The Role of inab in Axon Morphology of an Identified Zebrafish Motoneuron

Ryswyk, Liesl Van; Simonson, Levi; Eisen, Judith S

doi:10.1371/journal.pone.0088631

Cited by 7 publications

(4 citation statements)

References 54 publications

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“…However, there were some differences between the expression patterns of inab and mammalian α‐internexin; the former was only found in ganglion cells of the retina (Leake et al, ), whereas mammalian α‐internexin was found in retinal ganglion cells as well as in retinal interneurons, such as amacrine cells (Chien and Liem, ). In contrast to inab , the investigation of the other homolog of mammalian α‐internexin, inaa , was limited: it was only reported to be expressed in the diencephalon of the zebrafish embryo at 24 hpf (Van Ryswyk et al, ). Moreover, the localization of inaa in the developing and adult zebrafish retina remains unknown.…”

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confidence: 99%

Developmental pattern of the neuronal intermediate filament inaa in the zebrafish retina

Liao

Peng

Kan

et al. 2016

J of Comparative Neurology

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α-Internexin is a member of the neuronal intermediate filament (nIF) protein family, which also includes peripherin and neurofilament (NF) triplet proteins. Previous studies found that expression of α-internexin precedes that of the NF triplet proteins in mammals and suggested that α-internexin plays a key role in the neuronal cytoskeleton network during development. In this study, we aimed to analyze the expression patterns and function of internexin neuronal intermediate filament protein-alpha a (inaa), the encoding gene of which is a homolog of the mammalian α-internexin, during retinal development in zebrafish. Via in vitro and in vivo studies, we demonstrated that zebrafish inaa is an α-internexin homolog that shares characteristics with nIFs. An immunohistochemical analysis of zebrafish revealed that inaa was distributed dynamically in the developing retina. It was widely localized in retinal neuroepithelial cells at 1 day postfertilization (dpf), and was mainly found in the ganglion cell layer (GCL) and inner part of the inner nuclear layer (INL) from 3-9 dpf; after 14 dpf, it was restricted to the outer nuclear layer (ONL). Moreover, we demonstrated for the first time that inaa acted distinctively from the cytoskeletal scaffold of zebrafish cone photoreceptors during development. In conclusion, we demonstrated the morphological features of a novel nIF, inaa, and illustrated its developmental expression pattern in the zebrafish retina. J. Comp. Neurol. 524:3810-3826, 2016. © 2016 Wiley Periodicals, Inc.

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confidence: 99%

Developmental pattern of the neuronal intermediate filament inaa in the zebrafish retina

Liao

Peng

Kan

et al. 2016

J of Comparative Neurology

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“…The exceptions are her8a 33,34 , sall1a 35 , sall3a 35 , sox2 36–39 , sox19b 37,40–42 and sp8b 43,44 . There is also some limited spinal expression data at 24 h for aurkb 45,46 supplementary data , foxb1b 47,48 , nr2f1b 49 supplementary data , sall4 50 , zfhx3b 51 , as well as at stages earlier than those we examine in this paper for foxb1a 47 , foxb1b 52 , myt1a 53 and sall1a 35 . Finally, there are some data at www.ZFIN.org or https://ibcs-bip-web1.ibcs.kit.edu/ffdb/ from large scale zebrafish expression screens, that show spinal cord expression of aurkb , foxb1a, foxb1b, her8a, homeza, ivns1abpb, mybl2b, nr2f1b, onecut1, sall1a, sall3b, sall4, sox2, sox19b, sp8b, tsc22d1, wdhd1 and zfhx3b 6–11 .…”

Section: Discussionmentioning

confidence: 79%

Transcriptional Regulators with Broad Expression in the Zebrafish Spinal Cord

England,

Campbell,

Banerjee

et al. 2024

Preprint

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Background The spinal cord is a crucial part of the vertebrate central nervous system, controlling movements and receiving and processing sensory information from the trunk and limbs. However, there is much we do not know about how this essential organ develops. Here, we describe expression of 22 transcription factor genes in the zebrafish spinal cord. Results We analyzed the spinal cord expression of aurkb, foxb1a, foxb1b, her8a, homeza, ivns1abpb, mybl2b, myt1a, nr2f1b, onecut1, sall1a, sall3a, sall3b, sall4, sox2, sox19b, sp8b, tsc22d1, wdhd1, zfhx3b, znf804a, and znf1032 in wild-type and MIB E3 ubiquitin protein ligase 1 zebrafish embryos. While all of these genes are broadly expressed in the spinal cord, they have distinct expression patterns from one another. Some are predominanatly expressed in progenitor domains, and others in subsets of post-mitotic cells. Given the conservation of spinal cord development, and the transcription factors that regulate it, we expect that these genes will have similar spinal cord expression patterns in other vertebrates, including mammals and humans. Conclusions Our data identify 22 different transcription factors that are strong candidates for playing different roles in spinal cord development. For several of these genes, this is the first published description of their spinal cord expression.

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“…skor1a, skor1b, skor2 , ebf3a , uncx, uncx4.1, lmx1ba and lmx1bb , all encode transcription factors [ 13 , 63 – 68 ]. In contrast, nefma , neff1 and inab encode Neuronal Intermediate Filament (NIF) proteins [ 69 , 70 ]. NIF proteins are not considered classical transcription factors, but they contain an InterPro transcriptional regulator domain and, thus, could function as transcriptional regulators in GRNs.…”

Section: Resultsmentioning

confidence: 99%

Molecular analyses of zebrafish V0v spinal interneurons and identification of transcriptional regulators downstream of Evx1 and Evx2 in these cells

England,

Rusnock,

Mujcic

et al. 2023

Neural Dev

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Background V0v spinal interneurons are highly conserved, glutamatergic, commissural neurons that function in locomotor circuits. We have previously shown that Evx1 and Evx2 are required to specify the neurotransmitter phenotype of these cells. However, we still know very little about the gene regulatory networks that act downstream of these transcription factors in V0v cells. Methods To identify candidate members of V0v gene regulatory networks, we FAC-sorted wild-type and evx1;evx2 double mutant zebrafish V0v spinal interneurons and expression-profiled them using microarrays and single cell RNA-seq. We also used in situ hybridization to compare expression of a subset of candidate genes in evx1;evx2 double mutants and wild-type siblings. Results Our data reveal two molecularly distinct subtypes of zebrafish V0v spinal interneurons at 48 h and suggest that, by this stage of development, evx1;evx2 double mutant cells transfate into either inhibitory spinal interneurons, or motoneurons. Our results also identify 25 transcriptional regulator genes that require Evx1/2 for their expression in V0v interneurons, plus a further 11 transcriptional regulator genes that are repressed in V0v interneurons by Evx1/2. Two of the latter genes are hmx2 and hmx3a. Intriguingly, we show that Hmx2/3a, repress dI2 interneuron expression of skor1a and nefma, two genes that require Evx1/2 for their expression in V0v interneurons. This suggests that Evx1/2 might regulate skor1a and nefma expression in V0v interneurons by repressing Hmx2/3a expression. Conclusions This study identifies two molecularly distinct subsets of zebrafish V0v spinal interneurons, as well as multiple transcriptional regulators that are strong candidates for acting downstream of Evx1/2 to specify the essential functional characteristics of these cells. Our data further suggest that in the absence of both Evx1 and Evx2, V0v spinal interneurons initially change their neurotransmitter phenotypes from excitatory to inhibitory and then, later, start to express markers of distinct types of inhibitory spinal interneurons, or motoneurons. Taken together, our findings significantly increase our knowledge of V0v and spinal development and move us closer towards the essential goal of identifying the complete gene regulatory networks that specify this crucial cell type.

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The Role of inab in Axon Morphology of an Identified Zebrafish Motoneuron

Cited by 7 publications

References 54 publications

Developmental pattern of the neuronal intermediate filament inaa in the zebrafish retina

Developmental pattern of the neuronal intermediate filament inaa in the zebrafish retina

Transcriptional Regulators with Broad Expression in the Zebrafish Spinal Cord

Molecular analyses of zebrafish V0v spinal interneurons and identification of transcriptional regulators downstream of Evx1 and Evx2 in these cells

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