The role of leukotriene C4 in biphasic release of histamine from mouse mastocytoma cell line (P-815)

Teshigawara, Hiroshi; Moriya, Yoshihiko

doi:10.1016/0306-3623(94)90087-6

Cited by 1 publication

(1 citation statement)

References 24 publications

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“…P‐815 cells (2–4×10 7 cells) were harvested by centrifuging 50 ml of the suspension and washing the cells three times with phosphate‐buffered saline (PBS). Mastocytoma cells (4–6×10 6 cells) in 1 ml of PBS were incubated for 20 min after treatment with compound 48/80, beta‐toxin or substance P (Teshigawara & Moriya, 1994). The release of histamine was measured by the o‐phthalaldehyde spectrometric procedure as described previously (Sakurai & Fujii, 1987).…”

Section: Methodsmentioning

confidence: 99%

Involvement of tachykinin receptors in Clostridium perfringens beta‐toxin‐induced plasma extravasation

Nagahama

Morimitsu

Kihara

et al. 2003

British J Pharmacology

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1 Clostridium perfringens beta-toxin causes dermonecrosis and oedema in the dorsal skin of animals. In the present study, we investigated the mechanisms of oedema induced by the toxin. 2 The toxin induced plasma extravasation in the dorsal skin of Balb/c mice. 3 The extravasation was signi®cantly inhibited by diphenhydramine, a histamine 1 receptor antagonist. However, the toxin did not cause the release of histamine from mouse mastocytoma cells. ]-SP and spantide, inhibited the toxin-induced leakage in a dose-dependent manner. Furthermore, the non-peptide tachykinin NK 1 receptor antagonist, SR140333, markedly inhibited the toxin-induced leakage. 5 The leakage induced by the toxin was markedly reduced in capsaicin-pretreated mouse skin but the leakage was not aected by systemic pretreatment with a calcitonin gene-related peptide receptor antagonist ]-bradykinin), but was not aected by the selective L-type Ca 2+ channel blocker, verapamil, the P-type Ca 2+ channel blocker, o-agatoxin IVA, tetrodotoxin (TTX), the TTX-resistant Na + channel blocker, carbamazepine, or the sensory nerve conduction blocker, lignocaine. 7 These results suggest that plasma extravasation induced by beta-toxin in mouse skin is mediated via a mechanism involving tachykinin NK 1 receptors.

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Section: Methodsmentioning

confidence: 99%