The role of key genes in astaxanthin biosynthesis in Phaffia rhodozyma by transcript level and gene knockout

Li, Zhipeng; Chen, Lina; Yang, Haoyi; Li, Tianli; Du, Xiping; He, Ning; Jiang, Zhen; Li, Lijun; Ni, Hui

doi:10.1016/j.procbio.2021.12.029

Cited by 4 publications

(5 citation statements)

References 36 publications

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“…However, it could still counteract the inhibitory effect of higher temperature and effectively transfer phytoene to lycopene for astaxanthin synthesis 23 . Moreover, study found that the total fatty acid content of mutants at 25°C was lower than that of wild type, resulting in the accumulation of acetyl‐CoA, NADPH, and ATP, which were transferred to astaxanthin flux 24 . Researchers also found that astaxanthin content was negatively correlated with fatty acid content 22 .…”

Section: Resultsmentioning

confidence: 99%

“…23 Moreover, study found that the total fatty acid content of mutants at 25 • C was lower than that of wild type, resulting in the accumulation of acetyl-CoA, NADPH, and ATP, which were transferred to astaxanthin flux. 24 Researchers also found that astaxanthin content was negatively correlated with fatty acid content. 22 These phenomena might explain the higher carotenoid production of mutants than that of original strains grown at 25 • C.…”

Section: Carotenoid Production and Hereditary Stability Of Mutant Str...mentioning

confidence: 99%

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Enhancement of astaxanthin production from food waste by Phaffia rhodozyma screened by flow cytometry and feed application potential

Lai

Liu

et al. 2023

Biotech and App Biochem

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Astaxanthin is widely used in food, aquaculture, cosmetics, and pharmaceuticals due to its strong antioxidant activity and coloring ability, but its production from Phaffia rhodozyma remains the main challenge due to the high fermentation cost and low content of carotenoid. In this study, the production of carotenoids from food waste (FW) by a P. rhodozyma mutant was investigated. P. rhodozyma mutant screened by UV mutagenesis and flow cytometry could stably produce high carotenoids at 25°C, with carotenoid production (32.9 mg/L) and content (6.7 mg/g), respectively, increasing by 31.6% and 32.3% compared with 25 mg/L and 5.1 mg/g of wild strain. Interestingly, the carotenoid production reached 192.6 mg/L by feeding wet FW, which was 21% higher than batch culture. The 373 g vacuum freeze‐dried products were obtained from the fermentation of 1 kg FW by P. rhodozyma, which contained 784 mg carotenoids and 111 mg astaxanthin. The protein, total amino acids, and essential amino acids content of the fermentation products were 36.6%, 40.5%, and 18.2% (w/w), respectively, and lysine‐added fermentation products had the potential of high‐quality protein feed source. This study provides insights for the high‐throughput screening of mutants, astaxanthin production, and the development of the feed potential of FW.

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Section: Resultsmentioning

confidence: 99%

Section: Carotenoid Production and Hereditary Stability Of Mutant Str...mentioning

confidence: 99%

Enhancement of astaxanthin production from food waste by Phaffia rhodozyma screened by flow cytometry and feed application potential

Lai

Liu

et al. 2023

Biotech and App Biochem

View full text Add to dashboard Cite

show abstract

“…Li et al. ( 2022 ) adjusted the metabolic node by directly adding additives, and increased astaxanthin production by strengthening the carotenoid synthesis pathway and weakening the competitive pathway. Luna‐Flores et al.…”

Section: Resultsmentioning

confidence: 99%

“…The strain JMU-MVP14 of P. rhodozyma (Li et al., 2022 ; Xiao et al., 2015 ; Yang et al., 2023 ) was obtained from the strain bank of the Fujian Key Laboratory of Food Microbiology and Enzyme Engineering. Astaxanthin standard products were purchased from Yuanye Biotechnology Co., Ltd (SHH, CHN).…”

Section: Methodsmentioning

confidence: 99%

Transcriptomics analysis and fed-batch regulation of high astaxanthin-producing Phaffia rhodozyma/Xanthophyllomyces dendrorhous obtained through adaptive laboratory evolution

Yang

Hong-yuan

et al. 2023

Journal of Industrial Microbiology and Biotechnology

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Astaxanthin has high utilization value in functional food because of its strong antioxidant capacity. However, the astaxanthin content of Phaffia rhodozyma is relatively low. Adaptive laboratory evolution is an excellent method to obtain high-yield strains. TiO2 is a good inducer of oxidative stress. In this study, different concentrations of TiO2 were used to domesticate P. rhodozyma. Results showed that the optimal astaxanthin-producing P. rhodozyma JMU-ALE105 was obtained under domestication with 1000 mg/L TiO2 concentration on the 105th day. After fermentation, the astaxanthin content reached 6.50 mg/g, which was 41.61% higher than that of the original strain. The ALE105 strain was fermented by batch and fed-batch, and the astaxanthin content reached 6.81 mg/g. Transcriptomics analysis showed that astaxanthin synthesis pathway, fatty acid, pyruvate and nitrogen metabolism pathway of ALE105 strain were significantly up-regulated. Based on the nitrogen metabolism pathway, the nitrogen source was adjusted by ammonium sulphate fed-batch fermentation, which increased the astaxanthin content reached 8.36 mg/g. This study provides technical basis and theoretical research for promoting industrialization of astaxanthin production of P. rhodozyma.

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“…It can also be converted into astaxanthin by CrtR and CrtO. At the same time, CrtS, the protein translated after astaxanthin synthetase), can continuously catalyze the 4-ketolation and 3-hydroxylation of β-carotene, to synthesize astaxanthin [24]. However, according to Li et al's study, in three different P.rhodozyma strains, the CrtS gene was poorly correlated with astaxanthin synthesis [8,25].…”

Section: Transcriptome and Metabolome Analysis And Discussion Of The ...mentioning

confidence: 99%

Enhanced Carotenoid Production in Chlamydomonas reinhardtii by Overexpression of Endogenousand Exogenous Beta-Carotene Ketolase (BKT) Genes

Chen

Liang

et al. 2023

IJMS

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Chlamydomonas reinhardtii is a unicellular green alga that can grow heterotrophically by using acetate as a carbon source. Carotenoids are natural pigments with biological activity and color, which have functions such as antioxidant, anti-inflammatory, vision protection, etc., and have high commercial value and prospects. We transformed Chlamydomonas reinhardtii with the BKT genes from Phaffia rhodozyma (PrBKT) and Chlamydomonas reinhardtii (CrBKT) via plasmid vector, and screened out the stable transformed algal strains C18 and P1. Under the condition that the cell density of growth was not affected, the total carotenoid content of C18 and P1 was 2.13-fold and 2.20-fold higher than that of the WT, respectively. CrBKT increased the levels of β-carotene and astaxanthin by 1.84-fold and 1.21-fold, respectively, while PrBKT increased them by 1.11-fold and 1.27-fold, respectively. Transcriptome and metabolome analysis of C18 and P1 showed that the overexpression of CrBKT only up-regulated the transcription level of BKT and LCYE (the gene of lycopene e-cyclase). However, in P1, overexpression of PrBKT also led to the up-regulation of ZDS (the gene of ζ-carotene desaturase) and CHYB (the gene of β-carotene hydroxylase). Metabolome results showed that the relative content of canthaxanthin, an intermediate metabolite of astaxanthin synthesis in C18 and P1, decreased. The overall results indicate that there is a structural difference between CrBKT and PrBKT, and overexpression of PrBKT in Chlamydomonas reinhardtii seems to cause more genes in carotenoid pathway metabolism to be up-regulated.

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The role of key genes in astaxanthin biosynthesis in Phaffia rhodozyma by transcript level and gene knockout

Cited by 4 publications

References 36 publications

Enhancement of astaxanthin production from food waste by Phaffia rhodozyma screened by flow cytometry and feed application potential

Enhancement of astaxanthin production from food waste by Phaffia rhodozyma screened by flow cytometry and feed application potential

Transcriptomics analysis and fed-batch regulation of high astaxanthin-producing Phaffia rhodozyma/Xanthophyllomyces dendrorhous obtained through adaptive laboratory evolution

Enhanced Carotenoid Production in Chlamydomonas reinhardtii by Overexpression of Endogenousand Exogenous Beta-Carotene Ketolase (BKT) Genes

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