The Role of Kinesin Neck Linker and Neck in Velocity Regulation

The Effect of Monastrol on the Processive Motility of a Dimeric Kinesin-5 Head/Kinesin-1 Stalk Chimera

“…9 The processive movement of single Eg5Kin dimers proves that the Eg5 motor domains are intrinsically capable of extended processive motility. The increased run length of Eg5Kin, as compared to HsEg5-513, is consistent with two studies recently published by Kalchishkova and Bohm 23 and 24 The small globular tail domain of Eg5 contains the BimC box. (b) Details of the junction between the Eg5 motor domain and the DmKHC neck coiled coil: The numbering refers to the amino acid numbering in the respective wild-type motor sequences as indicated in gray.…”

“…Expression and purification were performed as previously described in Coy et al (for a more detailed description of materials and methods, see Supplementary Information). 25 Muthukrishnan et al 29 and with a biochemical study by Hackney et al 30 In the first study, in which a human kinesin-5 motor domain, including its neck linker, was fused to a human neuronal kinesin-1 (construct E-K(neck)), 23 the authors observed an increase in speed as compared to a presumably dimeric truncation of human kinesin-5. Processivity was not assessed.…”

The Structural Basis of Force Generation by the Mitotic Motor Kinesin-5

“…the N terminus and NL of individual MDs have coevolved and must be paired to generate wild-type motility) (62). CNB formation also has a significant regulatory effect on K5 nucleotide affinity and motor ATPase (32,62), further highlighting the numerous allosteric effects transmitted across the K5 MD (59).…”

α-Synuclein Oligomers Impair Neuronal Microtubule-Kinesin Interplay

“…Kinesins were expressed in E. coli as fusion proteins with a C-terminal chitinbinding intein tag and purified by binding to chitin beads followed by removal of the intein tag by 50 mM DTT-induced specific self-cleavage (25). The kinesins were stored in motility buffer (50 mM imidazole, 0.5 mM MgCl 2 , 0.5 mM EGTA, 0.5 mM DTT, pH 6.8) supplemented with 150 mM NaCl and 1 M glycerol at Ϫ80°C.…”