The role of manufacturers in reducing biofilms in dental chair waterlines

Coleman, David C.; O’Donnell, Mary; Shore, Anna C.; Swan, J.S.; Russell, R.J.

doi:10.1016/j.jdent.2007.05.003

Cited by 44 publications

(96 citation statements)

References 56 publications

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“…For the latter point, new agents are in development (1,49,50), but the role of DCU manufacturers in proposing DCUWs with reduced propensity to biofilm formation should also be required (51). From a more general point of view, we finally consider that the bacterial colonization of DCUWs can be used as a model to investigate the problem of waterborne colonization in health care settings.…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa and Achromobacter sp. Clonal Selection Leads to Successive Waves of Contamination of Water in Dental Care Units

Abdouchakour

Dupont

Grau

et al. 2015

Appl Environ Microbiol

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Dental care unit waterlines (DCUWs) consist of complex networks of thin tubes that facilitate the formation of microbial biofilms. Due to the predilection toward a wet environment, strong adhesion, biofilm formation, and resistance to biocides, Pseudomonas aeruginosa, a major human opportunistic pathogen, is adapted to DCUW colonization. Other nonfermentative Gramnegative bacilli, such as members of the genus Achromobacter, are emerging pathogens found in water networks. We reported the 6.5-year dynamics of bacterial contamination of waterlines in a dental health care center with 61 dental care units (DCUs) connected to the same water supply system. The conditions allowed the selection and the emergence of clones of Achromobacter sp. and P. aeruginosa characterized by multilocus sequence typing, multiplex repetitive elements-based PCR, and restriction fragment length polymorphism in pulsed-field gel electrophoresis, biofilm formation, and antimicrobial susceptibility. One clone of P. aeruginosa and 2 clones of Achromobacter sp. colonized successively all of the DCUWs: the last colonization by P. aeruginosa ST309 led to the closing of the dental care center. Successive dominance of species and clones was linked to biocide treatments. Achromobacter strains were weak biofilm producers compared to P. aeruginosa ST309, but the coculture of P. aeruginosa and Achromobacter enhanced P. aeruginosa ST309 biofilm formation. Intraclonal genomic microevolution was observed in the isolates of P. aeruginosa ST309 collected chronologically and in Achromobacter sp. clone A. The contamination control was achieved by a complete reorganization of the dental health care center by removing the connecting tubes between DCUs. D ental instruments on dental care units (DCUs), such as ultrasonic scalers, air scalers, and high-speed turbine dental handpieces, are cooled by DCU-supplied water, which also irrigates and cools the tooth surface during dental treatment (1). Water is also supplied to the DCU cup filler outlet used by patients for oral rinsing and to the bowl rinse outlet rinsing the DCU spittoon. Therefore, DCU waterlines (DCUWs) consist of a complex network formed by meters of narrow-bore plastic tubing of mostly 2-to 3-mm internal diameter. In narrow-bore tubes, a thin immobile layer of fluid, called the hydrodynamic boundary layer, exists at the interface of the lumen wall and the moving water (2). This hydrodynamic phenomenon is associated with water stagnation when the DCU is not used, and heating of water to at least 20°C enhances the development of microbial biofilm in the tubes (3).The DCUWs appear to be a technological niche in which complex interactions occur among members of the microbial community (4-6). Regarding bacteria, DCUWs have been shown to promote the formation of biofilm, predominantly seeded from environmental bacteria in the water supply (4-6). More rarely, human bacteria such as staphylococci or oral bacteria have been detected, indicating a retraction of oral fluids from the surgical devices to the wate...

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Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa and Achromobacter sp. Clonal Selection Leads to Successive Waves of Contamination of Water in Dental Care Units

Abdouchakour

Dupont

Grau

et al. 2015

Appl Environ Microbiol

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“…[1][2][3][4] DCUs use water to irrigate the oral cavity and tooth surfaces during dental procedures via three-in-one air/water syringes and provide cooling to the dynamic instruments (e.g., turbine and conventional handpieces and ultrasonic scalers) as heat generated during instrument operation can be detrimental to teeth. [5][6][7] DCUs also provide water for oral rinsing by patients (water supplied via the cup filler outlet) and to rinse the DCU spittoon, or cuspidor bowl, after oral rinsing (water supplied via the bowl rinse outlet).…”

Section: Introductionmentioning

confidence: 99%

A centralised, automated dental hospital water quality and biofilm management system using neutral Ecasol™ maintains dental unit waterline output at better than potable quality: A 2-year longitudinal study

O’Donnell

Boyle

Swan

et al. 2009

Journal of Dentistry

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“…In the group A, the incoming dental unit water's one, most jobs regarded the microbial contamination of dental unit water (1,3,4,12), the dental unit bacterial biofilm (2,6,8,9,11) and the many disinfection protocols (5,7,13,16) (Table 3). Group B, that collect jobs about dental unit wastewater, is really scant, in fact there are only 8 works and all of them analyzed the problem of dental amalgam in wastewater (Table 4).…”

Section: Resultsmentioning

confidence: 99%

“…The dental unit is equipped with an elaborate loom of interconnected narrow-bore (2-3 mm internal diameter) flexible plastic tubing called dental unit waterlines that supply water to all of the dental unit instruments, cup-filler and bowlrinse water outles (8,9).…”

Section: Incoming Dental Unit Watermentioning

confidence: 99%

Dental Unit Wastewater, a Current Environmental Problem: A Sistematic Review

Arcuri

et al. 2017

ORL

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The role of manufacturers in reducing biofilms in dental chair waterlines

Cited by 44 publications

References 56 publications

Pseudomonas aeruginosa and Achromobacter sp. Clonal Selection Leads to Successive Waves of Contamination of Water in Dental Care Units

Pseudomonas aeruginosa and Achromobacter sp. Clonal Selection Leads to Successive Waves of Contamination of Water in Dental Care Units

A centralised, automated dental hospital water quality and biofilm management system using neutral Ecasol™ maintains dental unit waterline output at better than potable quality: A 2-year longitudinal study

Dental Unit Wastewater, a Current Environmental Problem: A Sistematic Review

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