The role of<i>Bacillus acidophilus</i>in osteoporosis and its roles in proliferation and differentiation

Chen, Chen; Dong, Baokang; Wang, Yuming; Zhang, Qiang; Wang, Bangmao; Feng, Song; Zhu, Yu

doi:10.1002/jcla.23471

Cited by 17 publications

(12 citation statements)

References 25 publications

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“…We can use these results to develop clinical applications and regulate the inflammation with controlled changes in intestinal butyrate concentration. Although the reported butyrate concentrations in the literature are in both the micromolar range ,− and the millimolar range, ,, we selected the micromolar range of butyrate, since experimental data for biodistribution of butyrate in that range is available. , Our current model is scalable to study the effect of the millimolar range of butyrate.…”

Section: Resultsmentioning

confidence: 99%

“…In contrast, Lucas et al reported 19.55% reduction in osteoclasts and no significant change in osteoblasts. Another experimental observation showed increased proliferation, differentiation, and maturation of osteoblasts and inhibition of osteoclasts’ proliferation due to LGG and butyrate . Some of these variations are certainly explainable by differences in experimental protocols and treatments and characteristics of animal models (e.g., age, sex, strain, and vendor) between studies.…”

Section: Resultsmentioning

confidence: 99%

“…Another experimental observation showed increased proliferation, differentiation, and maturation of osteoblasts and inhibition of osteoclasts' proliferation due to LGG and butyrate. 55 Some of these variations are certainly explainable by differences in experimental protocols and treatments and characteristics of animal models (e.g., age, sex, strain, and vendor) between studies. However, our model was parametrized heavily from the data from Tyagi et al, 18 so the discrepancy was striking.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Mathematical Modeling of the Gut–Bone Axis and Implications of Butyrate Treatment on Osteoimmunology

Islam

Cook

et al. 2021

Ind. Eng. Chem. Res.

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Butyrate, a short-chain fatty acid produced by the gut microbiota, has pivotal roles in the regulation of the immune system. Recent studies have revealed that butyrate increases the differentiation of peripheral regulatory T cells in the gut−bone axis and promotes osteoblasts' bone forming activity. However, the mechanism of the therapeutic benefit of butyrate in bone remodeling remains incompletely understood. Here, we develop a multicompartment mathematical model to quantitatively predict the contribution of butyrate on the expansion of regulatory T cells in the gut, blood, and bone compartments. We investigate the interplay between regulatory T cell-derived TGF-β and CD8+ T cell-derived Wnt-10b with changes in gut butyrate concentration. In addition, we connect our model to a detailed model of bone metabolism to study the impacts of butyrate and Wnt-10b on trabecular bone volume. Our results indicate both direct and indirect immune-mediated impacts of butyrate on bone metabolism.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mathematical Modeling of the Gut–Bone Axis and Implications of Butyrate Treatment on Osteoimmunology

Islam

Cook

et al. 2021

Ind. Eng. Chem. Res.

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“…In particular, for the treatment of postmenopausal osteoporosis, intestinal flora, especially intestinal probiotics, has been proven to be a potential therapeutic strategy [ 49 ]. Among them, Lactobacillus rhamnosus can stimulate bone formation by increasing estrogen [ 50 ]. The study found that prebiotics can increase the number of probiotics such as lactobacilli and butyric acid bacteria to promote the secretion of more short-chain fatty acids, thereby reducing the intestinal PH value and increasing the solubility of calcium in the intestinal lumen, thus increasing the bone mineral content and bone mineral density of young people [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

The Improving Effect and Safety of Probiotic Supplements on Patients with Osteoporosis and Osteopenia: A Systematic Review and Meta-Analysis of 10 Randomized Controlled Trials

Zeng

Yang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Aim. Probiotics are considered to be bone metabolism regulators, and their efficacy as an adjuvant treatment option for osteoporosis is still controversial. The purpose of this study is to compare the available data from randomized controlled trials (RCT) of probiotics in the treatment of osteoporosis and osteopenia. Methods. As of June 2021, databases such as Medline, Embase, Web of Science, and Central Cochrane Library have been used for English-language literature searches and CNKI and China Biomedical Database have been used for Chinese-language literature searches. RevMan 5.3 was used for bias risk assessment, heterogeneity detection, and meta-analysis. This research has been registered in PROSPERO (CRD42020085934). Results. This systematic review and meta-analysis included 10 RCTs involving 1156. Compared with the placebo, the absolute value of lumbar spine’s BMD was not statistically significant (WMD 0.04 (−0.00, 0.09), P = 0.07 , random effect model), while the percentage of lumbar spine’s BMD was higher (SMD 1.16 (0.21, 2.12), P = 0.02 , random effect model). Compared with the control group, the percentage of total hip’s BMD was not statistically significant (SMD 0.52 (−0.69, 1.73), P = 0.40 , random effect model). The safety analysis showed that, compared with control group, the adverse events in the experimental group were not statistically significant (RR 1.02 (0.92, 1.12), P = 0.70 , fixed effect model). Conclusion. Probiotics may be safety supplements to improve the lumbar spine’s BMD of patients with osteoporosis and osteopenia. More large-sample, random-controlled, high-quality RCTs are needed to further verify the effectiveness and safety of probiotics in intervening osteoporosis or osteopenia.

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“…ALP aktivitesinin arttığı OK konsantrasyonunun yükseldiği ve RUNX2, RANK, NFATc1, cathepsin K, DC-STAMP, OSCAR, WNT2 ve CTNNB1 yükseldiği osteoblastlarda gösterilmiştir. Osteoklastlar içinde tersi gerçekleşmiştir (20). İnsan kemik iliği MKH osteoblastik farklanmasında PLLA scaffoldu ile verilen probiyotik nisin tedavisinin osteoblastik farklanmada etkili olduğu gösterilmiştir.…”

unclassified

Probiyotiklerin Kültürde Mezenkimal Kök Hücre Davranışına Etkisi

KARAKAYALI¹,

Önal

TAMER³

et al. 2022

Balıkesir Medical Journal

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Aim: Probiotic (PB) and Mesenchymal Stem Cell (MSC) are new treatment ideas for diseases. Alterations of microbiata made by PB produce different effects. MSC as the scope of cellular therapy takes place in clinical practice in many diseases such as Graft Versus Host (GVD) within the scope of cellular therapy. In this study, the effects of PB application on MSC behavior in culture in terms of proliferation, migration, differentiation and wound healing were investigated. Material and Method: Frozen adipose tissue-derived (AD) MSC cells were cultured and produced at 2.5x104 cells/cm2 in a twelve-well culture dish. They were examined for proliferation by MTT, migration by the closure of the wound after the creation of a scratch, and differentiation by transforming the bone cell osteoblast. NBL Probiotic Gold as PB was used as bacteria which include 2.5x109 Enterococcus faecium, 2.5x109lactobacillus acidophilus, 2.5x109lactobacillus rhamnosus, 2.5x109Bifidobacterium longum, 2.5x109Bifidobacterium bifidum and examined in terms of behavioral parameters for MSC in the same environment. The relationship between PB and MSC was also investigated by Scanning Electron Microscopy (SEM). Result: It was determined that use of PB caused an increase in proliferation, faster wound closure by increase of migration and higher differentiation to osteoblast. The ultrastructural examination of MSC by SEM showed that MSCs interacted with PB are more spread, more adherent, and have more active cell morphology. Conclusion:The results showed that the addition of PB to the culture medium increased the MSC efficiency. It was thought that PB would be more beneficial for the clinic to see of MSC if the same positive effect results in studies with experimental animals.

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The role ofBacillus acidophilusin osteoporosis and its roles in proliferation and differentiation

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 17 publications

References 25 publications

Mathematical Modeling of the Gut–Bone Axis and Implications of Butyrate Treatment on Osteoimmunology

Mathematical Modeling of the Gut–Bone Axis and Implications of Butyrate Treatment on Osteoimmunology

The Improving Effect and Safety of Probiotic Supplements on Patients with Osteoporosis and Osteopenia: A Systematic Review and Meta-Analysis of 10 Randomized Controlled Trials

Probiyotiklerin Kültürde Mezenkimal Kök Hücre Davranışına Etkisi

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