The Role of Hydrogen Sulfide as an Endogenous Vasorelaxant Factor

Wang, Rui; Cheng, Yuanpei; Wu, Lingyun

doi:10.1007/978-1-59259-806-9_19

Cited by 7 publications

(9 citation statements)

References 28 publications

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“…CBS mRNA has not been detected in mammalian (rat and human) vessels and the CBS inhibitor, aminooxyacetic acid (AOA) did not block H 2 S production by rat vessel homogenates (Hosoki et al, 1997;Zhao et al, 2001;Zhao et al, 2003;Wang et al, 2004). However, our findings suggest that in a number of vertebrates, CBS and perhaps other enzymes are important in vascular H 2 S production.…”

Section: Hypoxia and H 2 S Have Similar Effects On E Mmentioning

confidence: 51%

“…CSE mRNA has been identified in human systemic vessels and rat pulmonary arteries; in rat systemic vessels, both CSE mRNA and the 43·kDA CSE protein have been identified (Hosoki et al, 1997;Zhao et al, 2003;Cheng et al, 2004;Wang et al, 2004). We have also found CSE mRNA in systemic arteries, veins and respiratory vessels of trout (R. Wang, R.A.D.…”

Section: Hypoxia and H 2 S Have Similar Effects On E Mmentioning

confidence: 65%

“…Vessels produce H 2 S enzymatically H 2 S production has now been demonstrated in vessels from lamprey (Fig.·4), rainbow trout (Dombkowski, 2006), rat (Hosoki et al, 1997;Zhao et al, 2001;Zhao et al, 2003;Wang et al, 2004) and cow (Fig.·4). This suggests that H 2 S synthesis is a general property of vertebrate vascular smooth muscle.…”

Section: Hypoxia and H 2 S Have Similar Effects On E Mmentioning

confidence: 92%

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Hydrogen sulfide as an oxygen sensor/transducer in vertebrate hypoxic vasoconstriction and hypoxic vasodilation

Olson

Dombkowski

Russell

et al. 2006

Journal of Experimental Biology

253

246

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Section: Hypoxia and H 2 S Have Similar Effects On E Mmentioning

confidence: 51%

Section: Hypoxia and H 2 S Have Similar Effects On E Mmentioning

confidence: 65%

Section: Hypoxia and H 2 S Have Similar Effects On E Mmentioning

confidence: 92%

See 1 more Smart Citation

Hydrogen sulfide as an oxygen sensor/transducer in vertebrate hypoxic vasoconstriction and hypoxic vasodilation

Olson

Dombkowski

Russell

et al. 2006

Journal of Experimental Biology

253

246

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“…H 2 S can be produced directly from Cys desulfuration via the enzymes CSE (also known as CGL; EC 4.4.1.1) and CBS (EC 4.2.1.22) or from Cys transamination to 3-mercaptopyruvate (with ␣-KG, often serving as the amine acceptor) and subsequent desulfuration by the enzyme 3-MST (EC 2.8.1.2) (8,19). CSE is the only desulfurating enzyme that has been identified in mammalian vessels (5,25,35,36), although we have identified CBS mRNA in trout arteries and veins (16). CSE is found in smooth muscle and endothelial cells, and it is especially abundant in pulmonary arteries (5, 25, 34 -36).…”

Section: H 2 S and O 2 Sensingmentioning

confidence: 99%

Hypoxic pulmonary vasodilation: a paradigm shift with a hydrogen sulfide mechanism

Olson

Whitfield

Bearden

et al. 2010

American Journal of Physiology-Regulatory, Integrative and Comp

130

145

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Hypoxic pulmonary vasoconstriction (HVC), an intrinsic and assumed ubiquitous response of mammalian pulmonary blood vessels, matches regional ventilation to perfusion via an unknown O(2)-sensing mechanism. Global pulmonary hypoxia experienced by individuals suffering from chronic obstructive pulmonary disease or numerous hypoventilation syndromes, including sleep apnea, often produces maladaptive pulmonary hypertension, but pulmonary hypertension is not observed in diving mammals, where profound hypoxia is routine. Here we examined the response of cow and sea lion pulmonary arteries (PA) to hypoxia and observed the expected HVC in the former and a unique hypoxic vasodilation in resistance vessels in the latter. We then used this disparate response to examine the O(2)-sensing mechanism. In both animals, exogenous H(2)S mimicked the vasoactive effects of hypoxia in isolated PA. H(2)S-synthesizing enzymes, cystathionine beta-synthase, cystathionine gamma-lyase, and 3-mercaptopyruvate sulfur transferase, were identified in lung tissue from both animals by one-dimensional Western blot analysis and immunohistochemistry. The relationship between H(2)S production/consumption and O(2) was examined in real time by use of amperometric H(2)S and O(2) sensors. H(2)S was produced by sea lion and cow lung homogenate in the absence of O(2), but it was rapidly consumed when O(2) was present. Furthermore, consumption of exogenous H(2)S by cow lung homogenate, PA smooth muscle cells, and heart mitochondria was O(2) dependent and exhibited maximal sensitivity at physiologically relevant Po(2) levels. These studies show that HVC is not an intrinsic property of PA and provide further evidence for O(2)-dependent H(2)S metabolism in O(2) sensing.

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“…H 2 S-mediated vasodilation has been demonstrated in mammalian systemic vessels and at least part of this response is due to H 2 S opening of ATP sensitive potassium (K ATP ) channels on the vascular smooth muscle cell and to release of nitric oxide (NO) from the endothelium (Zhao et al, 2001;Zhao and Wang, 2002;Wang et al, 2004). H 2 S-mediated vasoconstriction has been demonstrated in mammalian pulmonary vessels and in a variety of both pulmonary and systemic vessels from non-mammalian vertebrates (Dombkowski et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Oxygen dependency of hydrogen sulfide-mediated vasoconstriction in cyclostome aortas

Olson

Forgan

Dombkowski

et al. 2008

Journal of Experimental Biology

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SUMMARYHydrogen sulfide (H 2 S) has been proposed to mediate hypoxic vasoconstriction (HVC), however, other studies suggest the vasoconstrictory effect indirectly results from an oxidation product of H 2 S. Here we examined the relationship between H 2 S and O 2 in isolated hagfish and lamprey vessels that exhibit profound hypoxic vasoconstriction. In myographic studies, H 2 S (Na 2 S) dose-dependently constricted dorsal aortas (DA) and efferent branchial arteries (EBA) but did not affect ventral aortas or afferent branchial arteries; effects similar to those produced by hypoxia. Sensitivity of H 2 S-mediated contraction in hagfish and lamprey DA was enhanced by hypoxia. HVC in hagfish DA was enhanced by the H 2 S precursor cysteine and inhibited by amino-oxyacetate, an inhibitor of the H 2 S-synthesizing enzyme, cystathionine β-synthase. HVC was unaffected by propargyl glycine, an inhibitor of cystathionine λ-lyase. Oxygen consumption (M O 2) of hagfish DA was constant between 15 and 115·mmHg P O 2 (1·mmHg=0.133·kPa), decreased when P O 2 <15·mmHg, and increased after P O 2 exceeded 115·mmHg. 10·μmol·l -1 H 2 S increased and у100·μmol·l -1 H 2 S decreased M O 2. Consistent with the effects on HVC, cysteine increased and amino-oxyacetate decreased M O 2. These results show that H 2 S is a monophasic vasoconstrictor of specific cyclostome vessels and because hagfish lack vascular NO, and vascular sensitivity to H 2 S was enhanced at low P O 2, it is unlikely that H 2 S contractions are mediated by either H 2 S-NO interaction or an oxidation product of H 2 S. These experiments also provide additional support for the hypothesis that the metabolism of H 2 S is involved in oxygen sensing/signal transduction in vertebrate vascular smooth muscle.

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The Role of Hydrogen Sulfide as an Endogenous Vasorelaxant Factor

Cited by 7 publications

References 28 publications

Hydrogen sulfide as an oxygen sensor/transducer in vertebrate hypoxic vasoconstriction and hypoxic vasodilation

Hydrogen sulfide as an oxygen sensor/transducer in vertebrate hypoxic vasoconstriction and hypoxic vasodilation

Hypoxic pulmonary vasodilation: a paradigm shift with a hydrogen sulfide mechanism

Oxygen dependency of hydrogen sulfide-mediated vasoconstriction in cyclostome aortas

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