The Role of Lysosomes in Molluscan Inflammation1

Cheng, Thomas C.

doi:10.1093/icb/23.1.129

Cited by 132 publications

(55 citation statements)

References 47 publications

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“…According to Cheng (1983) and Renwrantz (1990), the phagocytic process can be subdivided into 4 steps: chemotaxis, recognition, internalization and intracellular degradation. Phagocytosis is, therefore, fundamentally dependent on membrane communication, for chemotaxis, fluidity of movement and internalization, as well as maintenance of internal ionic balance for the biochemical degradation.…”

Section: Discussionmentioning

confidence: 99%

Hemocyte functions and bacterial clearance affected in vivo by TBT and DBT in the blue mussel Mytilus edulis

St-Jean¹,

Pelletier²,

Courtenay³

2002

Mar. Ecol. Prog. Ser.

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Section: Discussionmentioning

confidence: 99%

Hemocyte functions and bacterial clearance affected in vivo by TBT and DBT in the blue mussel Mytilus edulis

St-Jean¹,

Pelletier²,

Courtenay³

2002

Mar. Ecol. Prog. Ser.

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“…In bivalves, haemocyte lysosomes are known to be involved in the intracellular degradation of digestible material and the release of hydrolytic enzymes during phagocytosis in response to infection (reviewed in Cheng 1983). Since their membranes are susceptible to being destabilised by different stressors, this feature has been frequently used as a biomarker to monitor pollution and animal health (reviewed in Moore et al 2004Moore et al , 2006.…”

Section: Abstract: Mya Arenaria · Vibrio Splendidus · Haemocyte Subpmentioning

confidence: 99%

Changes induced by two strains of Vibrio splendidus in haemocyte subpopulations of Mya arenaria, detected by flow cytometry with LysoTracker

Mateo¹,

Spurmanis²,

Siah³

et al. 2009

Dis. Aquat. Org.

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“…Lytic enzymes play a key role in these processes. They are produced in the granulocytes and released into phagosomes (Cheng & Cali 1974, Cheng & Rodrick 1975, Cheng 1983, Yoshino 1988, Pipe 1990, Pipe et al 1995, Cajaraville et al 1996, Carballal et al 1997c. Lysosomal hydrolases are compartmentalized within electron-dense specific granules (Yoshino & Cheng 1976, Rodrick & Ulrich 1984, Pipe 1990.…”

Section: Introductionmentioning

confidence: 99%

Morphological and cytoenzymatic characterization of haemocytes of the venus clam Chamelea gallina

Pampanin

Marin²,

Ballarin³

2002

Dis. Aquat. Org.

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A morphological and enzymatic characterization of Chamelea gallina haemocytes was carried out as a prerequisite for further studies on venus clam immunobiology. Two main types of circulating haemocytes were identified (1) hyalinocytes (79.2%), agranular cells with a central nucleus surrounded by a little cytoplasm, and (2) granulocytes (16.5%), smaller granular cells with smaller nuclei. Small cells with a strongly basophilic nucleus and a thin layer of peripheral cytoplasm, probably undifferentiated blast cells (4.3%), were also observed. Both granulocytes and hyalinocytes can assume a spreading or round morphology. The enzymatic activities of haemocytes were also investigated. Some of the granulocytes and hyalinocytes were positive for hydrolytic enzymes, suggesting a role for these cells in phagocytosis; no oxidative enzymes were detected in C. gallina haemocytes. Granulocytes and hyalinocytes can easily adhere to the substratum and exhibit a low phagocytosis activity towards foreign particles (6.3%), whereas the fraction of cells containing ingested material significantly increased after pre-incubation of test particles with cell-free haemolymph, which suggests the presence of opsonin(s) in the haemolymph. KEY WORDS: Haemocytes · Chamelea gallina · Cytoenzymology · Phagocytosis Resale or republication not permitted without written consent of the publisherDis Aquat Org 49: [227][228][229][230][231][232][233][234] 2002 (Cheney 1971, Foley & Cheng 1974, Moore & Eble 1977, Huffman & Tripp 1982. Since no data are available concerning Chamelea gallina haemocytes and their role in immunity, we carried out a morpho-functional characterization of C. gallina haemocytes as a prerequisite for further studies on the immunobiology of the venus clam in order to define a series of immuno-biomarkers to be used in the evaluation of stress conditions for this species.Our results indicate the presence of 2 main haemocyte types, namely granulocytes and hyalinocytes, both exhibiting lysosomal enzymes and phagocytosis activity. MATERIALS AND METHODSClams (shell length 20 to 30 mm) were collected with a hydraulic dredge at Venice Lido, on the west coast of the Northern Adriatic Sea, in front of the Venice lagoon. They were kept in the laboratory for 2 to 3 d before experiments in 70 l tanks filled with aerated seawater that was renewed every other day (salinity 35 ± 1 ‰; temperature 17 ± 1°C) and fed with microalgae (Isochrysis galbana and Phaeodactylum tricornutum).Heamolymph collection. Haemolymph was collected from the anterior adductor muscle of clams with a syringe containing 10 mM of L-cysteine in filtered seawater (FSW), pH 7.5, to a final dilution of 1:1 haemolymph/L-cysteine. Haemolymph was also collected in the absence of L-cysteine and centrifuged at 780 × g for 10 min to pellet cells and obtain cell-free haemolymph (CFH).Haemocyte cultures. Haemolymph was centrifuged at 780 × g for 10 min and the pellet was resuspended in FSW to achieve a final concentration of 10 6 cells ml -1 . Sixty µl of haemocyte susp...

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The Role of Lysosomes in Molluscan Inflammation1

Cited by 132 publications

References 47 publications

Hemocyte functions and bacterial clearance affected in vivo by TBT and DBT in the blue mussel Mytilus edulis

Hemocyte functions and bacterial clearance affected in vivo by TBT and DBT in the blue mussel Mytilus edulis

Changes induced by two strains of Vibrio splendidus in haemocyte subpopulations of Mya arenaria, detected by flow cytometry with LysoTracker

Morphological and cytoenzymatic characterization of haemocytes of the venus clam Chamelea gallina

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