The role of long noncoding RNAs in cancer metastasis

Parsons, Christine; Tayoun, Alexis M.; Benado, Benjamin D.; Ragusa, Gabriel; Dorvil, Rose F.; Rourke, Elizabeth; Connor, Kaitlyn O’; Reed, Irene G.; Alexander, Angela; Willetts, Lian; Habibian, Maryam; Adams, Brian D.

doi:10.20517/2394-4722.2018.11

Cited by 10 publications

(3 citation statements)

References 190 publications

(226 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It seems that cancerous cells are capable of signalling the cells of distant tissues, resulting in the remodelling of those tissues to better support metastatic tumour growth. The signals conveyed by cancerous cells seem to be in the form of ncRNA [9, 45–47].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Specific trophoblast transcripts transferred by extracellular vesicles affect gene expression in endometrial epithelial cells and may have a role in embryo-maternal crosstalk

et al. 2019

View full text Add to dashboard Cite

BackgroundSuccessful establishment of pregnancy hinges on appropriate communication between the embryo and the uterus prior to implantation, but the nature of this communication remains poorly understood. Here, we tested the hypothesis that the endometrium is receptive to embryo-derived signals in the form of RNA.MethodsWe have utilized a non-contact co culture system to simulate the conditions of pre implantation environment of the uterus. We bioorthogonally tagged embryonic RNA and tracked the transferred transcripts to endometrium. Transferred transcripts were separated from endometrial transcripts and sequenced. Changes in endometrial transcripts were quantified using quantitative PCR.ResultsWe show that three specific transcripts are transferred to endometrial cells. We subsequently demonstrate a role of extracellular vesicles (EVs) in this process, as EVs obtained from cultured trophoblast spheroids incubated with endometrial cells induced down-regulation of all the three identified transcripts in endometrial cells.Finally, we show that EVs/nanoparticles captured from conditioned culture media of viable embryos as opposed to degenerating embryos induce ZNF81 down-regulation in endometrial cells, hinting at the functional importance of this intercellular communication.ConclusionUltimately, our findings demonstrate the existence of an RNA-based communication which may be of critical importance for the establishment of pregnancy.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Recent investigations have pointed to the exchange of different forms of ncRNA as a major component of cell-to-cell communication [7–9]. These exchanges seems to be partly mediated through extracellular vesicles (EV) [10].…”

Section: Introductionmentioning

confidence: 99%

Specific trophoblast transcripts transferred by extracellular vesicles affect gene expression in endometrial epithelial cells and may have a role in embryo-maternal crosstalk

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Likewise, ANRIL is highly expressed in cancers including non-small cell lung cancer and cervical cancer, its depletion inhibits cell proliferation [186,187]. In addition to these antisense transcripts, there are other antisense transcripts involved in various cancers [188,189,190,191,192,193,194,195,196]; thus, it could serve as potential biomarkers and/or therapeutic targets for cancer therapy. Furthermore, a comprehensive dataset has been generated for a positive correlation of the differential expressions of sense–antisense transcripts with cancer [197,198].…”

Section: Antisense Transcription In Cancermentioning

confidence: 99%

Mechanisms of Antisense Transcription Initiation with Implications in Gene Expression, Genomic Integrity and Disease Pathogenesis

Barman

Reddy

Bhaumik

2019

ncRNA

View full text Add to dashboard Cite

Non-coding antisense transcripts arise from the strand opposite the sense strand. Over 70% of the human genome generates non-coding antisense transcripts while less than 2% of the genome codes for proteins. Antisense transcripts and/or the act of antisense transcription regulate gene expression and genome integrity by interfering with sense transcription and modulating histone modifications or DNA methylation. Hence, they have significant pathological and physiological relevance. Indeed, antisense transcripts were found to be associated with various diseases including cancer, diabetes, cardiac and neurodegenerative disorders, and, thus, have promising potentials for prognostic and diagnostic markers and therapeutic development. However, it is not clearly understood how antisense transcription is initiated and epigenetically regulated. Such knowledge would provide new insights into the regulation of antisense transcription, and hence disease pathogenesis with therapeutic development. The recent studies on antisense transcription initiation and its epigenetic regulation, which are limited, are discussed here. Furthermore, we concisely describe how antisense transcription/transcripts regulate gene expression and genome integrity with implications in disease pathogenesis and therapeutic development.

show abstract