The role of l-cysteine/Hydrogen sulfide pathway on β3-Adrenoceptor- induced relaxation in mouse gastric fundus

Adıbelli, Elif Özveren; Aydinoglu, Fatma; Ogulener, Nuran

doi:10.1016/j.niox.2021.12.004

Cited by 2 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the activation of β 3 AR on the urothelium leads to an increase in CSE-derived H 2 S, which contributes to the relaxant effect. These findings converge with other pieces of evidence present in the relevant literature reporting that β 3 AR activation in other districts, such as mouse stomach and human corpus cavernosum, involves H 2 S signaling [ 33 , 53 ]. It is feasible to speculate that the contribution of CBS and/or CSE in β 3 AR activation may depend upon the district/organ.…”

Section: Discussionsupporting

confidence: 90%

β3 Relaxant Effect in Human Bladder Involves Cystathionine γ-Lyase-Derived Urothelial Hydrogen Sulfide

et al. 2022

View full text Add to dashboard Cite

It is now well established that the urothelium does not act as a passive barrier but contributes to bladder homeostasis by releasing several signaling molecules in response to physiological and chemical stimuli. Here, we investigated the potential contribution of the hydrogen sulfide (H2S) pathway in regulating human urothelium function in β3 adrenoceptor-mediated relaxation. The relaxant effect of BRL 37344 (0.1–300 µM), a selective β3 adrenoceptor agonist, was evaluated in isolated human bladder strips in the presence or absence of the urothelium. The relaxant effect of BRL 37344 was significantly reduced by urothelium removal. The inhibition of cystathionine-γ-lyase (CSE), but not cystathionine-β-synthase (CBS), significantly reduced the BRL 37344 relaxing effect to the same extent as that given by urothelium removal, suggesting a role for CSE-derived H2S. β3 adrenoceptor stimulation in the human urothelium or in T24 urothelial cells markedly increased H2S and cAMP levels that were reverted by a blockade of CSE and β3 adrenoceptor antagonism. These findings demonstrate a key role for urothelium CSE-derived H2S in the β3 effect on the human bladder through the modulation of cAMP levels. Therefore, the study establishes the relevance of urothelial β3 adrenoceptors in the regulation of bladder tone, supporting the use of β3 agonists in patients affected by an overactive bladder.

show abstract

Section: Discussionsupporting

confidence: 90%