The role of immunohistochemistry in the diagnosis of hyalinizing clear cell carcinoma of the minor salivary gland: a case report

Lai, Guan–Hua; Nemolato, Sonia; Lecca, S; Parodo, Giuseppina; Medda, Celestino; Faa, Gavino

doi:10.4081/1224

Cited by 28 publications

(13 citation statements)

References 23 publications

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“…29 In this study, we did not observe a significant immunoreactivity for Tβ 4 in intratumoral and in peritumoral vessels: Tβ 4 was always expressed in the cytoplasm of tumor cells, in the absence of any reactivity in the connective tissue, as previously reported by our group in developing human organs. 32,33 Outside the tumor cells, Tβ 4 immunostaining was restricted to peritumoral and intratumoral mast cells, a finding already reported in salivary gland tumors 34 and in human skin. 35 Another intriguing finding in our study is the observation of a progressive decrease in the expression of E-cadherin on the cell surface in Tβ 4 -reactive tumor cells undergoing EMT, with disappearance of E-cadherin in isolated spindle tumor cells migrating toward lymphatic and/or blood vessels.…”

Section: Discussionsupporting

confidence: 60%

Thymosin β-4 in colorectal cancer is localized predominantly at the invasion front in tumor cells undergoing epithelial mesenchymal transition

Nemolato

Restivo

Cabras

et al. 2012

Cancer Biology & Therapy

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Objective: Thymosin b-4 (Tb 4 ) is a ubiquitous peptide that plays pivotal roles in the cytoskeletal system and in cell differentiation during embryogenesis. Recently, a role for Tb 4 has been proposed in experimental and human carcinogenesis. This study was aimed at evaluating the correlation between Tb 4 immunoractivity and colorectal cancer, with particular attemption to tumor cells undergoing epithelial-mesenchymal transition. Methods and Results: 86 intestinal biopsies were retrospectively analyzed including 76 colorectal adenocarcinomas with evident features of epithelial-mesenchymal transition, and 10 samples of normal colorectal mucosa. Paraffin sections were immunostained for Tb 4 and for E-cadherin. Total RNA was isolated from frozen specimens obtained, at surgery, from the normal colon mucosa, the deeper regions and the superficial tumor regions in four cases of colon cancer. Tb 4 immunoreactivity was detected in the vast majority (59/76) of colon carcinomas, showing a patchy distribution, with well differentiated areas significantly more reactive than the less differentiated tumor zones. We also noted a zonal pattern in the majority of tumors, characterized by a progressive increase in immunostaining for Tb 4 from the superficial toward the deepest tumor regions. The strongest expression for Tb 4 was frequently detected in invading tumor cells with features of epithelial-mesenchymal transition. The increase in reactivity for Tb 4 matched with a progressive decrease in E-cadherin expression in invading cancer cells. At mRNA level, the differences in Tb 4 expression between the surrounding colon mucosa and the tumors samples were not significant. Conclusions: Our data show that Tb 4 is expressed in the majority of colon cancers, with preferential immunoreactivity in deep tumor regions. The preferential expression of the peptide and the increase in intensity of the immunostaining at the invasion front suggests a possible link between the peptide and the process of epithelial mesenchymal transition, suggesting a role for Tb 4 in colorectal cancer invasion and metastasis.

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Section: Discussionsupporting

confidence: 60%

Thymosin β-4 in colorectal cancer is localized predominantly at the invasion front in tumor cells undergoing epithelial mesenchymal transition

Nemolato

Restivo

Cabras

et al. 2012

Cancer Biology & Therapy

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“…Our review of the literature revealed 130 cases of HCCC among 48 articles . We did exclude a number of articles that have been reported by others to represent HCCC but, in our view, did not provide sufficient histologic description to confirm the diagnosis .…”

Section: Resultsmentioning

confidence: 98%

Hyalinizing clear cell carcinoma of the head and neck: Case series and update

et al. 2015

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“…Mucicarmine stain can be very helpful for highlighting the mucocytes and distinguishing them from the other clear cells. [4]…”

Section: Discussionmentioning

confidence: 99%

Hyalinizing clear cell carcinoma: A rare entity

Baghirath

Kumar

Vinay

2011

J Oral Maxillofac Pathol

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Hyalinizing clear cell carcinoma (HCCC) is an uncommon malignant salivary gland tumor accounting for about 1% of all intra-oral salivary gland tumors. Microscopic diagnosis of clear cell carcinoma may be challenging because of the spectrum of features which frequently overlaps with the other salivary gland tumors that contain clear cells, and thus it may be a diagnosis of exclusion. Here we, report a case of HCCC in a 36 years old female with detailed histological, histochemical and immunohistochemical discussion.

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The role of immunohistochemistry in the diagnosis of hyalinizing clear cell carcinoma of the minor salivary gland: a case report

Cited by 28 publications

References 23 publications

Thymosin β-4 in colorectal cancer is localized predominantly at the invasion front in tumor cells undergoing epithelial mesenchymal transition

Thymosin β-4 in colorectal cancer is localized predominantly at the invasion front in tumor cells undergoing epithelial mesenchymal transition

Hyalinizing clear cell carcinoma of the head and neck: Case series and update

Hyalinizing clear cell carcinoma: A rare entity

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