The role of IGRA in the diagnosis of tuberculosis infection, differentiating from active tuberculosis, and decision making for initiating treatment or preventive therapy of tuberculosis infection

Goletti, Delia; Delogu, Giovanni; Matteelli, Alberto; Migliori, Giovanni Battista

doi:10.1016/j.ijid.2022.02.047

Cited by 70 publications

(61 citation statements)

References 50 publications

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“…Management of TBI is one of the core interventions in the pursuit for TB elimination, as it is focused in preventing the occurrence of active disease and further transmission. 11 …”

mentioning

confidence: 99%

COVID-19 Hampered Diagnosis of TB Infection in France, Italy, Spain and the United Kingdom

García-García

Blanc

Buonsenso

et al. 2022

Archivos de Bronconeumología

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“…Management of TBI is one of the core interventions in the pursuit for TB elimination, as it is focused in preventing the occurrence of active disease and further transmission. 11 …”

mentioning

confidence: 99%

COVID-19 Hampered Diagnosis of TB Infection in France, Italy, Spain and the United Kingdom

García-García

Blanc

Buonsenso

et al. 2022

Archivos de Bronconeumología

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“…Cytokine assays are widely used for TB diagnosis (Pollock et al, 2013;Bernitz et al, 2021). Antigen-specific IFN-g is the most widely recognized biomarker of M. bovis infection in veterinary studies (Smith et al, 2021b) as well as for M. tuberculosis infection in people (Klautau et al, 2018;Goletti et al, 2022). Studies in humans and cattle have also shown that CXCL9, also known as monokine induced by gamma interferon (MIG), has potential value as a biomarker for TB diagnosis and monitoring (Chung et al, 2015;Kumar et al, 2019;Palmer et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Comparison of interferon gamma release assay and CXCL9 gene expression assay for the detection of Mycobacterium bovis infection in African lions (Panthera leo)

Gumbo

Sylvester²,

Parsons³

et al. 2022

Front. Cell. Infect. Microbiol.

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Mycobacterium bovis (M. bovis) infection has been identified in both domestic and wild animals and may threaten the conservation of vulnerable species including African lions (Panthera leo). There is a need to develop accurate antemortem tools for detection of M. bovis infection in African big cat populations for wildlife management and disease surveillance. The aim of this study was to compare the performances of two immunological assays, the QuantiFERON ® -TB Gold Plus (QFT) Mabtech Cat interferon gamma release assay (IGRA) and QFT CXCL9 gene expression assay (GEA), which have both shown diagnostic potential for M. bovis detection in African lions. Lion whole blood (n=47), stimulated using the QFT platform, was used for measuring antigen-specific CXCL9 expression and IFN-g production and to assign M. bovis infection status. A subset (n=12) of mycobacterial culture-confirmed M. bovis infected and uninfected African lions was used to compare the agreement between the immunological diagnostic assays. There was no statistical difference between the proportions of test positive African lions tested by the QFT Mabtech Cat IGRA compared to the QFT CXCL9 GEA. There was also a moderate association between immunological diagnostic assays when numerical results were compared. The majority of lions had the same diagnostic outcome using the paired assays. Although the QFT Mabtech Cat IGRA provides a more standardized, commercially available, and cost-effective test compared to Frontiers in Cellular and Infection Microbiology frontiersin.org 01

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“…Within COVID-19 patients 9 were individuals with tuberculosis infection (TBI). “NO-COVID-19”-controls were healthy donors (n=14) and individuals with tuberculosis infection ( 27 ) (n=15), with no symptoms of COVID-19 and a negative SARS-CoV-2 serology and/or a negative swab for SARS-CoV-2.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the immunomodulatory effects of interleukin-10 on peripheral blood immune cells of COVID-19 patients: Implication for COVID-19 therapy

et al. 2022

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ObjectiveSeveral therapies with immune-modulatory functions have been proposed to reduce the overwhelmed inflammation associated with COVID-19. Here we investigated the impact of IL-10 in COVID-19, through the ex-vivo assessment of the effects of exogenous IL-10 on SARS-CoV-2-specific-response using a whole-blood platform.MethodsTwo cohorts were evaluated: in “study population A”, plasma levels of 27 immune factors were measured by a multiplex (Luminex) assay in 39 hospitalized “COVID-19 patients” and 29 “NO COVID-19 controls” all unvaccinated. In “study population B”, 29 COVID-19 patients and 30 NO COVID-19-Vaccinated Controls (NO COVID-19-VCs) were prospectively enrolled for the IL-10 study. Whole-blood was stimulated overnight with SARS-COV-2 antigens and then treated with IL-10. Plasma was collected and used for ELISA and multiplex assay. In parallel, whole-blood was stimulated and used for flow cytometry analysis.ResultsBaseline levels of several immune factors, including IL-10, were significantly elevated in COVID-19 patients compared with NO COVID-19 subjects in “study population A”. Among them, IL-2, FGF, IFN-γ, and MCP-1 reached their highest levels within the second week of infection and then decreased. To note that, MCP-1 levels remained significantly elevated compared with controls. IL-10, GM-CSF, and IL-6 increased later and showed an increasing trend over time. Moreover, exogenous addition of IL-10 significantly downregulated IFN-γ response and several other immune factors in both COVID-19 patients and NO COVID-19-VCs evaluated by ELISA and a multiplex analysis (Luminex) in “study population B”. Importantly, IL-10 did not affect cell survival, but decreased the frequencies of T-cells producing IFN-γ, TNF-α, and IL-2 (p<0.05) and down-modulated HLA-DR expression on CD8+ and NK cells.ConclusionThis study provides important insights into immune modulating effects of IL-10 in COVID-19 and may provide valuable information regarding the further in vivo investigations.

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The role of IGRA in the diagnosis of tuberculosis infection, differentiating from active tuberculosis, and decision making for initiating treatment or preventive therapy of tuberculosis infection

Cited by 70 publications

References 50 publications

COVID-19 Hampered Diagnosis of TB Infection in France, Italy, Spain and the United Kingdom

COVID-19 Hampered Diagnosis of TB Infection in France, Italy, Spain and the United Kingdom

Comparison of interferon gamma release assay and CXCL9 gene expression assay for the detection of Mycobacterium bovis infection in African lions (Panthera leo)

Evaluation of the immunomodulatory effects of interleukin-10 on peripheral blood immune cells of COVID-19 patients: Implication for COVID-19 therapy

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