N-Hydroxy-6-(5-nitro-naphtalimide)-hexanamide: ES8) that efficiently inhibits angiogenesis in relevant ex vivo models (Human umbilical vein endothelial cells (HUVEC), 3D aortic ring assay) and in vivo (chick chorioallantoic membrane (CAM), Zebrafish). Transcriptomic profiling reveals a set of ES8 specific genes that are not affected by the prototypical HDACi suberoylanilide hydroxamic acid (SAHA). Finally, ES8 also reduced tumor growth in mouse models of small cell lung cancer. Availability of a novel compound not centered exclusively on inhibition of angiogenic factors and inducing a characteristic transcription profile may be of interest to overcome resistance to currently used chemotherapies.Citation: Shiva Shankar TV, Sulka B, Hubert P, Hubaux R, Delvenne P, et al. N-Hydroxy-6-(5-Nitro-Naphtalimide)-Hexanamide Inhibits Lysine Deacetylation, Mitigates Angiogenesis and Reduces Tumor Growth. J Cancer Sci. 2015;2(1): 11.J Cancer Sci 2(1): 11 (2015) Page -02 ISSN: 2377-9292 HDACi that potently inhibits angiogenesis and that induces a unique transcriptional profile not shared by two other reference inhibitors.

show abstract

“…Mast cells exert distinct non-immunological functions, being involved in tissue homeostasis, fibrosis and angiogenesis. The involvement of mast cells in angiogenesis has been strongly supported by clinico-pathological (3)(4)(5) and experimental studies (6,7) in different malignant and non-malignant diseases, but their role in the angiogenic process is still not well characterized. The involvement of mast cells in angiogenesis is supported by the ability of these cells to secrete growth factors and molecules that induce vascular hyperpermeability (8).…”

Section: Introductionmentioning

confidence: 99%

“…The involvement of mast cells in angiogenesis is supported by the ability of these cells to secrete growth factors and molecules that induce vascular hyperpermeability (8). Convincing data supporting a role for mast cells in tumor progression via formation of new blood vessels have accumulated (3,4). Because of the lack of data concerning the use of P1.HTR cells as an experimental tool for obtaining a mastocytoma tumor model, we proposed here to study the early and late effects of the P1.HTR cell line implanted on the chick embryo chorioallantoic membrane.…”

Section: Introductionmentioning

confidence: 99%

Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos

Avram¹,

Cimpean²,

Raica³

2013

Braz. J. Med. Biol. Res.

View full text Add to dashboard Cite

The P1.HTR cell line includes highly transfectable cells derived from P815 mastocytoma cells originating from mouse breast tissue. Despite its widespread use in immunogenic studies, no data are available about the behavior of P1.HTR cells in the chick embryo chorioallantoic membrane model. The objective of the present investigation was to study the effects of P1.HTR cells implanted on the chorioallantoic membrane of chick embryos. We inoculated P1.HTR cells into the previously prepared chick embryo chorioallantoic membrane and observed the early and late effects of these cells by stereomicroscopy, histochemistry and immunohistochemistry. A highly angiotropic and angiogenic effect occurred early after inoculation and a tumorigenic potential with the development of mastocytoma keeping well mast cells immunophenotype was detected later during the development. The P1.HTR mastocytoma cell line is a good tool for the development of the chick embryo chorioallantoic membrane mastocytoma model and also for other studies concerning the involvement of blood vessels. The chick embryo chorioallantoic membrane model of mastocytoma retains the mast cell immunophenotype under experimental conditions and could be used as an experimental tool for in vivo preliminary testing of antitumor and antivascular drugs.

show abstract

The Role of Mast Cells on Angiogenesis in Oral Squamous Cell Carcinoma

Cited by 30 publications

References 27 publications

Correlation of elevated mast cell and micro-vessel densities with lymph node metastasis in oral squamous cell carcinoma

Correlation of elevated mast cell and micro-vessel densities with lymph node metastasis in oral squamous cell carcinoma

N-Hydroxy-6-(5-Nitro-Naphtalimide)-Hexanamide Inhibits Lysine Deacetylation, Mitigates Angiogenesis and Reduces Tumor Growth

Behavior of the P1.HTR mastocytoma cell line implanted in the chorioallantoic membrane of chick embryos

Contact Info

Product

Resources

About