The role of jak2a in zebrafish hematopoiesis

Abstract

Janus kinase 2 (Jak2) transduces signals from hematopoietic cytokines, and a gainof-function mutation (Jak2 617V>F ) is associated with myeloproliferative diseases, particularly polycythemia vera. In this study, we examined the role of jak2a in zebrafish embryos in knock-down and overexpression studies using morpholinos (

The role of survivin2 in primitive hematopoiesis during zebrafish development

“…For instance, similar to the sur1 knockdown, 13 the development of eye and brain in Sur2 MO embryos at 24 and 48 h.p.f. was slightly delayed.…”

“…Procedures for microinjection, whole mount in situ hybridization (ISH), O-dianisidine staining, terminal transferase dUTP nick-end labeling (TUNEL) and caspase-3 activity assays have been described previously. [11][12][13] Amino acids sequences alignment and construction of phylogenetic tree…”

“…A random sequence Comparison of gene expression between control and the Sur2 MO embryos in each case was based on DC T as described previously. 13 Statistical analysis was evaluated by Student's paired t-test. The data as shown represented average results of four experiments using more than 800 embryos.…”

“…embryos were reverse transcribed from RNA and quantitative reverse transcription (RT)-PCR (Q-PCR) for various hematopoietic genes was performed using the SYBR green PCR master mix (Applied Biosystems, Foster City, CA, USA). Expression level was presented as fold-change calculated using the comparative C T method as described 12,13 with b-actin as the internal reference. Primer sequences for Q-PCR were shown in Table 1.…”

Comparison of mutated ABL1 and JAK2 as oncogenes and drug targets in myeloproliferative disorders

“…44 In the zebrafish model, JAK2 is involved in primitive hematopoiesis whereas deletion of JAK2 alleles in mice result in embryonic lethality probably due to fatal anemia. 45,46 The JAK2 gene is located on chromosome band 9p24 and its activity is required for signaling by several growth factor receptors that lack intrinsic TK activity, such as the erythropoietin receptor (EpoR), the thrombopoietin receptor (Mpl) and the granulocyte colony-stimulating factor receptor (G-CSFR). Binding of ligand leads to receptor dimerization with subsequent conformational changes of the bound JAK2 enzymes on the inner cellular membrane.…”